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. 1975 Sep;16(3):736–740. doi: 10.1128/jvi.16.3.736-740.1975

Molecular weight of DNA from actinophage MSP2.

W D Lancaster, L A Jones
PMCID: PMC354723  PMID: 1159900

Abstract

Actinophage MSP2 is infectious for Streptomyces venezuelae S13. Based upon electron microscopy of coliphage T4 mixed with MSP2, MSP2 had a head about 48 +/- 2 nm wide and 87 +/- 5 nm long. DNA from polyoma virus and from coliphages T4 and T7 served as reference markers in estimating the molecular weight of MSP2 DNA from sedimentation in sucrose gradients. Denatured MSP2 DNA was estimated to be about 17 x 10(6) and double-stranded MSP2 DNA was about (36 +/- 1.6) x 10(6) in molecular weight.

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Selected References

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  1. Anderson C. W., Eigner J. Breakdown and exclusion of superinfecting T-even bacteriophage in Escherichia coli. J Virol. 1971 Dec;8(6):869–886. doi: 10.1128/jvi.8.6.869-886.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BURGI E., HERSHEY A. D. Sedimentation rate as a measure of molecular weight of DNA. Biophys J. 1963 Jul;3:309–321. doi: 10.1016/s0006-3495(63)86823-x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bradley D. E. Ultrastructure of bacteriophage and bacteriocins. Bacteriol Rev. 1967 Dec;31(4):230–314. doi: 10.1128/br.31.4.230-314.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bradley S. G., Jones L. A. Bacteriophages, their biology and industrial significance. Prog Ind Microbiol. 1968;7:43–75. [PubMed] [Google Scholar]
  5. Dubin S. B., Benedek G. B., Bancroft F. C., Freifelder D. Molecular weights of coliphages and colip- hage DNA. II. Measurement of diffusion coefficients using optical mixing spectroscopy, and measurement of sedimentation coefficients. J Mol Biol. 1970 Dec 28;54(3):547–556. doi: 10.1016/0022-2836(70)90125-7. [DOI] [PubMed] [Google Scholar]
  6. Germaine G. R., Anderson D. L. Binding of homologous polymerized deoxyribonucleic acid by Streptomyces griseus. J Bacteriol. 1966 Sep;92(3):662–667. doi: 10.1128/jb.92.3.662-667.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ito J., Spizizen J. Abortive infection of sporulating Bacillus subtilis 168 by phi 2 bacteriophage. J Virol. 1971 Apr;7(4):515–523. doi: 10.1128/jvi.7.4.515-523.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jones L. A., Bradley S. G. The life-cycle of an actionophage for Streptomyces venezuelae. J Gen Microbiol. 1965 Aug;40(2):191–198. doi: 10.1099/00221287-40-2-191. [DOI] [PubMed] [Google Scholar]
  9. Jones L. A., Hahn M. L., Taylor A. R. Incomplete and aberrant particles of actinophage MSP2 from lysates of Streptomyces venezuelae. J Virol. 1974 Sep;14(3):493–502. doi: 10.1128/jvi.14.3.493-502.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kim J. S., Davidson N. Electron microscope heteroduplex study of sequence relations of T2, T4, and T6 bacteriophage DNAs. Virology. 1974 Jan;57(1):93–111. doi: 10.1016/0042-6822(74)90111-1. [DOI] [PubMed] [Google Scholar]
  11. Okanishi M., Utahara R., Okami Y. Infection of the protoplasts of Streptomyces kanamyceticus with deoxyribonucleic acid preparation from actinophage PK-66. J Bacteriol. 1966 Dec;92(6):1850–1852. doi: 10.1128/jb.92.6.1850-1852.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  13. Vinograd J., Lebowitz J., Radloff R., Watson R., Laipis P. The twisted circular form of polyoma viral DNA. Proc Natl Acad Sci U S A. 1965 May;53(5):1104–1111. doi: 10.1073/pnas.53.5.1104. [DOI] [PMC free article] [PubMed] [Google Scholar]

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