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. 1976 Jun;18(3):942–949. doi: 10.1128/jvi.18.3.942-949.1976

Novel replicative properties of a capsid mutant of bacteriophage phi chi 174.

C C Vito, C E Dowell
PMCID: PMC354794  PMID: 775131

Abstract

A capsid mutant of bacteriophage phi chi 174 demonstrates altered requirements for the conversion of viral single-stranded DNA to double-stranded replicative form DNA. In the presence of puromycin at 42 C, wild-type phi chi 174 is unable to complete this replicative event, whereas phi chi ahb is able to do so. Furthermore, in contrast to wild-type phi chi 174, formation of phi chi ahb parental replicative form DNA is sensitive to rifampin under certain experimental conditions. These data suggest that the mutant capsid proteins of phi chi ahb influence the biosynthesis of phi chi ahb complementary strand DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bone D. R., Dowell C. E. A mutant of bacteriophage øX174 which infects E. coli K12 strains. II. Replication of øXtB in tsDNA strains. Virology. 1973 Apr;52(2):330–336. doi: 10.1016/0042-6822(73)90327-9. [DOI] [PubMed] [Google Scholar]
  2. Chamberlin M. Isolation and characterization of prototrophic mutants of Escherichia coli unable to support the intracellular growth of T7. J Virol. 1974 Sep;14(3):509–516. doi: 10.1128/jvi.14.3.509-516.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Doniger J., Tessman I. An S13 capsid mutant that makes no replicative-form DNA. Virology. 1969 Nov;39(3):389–394. doi: 10.1016/0042-6822(69)90086-5. [DOI] [PubMed] [Google Scholar]
  4. Dumas L. B., Miller C. A., Bayne M. L. Rifampin inhibition of bacteriophage phiX174 parental replicative-form DNA synthesis in an Escherichia coli dnaC mutant. J Virol. 1975 Sep;16(3):575–580. doi: 10.1128/jvi.16.3.575-580.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dumas L. B., Miller C. A. Inhibition of bacteriophage phi chi 174 DNA replication in dnaB mutants of Escherichia coli C. J Virol. 1974 Dec;14(6):1369–1379. doi: 10.1128/jvi.14.6.1369-1379.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Geider K., Kornberg A. Conversion of the M13 viral single strand to the double-stranded replicative forms by purified proteins. J Biol Chem. 1974 Jul 10;249(13):3999–4005. [PubMed] [Google Scholar]
  7. Haworth S. R., Gilgun C. F., Dowell C. E. Growth studies of three phi chi 174 mutants in tsDNA mutants Escherichia coli. J Virol. 1975 Apr;15(4):720–725. doi: 10.1128/jvi.15.4.720-725.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jazwinski S. M., Marco R., Kornberg A. A coat protein of the bacteriophage M13 virion participates in membrane-oriented synthesis of DNA. Proc Natl Acad Sci U S A. 1973 Jan;70(1):205–209. doi: 10.1073/pnas.70.1.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jazwinski S. M., Marco R., Kornberg A. The gene H spike protein of bacteriophages phiX174 and S13. II. Relation to synthesis of the parenteral replicative form. Virology. 1975 Jul;66(1):294–305. doi: 10.1016/0042-6822(75)90199-3. [DOI] [PubMed] [Google Scholar]
  10. Marco R., Jazwinski S. M., Kornberg A. Binding, eclipse, and penetration of the filamentous bacteriophage M13 in intact and disrupted cells. Virology. 1974 Nov;62(1):209–223. doi: 10.1016/0042-6822(74)90316-x. [DOI] [PubMed] [Google Scholar]
  11. McFadden G., Denhardt D. T. Mechanism of replication of phi x174 single-stranded DNA. IX. Requirement for the Escherichia coli dnaG protein. J Virol. 1974 Nov;14(5):1070–1075. doi: 10.1128/jvi.14.5.1070-1075.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mitra S., Stallions D. R. Role of dna genes of Escherichia coli in M13 phage replication. Virology. 1973 Apr;52(2):417–424. doi: 10.1016/0042-6822(73)90336-x. [DOI] [PubMed] [Google Scholar]
  13. Modrich P., Richardson C. C. Bacteriophage T7 Deoxyribonucleic acid replication in vitro. A protein of Escherichia coli required for bacteriophage T7 DNA polymerase activity. J Biol Chem. 1975 Jul 25;250(14):5508–5514. [PubMed] [Google Scholar]
  14. Modrich P., Richardson C. C. Bacteriophage T7 deoxyribonucleic acid replication invitro. Bacteriophage T7 DNA polymerase: an an emzyme composed of phage- and host-specific subunits. J Biol Chem. 1975 Jul 25;250(14):5515–5522. [PubMed] [Google Scholar]
  15. NATHANS D. PUROMYCIN INHIBITION OF PROTEIN SYNTHESIS: INCORPORATION OF PUROMYCIN INTO PEPTIDE CHAINS. Proc Natl Acad Sci U S A. 1964 Apr;51:585–592. doi: 10.1073/pnas.51.4.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pero J., Nelson J., Fox T. D. Highly asymmetric transcription by RNA polymerase containing phage-SP01-induced polypeptides and a new host protein. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1589–1593. doi: 10.1073/pnas.72.4.1589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Primrose S. B., Landers W. M., Dowell C. E. Replication of bacteriophage phiX174 in a temperature-sensitive deoxyribonucleic acid host cell. J Virol. 1971 Oct;8(4):594–596. doi: 10.1128/jvi.8.4.594-596.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ray D. S., Dueber J., Suggs S. Replication of bacteriophage M13 IX. Requirement of the Escherichia coli dnaG function for M13 duplex DNA replication. J Virol. 1975 Aug;16(2):348–355. doi: 10.1128/jvi.16.2.348-355.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schekman R., Weiner A., Kornberg A. Multienzyme systems of DNA replication. Science. 1974 Dec 13;186(4168):987–993. doi: 10.1126/science.186.4168.987. [DOI] [PubMed] [Google Scholar]
  20. Schekman R., Weiner J. H., Weiner A., Kornberg A. Ten proteins required for conversion of phiX174 single-stranded DNA to duplex form in vitro. Resolution and reconstitution. J Biol Chem. 1975 Aug 10;250(15):5859–5865. [PubMed] [Google Scholar]
  21. Schekman R., Wickner W., Westergaard O., Brutlag D., Geider K., Bertsch L. L., Kornberg A. Initiation of DNA synthesis: synthesis of phiX174 replicative form requires RNA synthesis resistant to rifampicin. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2691–2695. doi: 10.1073/pnas.69.9.2691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Silverstein S., Billen D. Transcription: role in the initiation and replication of DNA synthesis in Escherichia coli and phiX174. Biochim Biophys Acta. 1971 Oct;247(3):383–390. doi: 10.1016/0005-2787(71)90023-2. [DOI] [PubMed] [Google Scholar]
  23. Sinsheimer R. L., Knippers R., Komano T. Stages in the replication of bacteriophage phi X174 DNA in vivo. Cold Spring Harb Symp Quant Biol. 1968;33:443–447. doi: 10.1101/sqb.1968.033.01.051. [DOI] [PubMed] [Google Scholar]
  24. Snyder L. R., Montgomery D. L. Inhibition of T4 growth by an RNA polymerase mutation of Escherichia coli: physiological and genetic analysis of the effects during phage development. Virology. 1974 Nov;62(1):184–196. doi: 10.1016/0042-6822(74)90314-6. [DOI] [PubMed] [Google Scholar]
  25. Steinberg R. A., Denhardt D. T. Inhibition of synthesis of phiX174 DNA in a mutant host thermosensitive for DNA synthesis. J Mol Biol. 1968 Nov 14;37(3):525–528. doi: 10.1016/0022-2836(68)90120-4. [DOI] [PubMed] [Google Scholar]
  26. Sunshine M. G., Sauer B. A bacterial mutation blocking P2 phage late gene expression. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2770–2774. doi: 10.1073/pnas.72.7.2770. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sunshine M., Usher D., Calendar R. Interaction of P2 bacteriophage with the dnaB gene of Escherichia coli. J Virol. 1975 Aug;16(2):284–289. doi: 10.1128/jvi.16.2.284-289.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Taketo A. Sensitivity of Escherichia coli to viral nucleic acid. VI. Capacity of dna mutants and DNA polymerase-less mutants for multiplication of phiA and phiX 174. Mol Gen Genet. 1973 Mar 27;122(1):15–22. doi: 10.1007/BF00337970. [DOI] [PubMed] [Google Scholar]
  29. Vito C. C., Primrose S. B., Dowell C. E. Growth of a capsid mutant of bacteriophage phi X174 in a temperature-sensitive strain of Escherichia coli. J Virol. 1975 Feb;15(2):281–287. doi: 10.1128/jvi.15.2.281-287.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wickner S., Hurwitz J. Association of phiX174 DNA-dependent ATPase activity with an Escherichia coli protein, replication factor Y, required for in vitro synthesis of phiX174 DNA. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3342–3346. doi: 10.1073/pnas.72.9.3342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Wickner S., Hurwitz J. Conversion of phiX174 viral DNA to double-stranded form by purified Escherichia coli proteins. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4120–4124. doi: 10.1073/pnas.71.10.4120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Wickner W., Brutlag D., Schekman R., Kornberg A. RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form. Proc Natl Acad Sci U S A. 1972 Apr;69(4):965–969. doi: 10.1073/pnas.69.4.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Wickner W., Kornberg A. A novel form of RNA polymerase from Escherichia coli. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4425–4428. doi: 10.1073/pnas.71.11.4425. [DOI] [PMC free article] [PubMed] [Google Scholar]

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