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. 1976 Sep;19(3):890–898. doi: 10.1128/jvi.19.3.890-898.1976

Natural immunity in mice to structural polypeptides of endogenous type C RNA viruses.

J R Stephenson, R L Peters, S Hino, R M Donahoe, L K Long, S A Aaronson, G J Kelloff
PMCID: PMC354929  PMID: 184303

Abstract

The immunological responses of inbred mice to structural components of one class of endogenous virus were investigated by means of radioimmunoassays utilizing highly purified viral proteins. Naturally occurring antiviral antibodies were demonstrated only in those strains possessing information for induction of a mouse cell-tropic endogenous virus. Moreover, these antibodies invariably appeared subsequent to the detection of spontaneous replication of this virus in the same animal. The immune responses elicited were much stronger against endogenous viral gp70 than p30, consistent with previous findings of tolerance in the mouse to the major structural antigen of its endogenous virus. However, the demonstration of an immune response to p30 under conditions of both natural and experimental immunization establishes that tolerance to this viral antigen can be overcome.

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Selected References

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  1. Aaronson S. A., Hartley J. W., Todaro G. J. Mouse leukemia virus: "spontaneous" release by mouse embryo cells after long-term in vitro cultivation. Proc Natl Acad Sci U S A. 1969 Sep;64(1):87–94. doi: 10.1073/pnas.64.1.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aaronson S. A., Stephenson J. R. Independent segregation of loci for activation of biologically distinguishable RNA C-type viruses in mouse cells. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2055–2058. doi: 10.1073/pnas.70.7.2055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Aaronson S. A., Stephenson J. R. Widespread natural occurrence of high titers of neutralizing antibodies to a specific class of endogenous mouse type-C virus. Proc Natl Acad Sci U S A. 1974 May;71(5):1957–1961. doi: 10.1073/pnas.71.5.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dougherty R. M., Di Stefano H. S., Roth F. K. Virus particles and viral antigens in chicken tissues free of infectious avian leukosis virus. Proc Natl Acad Sci U S A. 1967 Sep;58(3):808–817. doi: 10.1073/pnas.58.3.808. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hartley J. W., Rowe W. P. Clonal cells lines from a feral mouse embryo which lack host-range restrictions for murine leukemia viruses. Virology. 1975 May;65(1):128–134. doi: 10.1016/0042-6822(75)90013-6. [DOI] [PubMed] [Google Scholar]
  7. Hino S., Stephenson J. R., Aaronson S. A. Radiommunoassays for the 70,000-molecular-weight glycoproteins of endogenous mouse type C viruses: viral antigen expression in normal mouse tissues and sera. J Virol. 1976 Jun;18(3):933–941. doi: 10.1128/jvi.18.3.933-941.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Huebner R. J., Kelloff G. J., Sarma P. S., Lane W. T., Turner H. C., Gilden R. V., Oroszlan S., Meier H., Myers D. D., Peters R. L. Group-specific antigen expression during embryogenesis of the genome of the C-type RNA tumor virus: implications for ontogenesis and oncogenesis. Proc Natl Acad Sci U S A. 1970 Sep;67(1):366–376. doi: 10.1073/pnas.67.1.366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ihle J. N., Domotor J. J., Jr, Bengali K. M. Characterization of the type and group specificities of the immune response in mice to murine leukemia viruses. J Virol. 1976 Apr;18(1):124–131. doi: 10.1128/jvi.18.1.124-131.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ihle J. N., Hanna M. G., Jr, Roberson L. E., Kenney F. T. Autogenous immunity to endogenous RNA tumor virus. Identification of antibody reactivity to select viral antigens. J Exp Med. 1974 Jun 1;139(6):1568–1581. doi: 10.1084/jem.139.6.1568. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kelloff G. J., Peters R. L., Donahoe R. M., Ghazzouli I., Sass B., Nims R. M., Huebner R. J. An approach to C-type virus immunoprevention of spontaneously occurring tumors in laboratory mice. Cancer Res. 1976 Feb;36(2 Pt 2):622–630. [PubMed] [Google Scholar]
