Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1976 Jun;18(3):848–855. doi: 10.1128/jvi.18.3.848-855.1976

Isolation and preliminary characterization of temperature-sensitive mutants of Newcastle disease virus.

J E Tsipis, M A Bratt
PMCID: PMC354783  PMID: 1271532

Abstract

Temperature-sensitive (ts) mutants of Newcastle disease virus have been isolated and characterized genetically (complementation), biochemically (RNA synthesis) and biologically (fusion from within and hemadsorption). Fifteen of these mutants have been divided into five complementation groups. Groups A (five mutants) and E (one mutant) are ts for RNA synthesis (RNA-) as well as for the other functions. Group B contains four RNA+ mutants of which one is ts for fusion, one for hemadsorption and two for neither function. Group C contains one RNA+ mutant which is a poor cell fuser. Group D contains two RNA+ mutants which are ts for fusion. In addition, two noncomplementing mutants (group BC) fail to complement both group B and group C mutants while exhibiting complementation with mutants in groups A, D, and E.

Full text

PDF
848

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bergholz C. M., Kiley M. P., Payne F. E. Isolation and characterization of temperature-sensitive mutants of measles virus. J Virol. 1975 Jul;16(1):192–202. doi: 10.1128/jvi.16.1.192-202.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bratt M. A., Gallaher W. R. Preliminary analysis of the requirements for fusion from within and fusion from without by Newcastle disease virus. Proc Natl Acad Sci U S A. 1969 Oct;64(2):536–543. doi: 10.1073/pnas.64.2.536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bratt M. A. RNA synthesis in NDV-infected chick embryo cells treated with different concentrations of actinomycin D. Virology. 1969 Sep;39(1):141–145. doi: 10.1016/0042-6822(69)90357-2. [DOI] [PubMed] [Google Scholar]
  4. Burge B. W., Pfefferkorn E. R. Isolation and characterization of conditional-lethal mutants of Sindbis virus. Virology. 1966 Oct;30(2):204–213. doi: 10.1016/0042-6822(66)90096-1. [DOI] [PubMed] [Google Scholar]
  5. Clavell L. A., Bratt M. A. Hemolytic interaction of Newcastle disease virus and chicken erythrocytes. II. Determining factors. Appl Microbiol. 1972 Mar;23(3):461–470. doi: 10.1128/am.23.3.461-470.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Collins B. S., Bratt M. A. Separation of the messenger RNAs of Newcastle disease virus by gel electrophoresis. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2544–2548. doi: 10.1073/pnas.70.9.2544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Duesberg P. H., Robinson W. S. Isolation of the nucleic acid of Newcastle disease virus (NDV). Proc Natl Acad Sci U S A. 1965 Sep;54(3):794–800. doi: 10.1073/pnas.54.3.794. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Flamand A. Etude génétique du virus de la stomatite vésiculaire: classement de mutants thermosensibles spontanés en groupes de complémentation. J Gen Virol. 1970 Sep;8(3):187–195. doi: 10.1099/0022-1317-8-3-187. [DOI] [PubMed] [Google Scholar]
  9. Flamand A., Pringle C. R. The homologies of spontaneous and induced temperature-sensitive mutants of vesicular stomatitis virus isolated in chick embryo and BHK 21 cells. J Gen Virol. 1971 May;11(2):81–85. doi: 10.1099/0022-1317-11-2-81. [DOI] [PubMed] [Google Scholar]
  10. Gallaher W. R., Bratt M. A. Conditional dependence of fusion from within and other cell membrane alterations by Newcastle disease virus. J Virol. 1974 Oct;14(4):813–820. doi: 10.1128/jvi.14.4.813-820.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hightower L. E., Bratt M. A. Protein synthesis in Newcastle disease virus-infected chicken embryo cells. J Virol. 1974 Apr;13(4):788–800. doi: 10.1128/jvi.13.4.788-800.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Holloway A. F., Wong P. K., Cormack D. V. Isolation and characterization of temperature-sensitive mutants of vesicular stomatitis virus. Virology. 1970 Dec;42(4):917–926. doi: 10.1016/0042-6822(70)90340-5. [DOI] [PubMed] [Google Scholar]
