Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1975 Oct;16(4):913–926. doi: 10.1128/jvi.16.4.913-926.1975

Morphogenesis of Sindbis virus in cultured Aedes albopictus cells.

J B Gliedman, J F Smith, D T Brown
PMCID: PMC354753  PMID: 170422

Abstract

Cultured mosquito cells were found to produce Sindbis virus nearly as efficiently as BHK-21 cells at 28 C. In virtually all of the cells observed in the electron microscope, virus morphogenesis was found to occur within complex vesicular structures which developed after viral infection. Viral nucleocapsids were first seen in these vesicles and appeared to be enveloped within these structures. The process of envelopment within these inclusions differed in some respects from the process previously described for the envelopment of nucleocapsids at the plasma membrane of vertebrae cells. Free nucleocapsids were only rarely seen in the cytoplasm of infected mosquito cells, and budding of virus from the cell surface was detected so infrequently that this process of virus production could not account for the amount of virus produced by the infected cells. The vast majority of extracellular virus was produced by the fusion of the virus-containing vesicles with the plasma membrane releasing mature virions and membrane nucleocapsid complexes in various stages of development.

Full text

PDF
913

Images in this article

915Image
on p.915
916Image
on p.916
917Image
on p.917
918Image
on p.918
919Image
on p.919
920Image
on p.920
921Image
on p.921
922Image
on p.922
922Image
on p.922
923Image
on p.923
924Image
on p.924
925Image
on p.925

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acheson N. H., Tamm I. Replication of Semliki Forest virus: an electron microscopic study. Virology. 1967 May;32(1):128–143. doi: 10.1016/0042-6822(67)90261-9. [DOI] [PubMed] [Google Scholar]
  2. Brown D. T., Gliedman J. B. Morphological variants of Sindbis virus obtained from infected mosquito tissue culture cells. J Virol. 1973 Dec;12(6):1534–1539. doi: 10.1128/jvi.12.6.1534-1539.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brown D. T., Waite M. R., Pfefferkorn E. R. Morphology and morphogenesis of Sindbis virus as seen with freeze-etching techniques. J Virol. 1972 Sep;10(3):524–536. doi: 10.1128/jvi.10.3.524-536.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bykovsky A. F., Yershov F. I., Zhdanov V. M. Morphogenesis of Venezuelan equine encephalomyelitis virus. J Virol. 1969 Oct;4(4):496–504. doi: 10.1128/jvi.4.4.496-504.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  6. Filshie B. K., Rehacek J. Studies of the morphology of Murray Valley encephalitis and Japanese encephalitis viruses growing in cultured mosquito cells. Virology. 1968 Mar;34(3):435–443. doi: 10.1016/0042-6822(68)90063-9. [DOI] [PubMed] [Google Scholar]
  7. Hopwood D. A comparison of the crosslinking abilities of glutaraldehyde, formaldehyde and alpha-hydroxyadipaldehyde with bovine serum albumin and casein. Histochemie. 1969;17(2):151–161. doi: 10.1007/BF00277781. [DOI] [PubMed] [Google Scholar]
  8. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Marchesi V. T., Jackson R. L., Segrest J. P., Kahane I. Molecular features of the major glycoprotein of the human erythrocyte membrane. Fed Proc. 1973 Aug;32(8):1833–1837. [PubMed] [Google Scholar]
  10. Matsumura T., Stollar V., Schlesinger R. W. Studies on the nature of dengue viruses. V. Structure and development of dengue virus in Vero cells. Virology. 1971 Nov;46(2):344–355. doi: 10.1016/0042-6822(71)90036-5. [DOI] [PubMed] [Google Scholar]
  11. PFEFFERKORN E. R., CLIFFORD R. L. THE ORIGIN OF THE PROTEIN OF SINDBIS VIRUS. Virology. 1964 Jun;23:217–223. doi: 10.1016/0042-6822(64)90285-5. [DOI] [PubMed] [Google Scholar]
  12. Raghow R. S., Davey M. W., Dalgarno L. The growth of Semliki Forest virus in cultured mosquito cells: ultrastructural observations. Arch Gesamte Virusforsch. 1973;43(1):165–168. doi: 10.1007/BF01249360. [DOI] [PubMed] [Google Scholar]
  13. Raghow R. S., Grace T. D., Filshie B. K., Bartley W., Dalgarno L. Ross River virus replication in cultured mosquito and mammalian cells: virus growth and correlated ultrastructural changes. J Gen Virol. 1973 Oct;21:109–122. doi: 10.1099/0022-1317-21-1-109. [DOI] [PubMed] [Google Scholar]
  14. Sefton B. M., Gaffney B. J. Effect of the viral proteins on the fluidity of the membrane lipids in Sindbis virus. J Mol Biol. 1974 Dec 5;90(2):343–358. doi: 10.1016/0022-2836(74)90378-7. [DOI] [PubMed] [Google Scholar]
  15. Stevens T. M. Arbovirus replication in mosquito cell lines (Singh) grown in monolayer or suspension culture. Proc Soc Exp Biol Med. 1970 May;134(1):356–361. doi: 10.3181/00379727-134-34793. [DOI] [PubMed] [Google Scholar]
  16. Tillack T. W., Scott R. E., Marchesi V. T. The structure of erythrocyte membranes studied by freeze-etching. II. Localization of receptors for phytohemagglutinin and influenza virus to the intramembranous particles. J Exp Med. 1972 Jun 1;135(6):1209–1227. doi: 10.1084/jem.135.6.1209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Waite M. R., Brown D. T., Pfefferkorn E. R. Inhibition of Sindbis virus release by media of low ionic strength: an electron microscope study. J Virol. 1972 Sep;10(3):537–544. doi: 10.1128/jvi.10.3.537-544.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES