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. 1976 Jun;18(3):894–903. doi: 10.1128/jvi.18.3.894-903.1976

Identification of gene products required for in vitro formation of the internal peptides of bacteriophage T4.

J G Giri, J E McCullough, S P Champe
PMCID: PMC354788  PMID: 1271534

Abstract

In vitro formation of both bacteriophage T4 internal peptides (II and VII) from preexisting precursor protein was shown to require the product of T4 gene 21. The proteolytic factor was detectable in extracts of cells infected with certain phage mutants blocked in early steps of head assembly but could not be demonstrated in extracts of T4 wild-type infected cells. This finding suggests that the proteolytic factor is inactivated during normal phage assembly. The product of T4 gene 22 appears to be the precursor of peptide VII but not of peptide II.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachrach U., Benchetrit L. Studies on phage internal proteins. II. Cleavage of a precursor of internal proteins during the morphogenesis of bacteriophage T4. Virology. 1974 May;59(1):51–58. doi: 10.1016/0042-6822(74)90205-0. [DOI] [PubMed] [Google Scholar]
  2. Black L. W. Bacteriophage T4 internal protein mutants: isolation and properties. Virology. 1974 Jul;60(1):166–179. doi: 10.1016/0042-6822(74)90374-2. [DOI] [PubMed] [Google Scholar]
  3. Bolin R. W., Cummings D. J. Structural aberrations in T-even bacteriophage. VII. In vitro analysis of the canavanine-mediated inhibition of proteolytic cleavage. J Virol. 1975 Nov;16(5):1273–1281. doi: 10.1128/jvi.16.5.1273-1281.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dickson R. C., Barnes S. L., Eiserling F. A. Structural proteins of bacteriophage T4. J Mol Biol. 1970 Nov 14;53(3):461–474. doi: 10.1016/0022-2836(70)90077-x. [DOI] [PubMed] [Google Scholar]
  5. EDGAR R. S., DENHARDT G. H., EPSTEIN R. H. A COMPARATIVE GENETIC STUDY OF CONDITIONAL LETHAL MUTATIONS OF BACTERIOPHAGE T4D. Genetics. 1964 Apr;49:635–648. doi: 10.1093/genetics/49.4.635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Eddleman H. L., Champe S. P. Components in T4-infected cells associated with phage assembly. Virology. 1966 Nov;30(3):471–481. doi: 10.1016/0042-6822(66)90123-1. [DOI] [PubMed] [Google Scholar]
  7. Goldstein J., Champe S. P. T4-induced activity required for specific cleavage of a bacteriophage protein in vitro. J Virol. 1974 Feb;13(2):419–427. doi: 10.1128/jvi.13.2.419-427.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hosoda J., Cone R. Analysis of T4 phage proteins. I. Conversion of precursor proteins into lower molecular weight peptides during normal capsid formation. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1275–1281. doi: 10.1073/pnas.66.4.1275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hosoda J., Levinthal C. Protein synthesis by Escherichia coli infected with bacteriophage T4D. Virology. 1968 Apr;34(4):709–727. doi: 10.1016/0042-6822(68)90092-5. [DOI] [PubMed] [Google Scholar]
  10. Kellenberger E., Der Kamp C. K.-V. On a modification of the gene product P23 according to its use as subunit of either normal capsids of phage T4 or of polyheads. FEBS Lett. 1970 Jun 1;8(3):140–144. doi: 10.1016/0014-5793(70)80247-2. [DOI] [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Laemmli U. K., Paulson J. R., Hitchins V. Maturation of the head of bacteriophage T4. V. A possible DNA packaging mechanism: in vitro cleavage of the head proteins and the structure of the core of the polyhead. J Supramol Struct. 1974;2(2-4):276–301. doi: 10.1002/jss.400020219. [DOI] [PubMed] [Google Scholar]
  13. Onorato L., Showe M. K. Gene 21 protein-dependent proteolysis in vitro of purified gene 22 product of bacteriophage T4. J Mol Biol. 1975 Mar 5;92(3):395–412. doi: 10.1016/0022-2836(75)90288-0. [DOI] [PubMed] [Google Scholar]
  14. Showe M. K., Black L. W. Assembly core of bacteriophage T4: an intermediate in head formation. Nat New Biol. 1973 Mar 21;242(116):70–75. doi: 10.1038/newbio242070a0. [DOI] [PubMed] [Google Scholar]
  15. Sternberg N., Champe S. P. Genetic determinant of an internal peptide of bacteriophage T4. J Mol Biol. 1969 Dec 28;46(3):377–392. doi: 10.1016/0022-2836(69)90183-1. [DOI] [PubMed] [Google Scholar]

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