Abstract
The existence of two components of human leukocyte interferon has been recently reported. In the present study, the nature of this molecular heterogeneity was explored by affinity chromatography on immobilized micro- and macroligands, ion-exchange chromatography, and molecular sieving. Chromatography on a series of alkyl-agarose adsorbents shows, for the first time, the intrinsic hydrophobicity of human leukocyte interferon. Additionally, the separation of two interferon components is achieved by use of the alkyl-agarose as well as by the omega-aminoalkyl-agarose adsorbents. Clear-cut separation of the two components was also achieved by chromatography on BSA-CH-Sepharose and on DEAE-Bio Gel A. An important feature of these separations is that they do not require the use of denaturing conditions. The molecular weights of the leukocyte interferon components, as determined on Sephadex G-75, are quite similar or identical, approximately 26,000. Thus, the molecular heterogeneity of human leukocyte interferon can be attributed, at least in part, to differences in the hydrophobicity and ionic properties of its two components.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anfinsen C. B., Bose S., Corley L., Gurari-Rotman D. Partial purification of human interferon by affinity chromatography. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3139–3142. doi: 10.1073/pnas.71.8.3139. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carter W. A., Pitha P. M., Marshall L. W., Tazawa I., Tazawa S., Ts'o P. O. Structural requirements of the rI n -rC n complex for induction of human interferon. J Mol Biol. 1972 Oct 14;70(3):567–587. doi: 10.1016/0022-2836(72)90560-8. [DOI] [PubMed] [Google Scholar]
- Huang J. W., Davey M. W., Hejna C. J., Von Muenchhausen W., Sulkowski E., Carter W. A. Selective binding of human interferon to albumin immoblized on agarose. J Biol Chem. 1974 Jul 25;249(14):4665–4667. [PubMed] [Google Scholar]
- Huang J. W., Hejna C. I., Sulkowski E., Carter W. A., Silver G. H., Munayyer H., Came P. The human interferon-albumin interaction: the influence of albumin conformation. Virology. 1975 May;65(1):268–271. doi: 10.1016/0042-6822(75)90028-8. [DOI] [PubMed] [Google Scholar]
- Jankowski W. J., Davey M. W., O'Malley J. A., Sulkowski E., Carter W. A. Molecular structure of human fibroblast and leukocyte interferons: probe by lectin and hydrophobic chromatography. J Virol. 1975 Nov;16(5):1124–1130. doi: 10.1128/jvi.16.5.1124-1130.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stewart K. K., Doherty R. F. Resolution of DL-tryptophan by affinity chromatography on bovine-serum albumin-agarose columns. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2850–2852. doi: 10.1073/pnas.70.10.2850. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stewart W. E., 2nd, Desmyter J. Molecular heterogeneity of human leukocyte interferon: two populations differing in molecular weights, requirements for renaturation, and cross-species antiviral activity. Virology. 1975 Sep;67(1):68–73. doi: 10.1016/0042-6822(75)90403-1. [DOI] [PubMed] [Google Scholar]