Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
pKa Values for Wild-type Fmsl and the D94N and N195A Enzymesa
| enzyme | eq | kinetic parameter | pK1 | pK2 |
|---|---|---|---|---|
| wild-type Fms1b | 1 | kcat/Kamine | 8.3 ± 0.1 | 9.6 ± 0.1 |
| 2 | k cat | 7.7 ± 0.1 | — | |
| N195A | 1 | kcat/Kamine | 8.1 ± 0.1 | 9.4 ± 0.2 |
| 2 | k cat | 9.0 ± 0.1 | — | |
| D94N | 1 | kcat/Kamine | 8.7 ± 0.1 | 8.7 ± 0.1 |
| 2 | k cat | 8.4 ± 0.1 | — |
Conditions: N1-acetylspermine as a substrate, 25 °C.
From ref. (13).
