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Journal of Virology logoLink to Journal of Virology
. 1975 Oct;16(4):1010–1016. doi: 10.1128/jvi.16.4.1010-1016.1975

Genetic analysis of bacteriophage phi29 of Bacillus subtilis: mapping of the cistrons coding for structural proteins.

B E Reilly, M E Tosi, D L Anderson
PMCID: PMC354763  PMID: 809595

Abstract

Four phage phi29 suppressor-sensitive mutants of cistron O have been examined for production of 14C-labeled viral-specific proteins in restrictive infections of Bacillus subtilis and fail to produce the protein of the viral neck lower collar. Cistrons O and F have been placed on the genetic map, containing 12 cistrons, by three-factor crosses. The phenotypes of five cistron J mutants have been analyzed by sodium dodecyl sulfate gel electrophoresis and autoradiography, and in three instances fragments of the normal polypeptide were detected. Three factor crosses with these mutants and a virus with a clear plaque phenotype were used to initiate the mapping of cistron J and the determination of the orientation of transcription in this map region.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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