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. 1975 Oct;16(4):880–886. doi: 10.1128/jvi.16.4.880-886.1975

Characterization of endogenous RNA-directed DNA polymerase activity of reticuloendotheliosis viruses.

C Y Kang
PMCID: PMC354749  PMID: 51936

Abstract

Reticuloendotheliosis viruses (REV) contain an endogenous RNA-directed DNA polymerase activity. The endogenous DNA polymerase activity can be elicited in purified preparations of REV by treatment with nonionic detergents. The enzyme activity has a strong preference for manganous ions. Therefore, appreciable endogenous DNA polymerase activity can be demonstrated only if the reaction mixture contains appropriate concentrations of manganous ions. Enzyme activity can be inhibited by pretreatment with RNase or deletion of one or more deoxyribonucleoside triphosphates from the reaction mixture. In contrast, actinomycin D has little effect in initial DNA synthesis. The results from both velocity and equilibrium centrifugation indicate that the nascent chains of product DNA are associated with 60S viral RNA. The DNA product of the endogenous DNA polymerase reaction is hybridizable to REV RNA, but not to avian leukosis virus RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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