Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1976 Sep;19(3):871–878. doi: 10.1128/jvi.19.3.871-878.1976

Glycosylation sites of vesicular stomatitis virus glycoprotein.

J S Robertson, J R Etchison, D F Summers
PMCID: PMC354927  PMID: 184302

Abstract

Detailed analysis on DEAE-Sephadex of the tryptic digestion products of the glycoprotein from vesicular stomatitis virus grown in HeLa suspension cultures revealed the presence of two major and several minor sugar-labeled species. The minor tryptic glycopeptides were converted to one of the two major glycopeptide species by treatment with neuraminidase. Thus, vesicular stomatitis virus glycoprotein contains only two oligosaccharide side chains that are heterogeneous in their sialic acid content.

Full text

PDF
871

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkinson P. H. Synthesis and assembly of HeLa cell plasma membrane glycoproteins and proteins. J Biol Chem. 1975 Mar 25;250(6):2123–2134. [PubMed] [Google Scholar]
  2. Both G. W., Moyer S. A., Banerjee A. K. Translation and identification of the mRNA species synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1975 Jan;72(1):274–278. doi: 10.1073/pnas.72.1.274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Breindl M., Holland J. J. Coupled in vitro transcription and translation of vesicular stomatitis virus messenger RNA. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2545–2549. doi: 10.1073/pnas.72.7.2545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burge B. W., Huang A. S. Comparison of membrane protein glycopeptides of Sindbis virus and vesicular stomatitis virus. J Virol. 1970 Aug;6(2):176–182. doi: 10.1128/jvi.6.2.176-182.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Etchison J. R., Holland J. J. Carbohydrate composition of the membrane glycoprotein of vesicular stomatitis virus grown in four mammalian cell lines. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4011–4014. doi: 10.1073/pnas.71.10.4011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Grubman M. J., Summers D. F. In vitro protein-synthesizing activity of vesicular stomatitis virus-infected cell extracts. J Virol. 1973 Aug;12(2):265–274. doi: 10.1128/jvi.12.2.265-274.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HOWATSON A. F., WHITMORE G. F. The development and structure of vesicular stomatitis virus. Virology. 1962 Apr;16:466–478. doi: 10.1016/0042-6822(62)90228-3. [DOI] [PubMed] [Google Scholar]
  8. Keegstra K., Sefton B., Burke D. Sindbis virus glycoproteins: effect of the host cell on the oligosaccharides. J Virol. 1975 Sep;16(3):613–620. doi: 10.1128/jvi.16.3.613-620.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kelley J. M., Emerson S. U., Wagner R. R. The glycoprotein of vesicular stomatitis virus is the antigen that gives rise to and reacts with neutralizing antibody. J Virol. 1972 Dec;10(6):1231–1235. doi: 10.1128/jvi.10.6.1231-1235.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Koide N., Muramatsu T. Endo-beta-N-acetylglucosaminidase acting on carbohydrate moieties of glycoproteins. Purification and properties of the enzyme from Diplococcus pneumoniae. J Biol Chem. 1974 Aug 10;249(15):4897–4904. [PubMed] [Google Scholar]
  11. Moyer S. A., Summers D. F. Vesicular stomatitis virus envelope glycoprotein alterations induced by host cell transformation. Cell. 1974 May;2(1):63–70. doi: 10.1016/0092-8674(74)90009-9. [DOI] [PubMed] [Google Scholar]
  12. Moyer S. A., Tsang J. M., Atkinson P. H., Summers D. F. Oligosaccharide moieties of the glycoprotein of vesicular stomatitis virus. J Virol. 1976 Apr;18(1):167–175. doi: 10.1128/jvi.18.1.167-175.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mudd J. A., Summers D. F. Protein synthesis in vesicular stomatitis virus-infected HeLa cells. Virology. 1970 Oct;42(2):328–340. doi: 10.1016/0042-6822(70)90277-1. [DOI] [PubMed] [Google Scholar]
  14. WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]
  15. Wagner R. R., Prevec L., Brown F., Summers D. F., Sokol F., MacLeod R. Classification of rhabdovirus proteins: a proposal. J Virol. 1972 Dec;10(6):1228–1230. doi: 10.1128/jvi.10.6.1228-1230.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES