Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1976 Aug;19(2):409–415. doi: 10.1128/jvi.19.2.409-415.1976

Viral DNA-synthesizing intermediate complex isolated during assembly of bacteriophage phi X174.

H Fujisawa, M Hayashi
PMCID: PMC354878  PMID: 957476

Abstract

A DNA protein complex that is a precursor of mature phi X174 phage was isolated. The complex sedimented with an S value of 50 in a sucrose gradient and contained phage DNA consisting of a replicative form molecule with an extended tail of single-stranded viral DNA. The viral-strand DNA ranged from one to two genomes in length. Proteins coded on the phi X174 genome as well as the host genome were associated with the viral DNA in the 50S precursor complex. Our results indicated that both viral DNA synthesis and cleavage of the growing viral-strand DNA occurred in the 50S complex.

Full text

PDF
409

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ando T. A nuclease specific for heat-denatured DNA in isolated from a product of Aspergillus oryzae. Biochim Biophys Acta. 1966 Jan 18;114(1):158–168. doi: 10.1016/0005-2787(66)90263-2. [DOI] [PubMed] [Google Scholar]
  2. Denhardt D. T., Dressler D. H., Hathaway A. THE ABORTIVE REPLICATION OF PhiX174 DNA IN A RECOMBINATION-DEFICIENT MUTANT OF Escherichia coli. Proc Natl Acad Sci U S A. 1967 Mar;57(3):813–820. doi: 10.1073/pnas.57.3.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Denhardt D. T., Iwaya M., McFadden G., Schochetman G. The mechanism of replication of phi X174 single-stranded DNA. VI. Requirements for ribonucleoside triphosphates and DNA polymerase 3. Can J Biochem. 1973 Dec;51(12):1588–1597. doi: 10.1139/o73-214. [DOI] [PubMed] [Google Scholar]
  4. Denhardt D. T., Sinsheimer R. L. The process of infection with bacteriophage phi-X174. IV. Replication of the viral DNA in a synchronized infection. J Mol Biol. 1965 Jul;12(3):647–662. doi: 10.1016/s0022-2836(65)80319-9. [DOI] [PubMed] [Google Scholar]
  5. Dowell C. E., Sinsheimer R. L. The process of infection with bacteriophage phi-X174. IX. Studies on the physiology of three phi-X174 temperature-sensitive mutants. J Mol Biol. 1966 Apr;16(2):374–386. doi: 10.1016/s0022-2836(66)80180-8. [DOI] [PubMed] [Google Scholar]
  6. Dressler D. H., Denhardt D. T. Mechanism of replication of phi-X-174 single stranded DNA. Nature. 1968 Jul 27;219(5152):346–351. doi: 10.1038/219346a0. [DOI] [PubMed] [Google Scholar]
  7. Dumas L. B., Miller C. A. Inhibition of bacteriophage phi chi 174 DNA replication in dnaB mutants of Escherichia coli C. J Virol. 1974 Dec;14(6):1369–1379. doi: 10.1128/jvi.14.6.1369-1379.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Francke B., Ray D. S. Cis-limited action of the gene-A product of bacteriophage phiX174 and the essential bacterial site (E. coli-electron microscopy-cis-acting protein-specifically-nicked RF). Proc Natl Acad Sci U S A. 1972 Feb;69(2):475–479. doi: 10.1073/pnas.69.2.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fujisawa H., Hayashi M. Gene A product of phi X174 is required for site-specific endonucleolytic cleavage during single-stranded DNA synthesis in vivo. J Virol. 1976 Aug;19(2):416–424. doi: 10.1128/jvi.19.2.416-424.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gelfand D. H., Hayashi M. Electrophoretic characterization of phiX174-specific proteins. J Mol Biol. 1969 Sep 28;44(3):501–516. doi: 10.1016/0022-2836(69)90376-3. [DOI] [PubMed] [Google Scholar]
  11. Gilbert W., Dressler D. DNA replication: the rolling circle model. Cold Spring Harb Symp Quant Biol. 1968;33:473–484. doi: 10.1101/sqb.1968.033.01.055. [DOI] [PubMed] [Google Scholar]
  12. Greenlee L. L. Replication of bacteriophage phiX-174 in a mutant of Escherichia coli defective in the dnaE gene. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1757–1760. doi: 10.1073/pnas.70.6.1757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hayashi Y., Hayashi M. Template activities of the phi X-174 replicative allomorphic deoxyribonucleic acids. Biochemistry. 1971 Nov;10(23):4212–4218. doi: 10.1021/bi00799a009. [DOI] [PubMed] [Google Scholar]
  14. Henry T. J., Knippers R. Isolation and function of the gene A initiator of bacteriophage phi-chi 174, a highly specific DNA endonuclease. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1549–1553. doi: 10.1073/pnas.71.4.1549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hutchison C. A., 3rd, Sinsheimer R. L. The process of infection with bacteriophage phi-X174. X. Mutations in a phi-X Lysis gene. J Mol Biol. 1966 Jul;18(3):429–447. doi: 10.1016/s0022-2836(66)80035-9. [DOI] [PubMed] [Google Scholar]
