Abstract
The microviscosity of the hydrophobic region of the membrane of infectious hematopoietic necrosis virus was determined using fluorescence depolarization analysis of the probe 1,6-diphenyl-1,3,5-hexatriene and was found to be much lower at 37 C than that of another rhabdovirus, vesicular stomatitis virus. However, the microviscosity of this fish virus at 18 C, the temperature at which it was grown, corresponded to the microviscosity of vesicular stomatitis virus at 37 C. Data obtained with the fish virus host cell (chinook salmon embryo cells) grown at 18 C suggest that its membranes have a lower microviscosity than either L-929 or BHK-21 cells (the vesicular stomatitis virus host cells) grown at 37 C.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
- McAllister P. E., Wagner R. R. Structural proteins of two salmonid rhabdoviruses. J Virol. 1975 Apr;15(4):733–738. doi: 10.1128/jvi.15.4.733-738.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McCain B. B., Fryer J. L., Pilcher K. S. Antigenic relationships in a group of three viruses of salmonid fish by cross neutralization. Proc Soc Exp Biol Med. 1971 Jul;137(3):1042–1046. doi: 10.3181/00379727-137-35724. [DOI] [PubMed] [Google Scholar]
- Moore N. F., Barenholz Y., Wagner R. R. Microviscosity of togavirus membranes studied by fluorescence depolarization: influence of envelope proteins and the host cell. J Virol. 1976 Jul;19(1):126–135. doi: 10.1128/jvi.19.1.126-135.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schloemer R. H., Wagner R. R. Mosquito cells infected with vesicular stomatitis virus yield unsialylated virions of low infectivity. J Virol. 1975 Apr;15(4):1029–1032. doi: 10.1128/jvi.15.4.1029-1032.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shinitzky M., Barenholz Y. Dynamics of the hydrocarbon layer in liposomes of lecithin and sphingomyelin containing dicetylphosphate. J Biol Chem. 1974 Apr 25;249(8):2652–2657. [PubMed] [Google Scholar]
- Wingfield W. H., Fryer J. L., Pilcher K. S. Properties of the sockeye salmon virus (Oregon strain). Proc Soc Exp Biol Med. 1969 Apr;130(4):1055–1059. doi: 10.3181/00379727-130-33719. [DOI] [PubMed] [Google Scholar]