  12. Kelloff G., Vogt P. K. Localization of avian tumor virus group-specific antigen in cell and virus. Virology. 1966 Jul;29(3):377–384. doi: 10.1016/0042-6822(66)90213-3. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Levy J. A., Ihle J. N., Oleszko O., Barnes R. D. Virus-specific neutralization by a soluble non-immunoglobulin factor found naturally in normal mouse sera. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5071–5075. doi: 10.1073/pnas.72.12.5071. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lilly F., Duran-Reynals M. L., Rowe W. P. Correlation of early murine leukemia virus titer and H-2 type with spontaneous leukemia in mice of the BALB/c times AKR cross: a genetic analysis. J Exp Med. 1975 Apr 1;141(4):882–889. [PMC free article] [PubMed] [Google Scholar]
  16. Nowinski R. C., Kaehler S. L. Antibody to leukemia virus: widespread occurrence in inbred mice. Science. 1974 Sep 6;185(4154):869–871. doi: 10.1126/science.185.4154.869. [DOI] [PubMed] [Google Scholar]
  17. Oldstone M. B., Aoki T., Dixon F. J. The antibody response of mice to murine leukemia virus in spontaneous infection: absence of classical immunologic tolerance (AKR mice-complement-fixing antibodies-lymphocytic choriomeningitis virus-immunofluorescence-glomerular deposits of antigen-antibody complexes). Proc Natl Acad Sci U S A. 1972 Jan;69(1):134–138. doi: 10.1073/pnas.69.1.134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Peters R. L., Donahoe R. M., Kelloff G. J. Assay in the mouse for delayed-type hypersensitivity to murine leukemia virus. J Natl Cancer Inst. 1975 Nov;55(5):1089–1095. doi: 10.1093/jnci/55.5.1089. [DOI] [PubMed] [Google Scholar]
  19. Peters R. L., Spahn G. J., Rabstein L. S., Kelloff G. J., Huebner R. J. Oncogenic potential of murine C-type RNA virus passaged directly from naturally occurring tumors of the BALB-cCr mouse. J Natl Cancer Inst. 1973 Aug;51(2):621–630. [PubMed] [Google Scholar]
  20. Stephension J. R., Reynolds R. K., Tronick S. R., Aaronson S. A. Distribution of three classes of endogenous type-C RNA viruses among inbred strains of mice. Virology. 1975 Oct;67(2):404–414. doi: 10.1016/0042-6822(75)90442-0. [DOI] [PubMed] [Google Scholar]
  21. Stephenson J. R., Aaronson S. A. A genetic locus for inducibility of C-type in BALB-c cells: the effect of a nonlinked regulatory gene on detection of virus after chemical activation. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2798–2801. doi: 10.1073/pnas.69.10.2798. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Stephenson J. R., Aaronson S. A. Induction of an endogenous B-tropic type C RNA virus from SWR/J mouse embryo cells in tissue culture. Virology. 1976 Apr;70(2):352–359. doi: 10.1016/0042-6822(76)90277-4. [DOI] [PubMed] [Google Scholar]
  23. Stephenson J. R., Tronick S. R., Aaronson S. A. Isolation from BALB/c mouse cells of a structural polypeptide of a third endogenous type C virus. Cell. 1974 Dec;3(4):347–353. doi: 10.1016/0092-8674(74)90049-x. [DOI] [PubMed] [Google Scholar]
  24. Stephenson J. R., Wilsnack R. E., Aaronson S. A. Radioimmunoassay for avian C-type virus group-specific antigen: detection in normal and virus-transformed cells. J Virol. 1973 Jun;11(6):893–899. doi: 10.1128/jvi.11.6.893-899.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Strand M., August J. T. Structural proteins of mammalian oncogenic RNA viruses: multiple antigenic determinants of the major internal protein and envelope glycoprotein. J Virol. 1974 Jan;13(1):171–180. doi: 10.1128/jvi.13.1.171-180.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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