  13. Kingsbury D. W. Newcastle disease virus RNA. I. Isolation and preliminary characterization of RNA from virus particles. J Mol Biol. 1966 Jun;18(1):195–203. doi: 10.1016/s0022-2836(66)80085-2. [DOI] [PubMed] [Google Scholar]
  14. Kirvaitis J., Simon E. H. A radiobiological study of the development of Newcastle disease virus. Virology. 1965 Aug;26(4):545–553. doi: 10.1016/0042-6822(65)90316-8. [DOI] [PubMed] [Google Scholar]
  15. Kolakofsky D., Boy de la Tour E., Delius H. Molecular weight determination of Sendai and Newcastle disease virus RNA. J Virol. 1974 Feb;13(2):261–268. doi: 10.1128/jvi.13.2.261-268.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. Lafay F. Etude des mutants thermosensibles du Virus de la Stomatite Vésiculaire (VSV). Classification de quelques mutants d'après des critères de fonctionnement. C R Acad Sci Hebd Seances Acad Sci D. 1969 May 12;268(19):2385–2388. [PubMed] [Google Scholar]
  18. Martinet C., Printz Ane C. Analyse de la synthèse de l'ARN viral du virus de la stomatite vésiculaire (VSV). Utilisation de mutants thermosensibles. Ann Inst Pasteur (Paris) 1970 Oct;119(4):411–419. [PubMed] [Google Scholar]
  19. Portner A., Marx P. A., Kingsbury D. W. Isolation and characterization of Sendai virus temperature-sensitive mutants. J Virol. 1974 Feb;13(2):298–304. doi: 10.1128/jvi.13.2.298-304.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Portner A., Scroggs R. A., Marx P. S., Kingsbury D. W. A temperature-sensitive mutant of Sendai virus with an altered hemagglutinin-neuraminidase polypeptide: consequences for virus assembly and cytopathology. Virology. 1975 Sep;67(1):179–187. doi: 10.1016/0042-6822(75)90415-8. [DOI] [PubMed] [Google Scholar]
  21. Preble O. T., Youngner J. S. Temperature-sensitive defect of mutants isolated from L cells persistently infected with Newcastle disease virus. J Virol. 1973 Sep;12(3):472–480. doi: 10.1128/jvi.12.3.472-480.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Preble O. T., Youngner J. S. Temperature-sensitive mutants isolated from L cells persistently infected with Newcastle disease virus. J Virol. 1972 Feb;9(2):200–206. doi: 10.1128/jvi.9.2.200-206.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pringle C. R., Duncan I. B. Preliminary physiological characterization of temperature-sensitive mutants of vesicular stomatitis virus. J Virol. 1971 Jul;8(1):56–61. doi: 10.1128/jvi.8.1.56-61.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Pringle C. R. Genetic characteristics of conditional lethal mutants of vesicular stomatitis virus induced by 5-fluorouracil, 5-azacytidine, and ethyl methane sulfonate. J Virol. 1970 May;5(5):559–567. doi: 10.1128/jvi.5.5.559-567.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Repik P., Flamand A., Clark H. F., Obijeski J. F., Roy P., Bishop D. H. Detection of homologous RNA sequences among six rhabdovirus genomes. J Virol. 1974 Jan;13(1):250–252. doi: 10.1128/jvi.13.1.250-252.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Weiss S. R., Bratt M. A. Comparative electrophoresis of the 18-22S RNAs of Newcastle disease virus. J Virol. 1976 Apr;18(1):316–323. doi: 10.1128/jvi.18.1.316-323.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Weiss S. R., Bratt M. A. Polyadenylate sequences on Newcastle disease virus mRNA synthesized in vivo and in vitro. J Virol. 1974 Jun;13(6):1220–1230. doi: 10.1128/jvi.13.6.1220-1230.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES