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. 1976 Jun;18(3):1155–1159. doi: 10.1128/jvi.18.3.1155-1159.1976

Translation of T7 RNA in vitro without cleavage by RNase III.

Y Yamada, D Nakada
PMCID: PMC354818  PMID: 775130

Abstract

T7 early mRNA's are generated from a high-molecular-weight precursor RNA by site-specific RNase III cleavage. When T7 DNA is transcribed in vitro by Escherichia coli RNA polymerase, the transcript is a large, single-piece RNA equivalent to the in vivo precursor RNA. The T7 RNA synthesized in vitro can be translated as a polycistronic messenger without cleavage by RNase III. All T7 early proteins are synthesized in an RNase III-free, protein-synthesizing system directed by the uncleaved T7 RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chamberlin M., McGrath J., Waskell L. New RNA polymerase from Escherichia coli infected with bacteriophage T7. Nature. 1970 Oct 17;228(5268):227–231. doi: 10.1038/228227a0. [DOI] [PubMed] [Google Scholar]
  2. Crouch R. J. Ribonuclease 3 does not degrade deoxyribonucleic acid-ribonucleic acid hybrids. J Biol Chem. 1974 Feb 25;249(4):1314–1316. [PubMed] [Google Scholar]
  3. Dunn J. J., Studier F. W. Effect of RNAase III, cleavage on translation of bacteriophage T7 messenger RNAs. J Mol Biol. 1975 Dec 15;99(3):487–499. doi: 10.1016/s0022-2836(75)80140-9. [DOI] [PubMed] [Google Scholar]
  4. Dunn J. J., Studier F. W. T7 early RNAs and Escherichia coli ribosomal RNAs are cut from large precursor RNAs in vivo by ribonuclease 3. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3296–3300. doi: 10.1073/pnas.70.12.3296. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dunn J. J., Studier F. W. T7 early RNAs are generated by site-specific cleavages. Proc Natl Acad Sci U S A. 1973 May;70(5):1559–1563. doi: 10.1073/pnas.70.5.1559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hercules K., Schweiger M., Sauerbier W. Cleavage by RNase 3 converts T3 and T7 early precursor RNA into translatable message. Proc Natl Acad Sci U S A. 1974 Mar;71(3):840–844. doi: 10.1073/pnas.71.3.840. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Herrlich P., Schweiger M. DNA- and RNA-directed synthesis in vitro of phage enzymes. Methods Enzymol. 1974;30:654–668. doi: 10.1016/0076-6879(74)30063-8. [DOI] [PubMed] [Google Scholar]
  8. Hesselbach B. A., Yamada Y., Nakada D. Isolation of an inhibitor protein of E. coli RNA polymerase from T7 phage infected cell. Nature. 1974 Nov 1;252(5478):71–74. doi: 10.1038/252071b0. [DOI] [PubMed] [Google Scholar]
  9. Jay G., Kaempfer R. Initiation of protein synthesis. Binding of messenger RNA. J Biol Chem. 1975 Aug 10;250(15):5742–5748. [PubMed] [Google Scholar]
  10. Kramer R. A., Rosenberg M., Steitz J. A. Nucleotide sequences of the 5' and 3' termini of bacteriophage T7 early messenger RNAs synthesized in vivo: evidence for sequence specificity in RNA processing. J Mol Biol. 1974 Nov 15;89(4):767–776. doi: 10.1016/0022-2836(74)90051-5. [DOI] [PubMed] [Google Scholar]
  11. Minkley E. G., Jr Transcription of the early region of bacteriophage T7: characterization of the in vivo transcripts. J Mol Biol. 1974 Mar;83(3):289–304. doi: 10.1016/0022-2836(74)90281-2. [DOI] [PubMed] [Google Scholar]
  12. Minkley E. G., Jr Transcription of the early region of bacteriophage T7: specificity and selectivity in the in vitro initiation of RNA synthesis. J Mol Biol. 1974 Mar;83(3):305–331. doi: 10.1016/0022-2836(74)90282-4. [DOI] [PubMed] [Google Scholar]
  13. Minkley E. G., Pribnow D. Transcription of the early region of bacteriophage T7: selective initiation with dinucleotides. J Mol Biol. 1973 Jun 25;77(2):255–277. doi: 10.1016/0022-2836(73)90335-5. [DOI] [PubMed] [Google Scholar]
  14. Nikolaev N., Silengo L., Schlessinger D. A role for ribonuclease 3 in processing of ribosomal ribonucleic acid and messenger ribonucleic acid precursors in Escherichia coli. J Biol Chem. 1973 Nov 25;248(22):7967–7969. [PubMed] [Google Scholar]
  15. Robertson H. D., Webster R. E., Zinder N. D. Purification and properties of ribonuclease III from Escherichia coli. J Biol Chem. 1968 Jan 10;243(1):82–91. [PubMed] [Google Scholar]
  16. Rosenberg M., Kramer R. A., Steitz J. A. T7 early messenger RNAs are the direct products of ribonuclease III cleavage. J Mol Biol. 1974 Nov 15;89(4):777–782. doi: 10.1016/0022-2836(74)90052-7. [DOI] [PubMed] [Google Scholar]
  17. Studier F. W. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973 Sep 15;79(2):237–248. doi: 10.1016/0022-2836(73)90003-x. [DOI] [PubMed] [Google Scholar]
  18. Studier F. W. Bacteriophage T7. Science. 1972 Apr 28;176(4033):367–376. doi: 10.1126/science.176.4033.367. [DOI] [PubMed] [Google Scholar]
  19. Yamada Y., Nakada D. Early to late switch in bacteriophage T7 development: no translational discrimination between T7 early messenger RNA and late messenger RNA. J Mol Biol. 1976 Jan 5;100(1):35–45. doi: 10.1016/s0022-2836(76)80032-0. [DOI] [PubMed] [Google Scholar]
  20. Yamada Y., Whitaker P. A., Nakada D. Chemical stability of bacteriophage T7 early mRNA. J Virol. 1975 Dec;16(6):1683–1687. doi: 10.1128/jvi.16.6.1683-1687.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yamada Y., Whitaker P. A., Nakada D. Early to late switch in bacteriophage T7 development: functional decay of T7 early messenger RNA. J Mol Biol. 1974 Oct 25;89(2):293–303. doi: 10.1016/0022-2836(74)90520-8. [DOI] [PubMed] [Google Scholar]

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