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. 1976 Aug;19(2):610–619. doi: 10.1128/jvi.19.2.610-619.1976

RNA polymerase activity in purified virions of avian reticuloendotheliosis viruses.

S Mizutani, H M Temin
PMCID: PMC354896  PMID: 183017

Abstract

An RNA polymerase activity that synthesizes a U-rich RNA hydrogen bonded to a large viral RNA molecule was found in the cores of virions of avian reticuloendotheliosis viruses (REV). The RNA polymerase activity was separable from the DNA polymerase activity of REV virions. The 5'-terminus of the newly synthesized RNA was A. In addition, a tRNA nucleotidyl transferase activity, which added -CpCpA ends to tRNA, appears to be present in the REV virions.

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Selected References

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  1. Cashel M., Lazzarini R. A., Kalbacher B. An improved method for thin-layer chromatography of nucleotide mixtures containing 32P-labelled orthophosphate. J Chromatogr. 1969 Mar 11;40(1):103–109. doi: 10.1016/s0021-9673(01)96624-5. [DOI] [PubMed] [Google Scholar]
  2. Ciardi J. E., Anderson E. P. Separation of purine and pyrimidine derivatives by thin-layer chromatography. Anal Biochem. 1968 Mar;22(3):398–408. doi: 10.1016/0003-2697(68)90282-0. [DOI] [PubMed] [Google Scholar]
  3. Cooper G. M., Temin H. M. Infectious rous sarcoma virus and reticuloendotheliosis virus DNAs. J Virol. 1974 Nov;14(5):1132–1141. doi: 10.1128/jvi.14.5.1132-1141.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dumas L. B., Darby G., Sinsheimer R. L. The replication of bacteriophage phi X174 DNA in vitro. Temperature effects on repair synthesis and displacement synthesis. Biochim Biophys Acta. 1971 Jan 28;228(2):407–422. [PubMed] [Google Scholar]
  5. Faras A. J., Levinson W. E., Bishop J. M., Goodman H. M. Identification of a tRNA nucleotidyltransferase and its substrates in virions of avian RNA tumor viruses. Virology. 1974 Mar;58(1):126–135. doi: 10.1016/0042-6822(74)90147-0. [DOI] [PubMed] [Google Scholar]
  6. Halpern M. S., Wade E., Rucker E., Baxter-Gabbard K. L., Levine A. S., Friis R. R. A study of the relationship of reticuloendotheliosis virus to the avian leukosis-sarcoma complex of viruses. Virology. 1973 Jun;53(2):287–299. doi: 10.1016/0042-6822(73)90206-7. [DOI] [PubMed] [Google Scholar]
  7. Ikemura T., Dahlberg J. E. Small ribonucleic acids of Escherichia coli. I. Characterization by polyacrylamide gel electrophoresis and fingerprint analysis. J Biol Chem. 1973 Jul 25;248(14):5024–5032. [PubMed] [Google Scholar]
  8. Kang C. Y. Characterization of endogenous RNA-directed DNA polymerase activity of reticuloendotheliosis viruses. J Virol. 1975 Oct;16(4):880–886. doi: 10.1128/jvi.16.4.880-886.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kang C. Y., Temin H. M. Reticuloendotheliosis virus nucleic acid sequences in cellular DNA. J Virol. 1974 Nov;14(5):1179–1188. doi: 10.1128/jvi.14.5.1179-1188.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mizutani A., Temin H. M. Purification and properties of spleen necrosis virus DNA polymerase. J Virol. 1975 Oct;16(4):797–806. doi: 10.1128/jvi.16.4.797-806.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mizutani S., Kang C. Y., Temin H. M. Endogenous RNA-directed DNA polymerase activity in virions of RNA tumor viruses and in a fraction from normal chicken cells. Methods Enzymol. 1974;29:119–124. doi: 10.1016/0076-6879(74)29014-1. [DOI] [PubMed] [Google Scholar]
  12. Mizutani S., Temin H. M. Lack of serological relationship among DNA polymerases of avian leukosis-sarcoma viruses, reticuloendotheliosis viruses, and chicken cells. J Virol. 1973 Sep;12(3):440–448. doi: 10.1128/jvi.12.3.440-448.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mizutani S., Temin H. M. Specific serological relationships among partially purified DNA polymerases of avian leukosis-sarcoma viruses, reticuloendotheliosis viruses, and avian cells. J Virol. 1974 May;13(5):1020–1029. doi: 10.1128/jvi.13.5.1020-1029.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mosser A. G. Polypeptide composition of spleen necrosis virus, a reticuloendotheliosis virus. J Virol. 1975 May;15(5):1088–1095. doi: 10.1128/jvi.15.5.1088-1095.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Randerath K., Randerath E. Ion-exchange thin-layer chromatography. XIV. Separation of nucleotide sugars and nucleoside monophosphates on PEI-cellulose. Anal Biochem. 1965 Dec;13(3):575–579. doi: 10.1016/0003-2697(65)90356-8. [DOI] [PubMed] [Google Scholar]
  16. Sarma P. S., Jain D. K., Mishra N. K., Vernon M. L., Paul P. S., Pomeroy B. S. Isolation and characterization of viruses from natural outbreaks of reticuloendotheliosis in turkeys. J Natl Cancer Inst. 1975 Jun;54(6):1355–1359. doi: 10.1093/jnci/54.6.1355. [DOI] [PubMed] [Google Scholar]
  17. Simpson R. W., Iinuma M. Recovery of infectious proviral DNA from mammalian cells infected with respiratory syncytial virus. Proc Natl Acad Sci U S A. 1975 Aug;72(8):3230–3234. doi: 10.1073/pnas.72.8.3230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Strauss J. H., Jr, Kelly R. B., Sinsheimer R. L. Denaturation of RNA with dimethyl sulfoxide. Biopolymers. 1968 Jun;6(6):793–807. doi: 10.1002/bip.1968.360060604. [DOI] [PubMed] [Google Scholar]
  19. Temin H. M., Kassner V. K. Replication of reticuloendotheliosis viruses in cell culture: acute infection. J Virol. 1974 Feb;13(2):291–297. doi: 10.1128/jvi.13.2.291-297.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Temin H. M. The cellular and molecular biology of RNA tumor viruses, especially avian leukosis-sarcoma viruses, and their relatives. Adv Cancer Res. 1974;19(0):47–104. doi: 10.1016/s0065-230x(08)60052-4. [DOI] [PubMed] [Google Scholar]
  21. Waite M. R., Allen P. T. RNA-directed DNA polymerase activity of reticuloendotheliosis virus: characterization of the endogenous and exogenous reactions. J Virol. 1975 Oct;16(4):872–879. doi: 10.1128/jvi.16.4.872-879.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Zhdanov V. M., Bogomolova N. N., Gavrilov V. I., Andzhaparidze O. G., Deryabin P. G., Astakhova A. N. Infectious DNA of tick-borne encephalitis virus. Arch Gesamte Virusforsch. 1974;45(3):215–224. doi: 10.1007/BF01249684. [DOI] [PubMed] [Google Scholar]
  23. Zhdanov V. M. Integration of viral genomes. Nature. 1975 Aug 7;256(5517):471–473. doi: 10.1038/256471a0. [DOI] [PubMed] [Google Scholar]
  24. Zhdanov V. M., Parfanovich M. I. Integration of measles virus nucleic acid into the cell genome. Arch Gesamte Virusforsch. 1974;45(3):225–234. doi: 10.1007/BF01249685. [DOI] [PubMed] [Google Scholar]

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