Abstract
Four phage phi29 suppressor-sensitive mutants of cistron O have been examined for production of 14C-labeled viral-specific proteins in restrictive infections of Bacillus subtilis and fail to produce the protein of the viral neck lower collar. Cistrons O and F have been placed on the genetic map, containing 12 cistrons, by three-factor crosses. The phenotypes of five cistron J mutants have been analyzed by sodium dodecyl sulfate gel electrophoresis and autoradiography, and in three instances fragments of the normal polypeptide were detected. Three factor crosses with these mutants and a virus with a clear plaque phenotype were used to initiate the mapping of cistron J and the determination of the orientation of transcription in this map region.
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Selected References
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- Anderson D. L., Hickman D. D., Reilly B. E. Structure of Bacillus subtilis bacteriophage phi 29 and the length of phi 29 deoxyribonucleic acid. J Bacteriol. 1966 May;91(5):2081–2089. doi: 10.1128/jb.91.5.2081-2089.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Anderson D. L., Mosharrafa E. T. Physical and biological properties of phage phi 29 deoxyribonucleic acid. J Virol. 1968 Oct;2(10):1185–1190. doi: 10.1128/jvi.2.10.1185-1190.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Anderson D. L., Reilly B. E. Analysis of bacteriophage phi 29 gene function: protein synthesis in suppressor-sensitive mutant infection of Bacillus subtilis. J Virol. 1974 Jan;13(1):211–221. doi: 10.1128/jvi.13.1.211-221.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Camacho A., Moreno F., Carrascosa J. L., Viñuela E., Salas M. A suppressor of nonsense mutations in Bacillus subtilis. Eur J Biochem. 1974 Aug 15;47(1):199–205. doi: 10.1111/j.1432-1033.1974.tb03683.x. [DOI] [PubMed] [Google Scholar]
- Carrascosa J. L., Camacho A., Viñuela E., Salas M. A precursor of the neck appendage protein of B. subtilis phage phi 29. FEBS Lett. 1974 Aug 30;44(3):317–321. [PubMed] [Google Scholar]
- Carrascosa J. L., Viñuela E., Salas M. Proteins induced in Bacillus subtilis infected with bacteriophage phi 29. Virology. 1973 Nov;56(1):291–299. [PubMed] [Google Scholar]
- Georgopoulos C. P. Suppressor system in Bacillus subtilis 168. J Bacteriol. 1969 Mar;97(3):1397–1402. doi: 10.1128/jb.97.3.1397-1402.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hawley L. A., Reilly B. E., Hagen E. W., Anderson D. L. Viral protein synthesis in bacteriophage phi 29-infected Bacillus subtilis. J Virol. 1973 Nov;12(5):1149–1159. doi: 10.1128/jvi.12.5.1149-1159.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loskutoff D. J., Pène J. J. Gene expression during the development of Bacillus subtilis bacteriophage phi29. II. Resolution of viral-specific ribonucleic acid molecules. J Virol. 1973 Jan;11(1):87–97. doi: 10.1128/jvi.11.1.87-97.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McGuire J. C., Pène J. J., Barrow-Carraway J. Gene expression during the development of bacteriophage phi 29. 3. Analysis of viral-specific protein synthesis with suppressible mutants. J Virol. 1974 Mar;13(3):690–698. doi: 10.1128/jvi.13.3.690-698.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moreno F. Suppressor-sensitive mutants and genetic map of Bacillus subtilis bacteriophage phi 29. Virology. 1974 Nov;62(1):1–16. doi: 10.1016/0042-6822(74)90298-0. [DOI] [PubMed] [Google Scholar]
- Méndez E., Ramírez G., Salas M., Viñuela E. Structural proteins of bacteriophage phi 29. Virology. 1971 Sep;45(3):567–576. doi: 10.1016/0042-6822(71)90172-3. [DOI] [PubMed] [Google Scholar]
- Ortin J., Viñuela E., Salas M., Vasquez C. DNA-protein complex in circular DNA from phage phi-29. Nat New Biol. 1971 Dec 29;234(52):275–277. doi: 10.1038/newbio234275a0. [DOI] [PubMed] [Google Scholar]
- Polsinelli M., Beretta M. Genetic Recombination in Crosses Between Streptomyces aureofaciens and Streptomyces rimosus. J Bacteriol. 1966 Jan;91(1):63–68. doi: 10.1128/jb.91.1.63-68.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Péne J. J., Murr P. C., Barrow-Carraway J. Synthesis of bacteriophage phi 29 proteins in Bacillus subtilis. J Virol. 1973 Jul;12(1):61–67. doi: 10.1128/jvi.12.1.61-67.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REILLY B. E., SPIZIZEN J. BACTERIOPHAGE DEOXYRIBONUCLEATE INFECTION OF COMPETENT BACILLUS SUBTILIS. J Bacteriol. 1965 Mar;89:782–790. doi: 10.1128/jb.89.3.782-790.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reilly B. E., Zeece V. M., Anderson D. L. Genetic study of suppressor-sensitive mutants of the Bacillus subtilis bacteriophage phi 29. J Virol. 1973 May;11(5):756–760. doi: 10.1128/jvi.11.5.756-760.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schachtele C. F., De Sain C. V., Anderson D. L. Transcription during the development of bacteriophage phi29: definition of "early" and "late" phi29 ribonucleic acid. J Virol. 1973 Jan;11(1):9–16. doi: 10.1128/jvi.11.1.9-16.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tosi M., Anderson D. L. Antigenic properties of bacteriophage phi 29 structural proteins. J Virol. 1973 Dec;12(6):1548–1559. doi: 10.1128/jvi.12.6.1548-1559.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]