  16. Iwaya M., Denhardt D. T. The mechanism of replication of phi X174 single-stranded DNA. II. The role of viral proteins. J Mol Biol. 1971 Apr 28;57(2):159–175. doi: 10.1016/0022-2836(71)90339-1. [DOI] [PubMed] [Google Scholar]
  17. Knippers R., Razin A., Davis R., Sinsheimer R. L. The process of infection with Bacteriophage phi-X174. XXIX. In vivo studies on the synthesis of the single-stranded DNA of progeny phi-X174 bacteriophage. J Mol Biol. 1969 Oct 28;45(2):237–263. doi: 10.1016/0022-2836(69)90103-x. [DOI] [PubMed] [Google Scholar]
  18. Kranias E. G., Dumas L. B. Replication of bacteriophage phiX174 DNA in a temperature-sensitive dnaC mutant of escherichia coli C. J Virol. 1974 Jan;13(1):146–154. doi: 10.1128/jvi.13.1.146-154.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lindqvist B. H., Sinsheimer R. L. Process of infection with bacteriophage phi-X174. XIV. Studies on macromolecular synthesis during infection with a lysis-defective mutant. J Mol Biol. 1967 Aug 28;28(1):87–94. doi: 10.1016/s0022-2836(67)80079-2. [DOI] [PubMed] [Google Scholar]
  20. Lindqvist B. H., Sinsheimer R. L. The process of infection with bacteriophage phi-X174. XV. Bacteriophage DNA synthesis in abortive infections with a set of conditional lethal mutants. J Mol Biol. 1967 Nov 28;30(1):69–80. doi: 10.1016/0022-2836(67)90244-6. [DOI] [PubMed] [Google Scholar]
  21. Linney E., Hayashi M. Intragenic regulation of the synthesis of phi chi 174 gene A proteins. Nature. 1974 May 24;249(455):345–348. doi: 10.1038/249345a0. [DOI] [PubMed] [Google Scholar]
  22. Linney E., Hayashi M. Two proteins of gene A of psiX174. Nat New Biol. 1973 Sep 5;245(140):6–8. doi: 10.1038/newbio245006a0. [DOI] [PubMed] [Google Scholar]
  23. McFadden G., Denhardt D. T. Mechanism of replication of phi x174 single-stranded DNA. IX. Requirement for the Escherichia coli dnaG protein. J Virol. 1974 Nov;14(5):1070–1075. doi: 10.1128/jvi.14.5.1070-1075.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. SINSHEIMER R. L., STARMAN B., NAGLER C., GUTHRIE S. The process of infection with bacteriophage phi-XI74. I. Evidence for a "replicative form". J Mol Biol. 1962 Mar;4:142–160. doi: 10.1016/s0022-2836(62)80047-3. [DOI] [PubMed] [Google Scholar]
  25. Schekman R. W., Iwaya M., Bromstrup K., Denhardt D. T. The mechanism of replication of phi X174 single-stranded DNA. 3. An enzymic study of the structure of the replicative form II DNA. J Mol Biol. 1971 Apr 28;57(2):177–199. doi: 10.1016/0022-2836(71)90340-8. [DOI] [PubMed] [Google Scholar]
  26. Schekman R. W., Ray D. S. Polynucleotide ligase and phiX174 single strand synthesis. Nat New Biol. 1971 Jun 9;231(23):170–173. doi: 10.1038/newbio231170a0. [DOI] [PubMed] [Google Scholar]
  27. Siden E. J., Hayashi M. Role of the gene beta-product in bacteriophage phi-X174 development. J Mol Biol. 1974 Oct 15;89(1):1–16. doi: 10.1016/0022-2836(74)90159-4. [DOI] [PubMed] [Google Scholar]
  28. Siegel J. E., Hayashi M. Phi-X-174 bacteriophage structural mutants which affect deoxyribonucleic acid synthesis. J Virol. 1969 Oct;4(4):400–407. doi: 10.1128/jvi.4.4.400-407.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Sinsheimer R. L. Bacteriophage phi-X174 and related viruses. Prog Nucleic Acid Res Mol Biol. 1968;8:115–169. [PubMed] [Google Scholar]
  30. Sinsheimer R. L., Knippers R., Komano T. Stages in the replication of bacteriophage phi X174 DNA in vivo. Cold Spring Harb Symp Quant Biol. 1968;33:443–447. doi: 10.1101/sqb.1968.033.01.051. [DOI] [PubMed] [Google Scholar]
  31. Tessman E. S. Mutants of bacteriophage S13 blocked in infectious DNA synthesis. J Mol Biol. 1966 May;17(1):218–236. doi: 10.1016/s0022-2836(66)80104-3. [DOI] [PubMed] [Google Scholar]
  32. Tonegawa S., Hayashi M. Intermediates in the assembly of phi X174. J Mol Biol. 1970 Mar 14;48(2):219–242. doi: 10.1016/0022-2836(70)90158-0. [DOI] [PubMed] [Google Scholar]
  33. Weisbeek P. J., Sinsheimer R. L. A DNA-protein complex involved in bacteriophage phi chi 174 particle formation. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3054–3058. doi: 10.1073/pnas.71.8.3054. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES