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. 1975 Oct;16(4):1085–1089. doi: 10.1128/jvi.16.4.1085-1089.1975

Intragenic Complementation by Gene 42 Amber Mutations of Bacteriophage T4

George E Holmes a,1
PMCID: PMC354772  PMID: 1172535

Abstract

Phage T4 amber mutants defective in gene 42 (dCMP hydroxymethylase) were shown by in vivo and in vitro experiments to participate in both positive and negative intragenic complementation. This argues that incomplete polypeptide chains can participate in subunit interaction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Campbell A. The steric effect in lysogenization by bacteriophage lambda. I. Lysogenization of a partially diploid strain of Escherichia coli K-12. Virology. 1965 Nov;27(3):329–339. doi: 10.1016/0042-6822(65)90112-1. [DOI] [PubMed] [Google Scholar]
  3. Chiu C. S., Greenberg G. R. Evidence for a possible direct role of dCMP hydroxymethylase in T4 phage DNA synthesis. Cold Spring Harb Symp Quant Biol. 1968;33:351–359. doi: 10.1101/sqb.1968.033.01.041. [DOI] [PubMed] [Google Scholar]
  4. Chiu C. S., Greenberg G. R. Intragenic complementation between temperature-sensitive mutants of gene 42 (dCMP hydroxymethylase) of bacteriophage T4. J Virol. 1973 Aug;12(2):415–416. doi: 10.1128/jvi.12.2.415-416.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FRASER D., JERREL E. A. The amino acid composition of T3 bacteriophage. J Biol Chem. 1953 Nov;205(1):291–295. [PubMed] [Google Scholar]
  6. MATHEWS C. K., SUTHERLAND K. E. COMPARATIVE BIOCHEMISTRY OF BACTERIAL AND PHAGE-INDUCED DIHYDROFOLATE REDUCTASES. J Biol Chem. 1965 May;240:2142–2147. [PubMed] [Google Scholar]
  7. Mathews C. K., Kessin R. H. Control of bacteriophage-induced enzyme synthesis in cells infected with a temperature-sensitive mutant. J Virol. 1967 Feb;1(1):92–96. doi: 10.1128/jvi.1.1.92-96.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. O'Farrell P. Z., Gold L. M., Huang W. M. The identification of prereplicative bacteriophage T4 proteins. J Biol Chem. 1973 Aug 10;248(15):5499–5501. [PubMed] [Google Scholar]
  9. PIZER L. I., COHEN S. S. Virus-induced acquisition of metabolic function. V. Purification and properties of the deoxycytidylate hydroxymethylase and studies on its origin. J Biol Chem. 1962 Apr;237:1251–1259. [PubMed] [Google Scholar]
  10. Tomich P. K., Greenberg G. R. On the defect of a dCMP hydroxymethylase mutant of bacteriophage T4 showing enzyme activity in extracts. Biochem Biophys Res Commun. 1973 Feb 20;50(4):1032–1038. doi: 10.1016/0006-291x(73)91510-6. [DOI] [PubMed] [Google Scholar]
  11. WIBERG J. S., BUCHANAN J. M. STUDIES ON LABILE DEOXYCYTIDYLATE HYDROXYMETHYLASES FROM ESCHERICHIA COLI B INFECTED WITH TEMPERATURE-SENSITIVE MUTANTS OF BACTERIOPHAGE T4. Proc Natl Acad Sci U S A. 1964 Mar;51:421–428. doi: 10.1073/pnas.51.3.421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Yegian C. D., Mueller M., Selzer G., Russo V., Stahl F. W. Properties of the DNA-delay mutants of bacteriophage T4. Virology. 1971 Dec;46(3):900–919. doi: 10.1016/0042-6822(71)90090-0. [DOI] [PubMed] [Google Scholar]

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