Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1976 Sep;19(3):1107–1110. doi: 10.1128/jvi.19.3.1107-1110.1976

Assay for type C virus in mouse sera based on particulate reverse transcriptase activity.

P Roy-Burman, M Dougherty, B K Pal, H P Charman, V Klement, M B Gardner
PMCID: PMC354951  PMID: 61284

Abstract

Assay of particulate reverse transcriptase activity in the sera from feral mice naturally infected with type C virus provides a sensitive and rapid procedure for the determination of in vivo virus infection. The results compare well with assays for infectious virus and with complement fixation or competitive radio-immunoassays for the p30 internal antigen of the virus.

Full text

PDF
1107

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore D. RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature. 1970 Jun 27;226(5252):1209–1211. doi: 10.1038/2261209a0. [DOI] [PubMed] [Google Scholar]
  2. Baltimore D., Smoler D. Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1507–1511. doi: 10.1073/pnas.68.7.1507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Charman H. P., Kim N., Gilden R. V. Radioimmunoassay for the major structural protein of hamster type C viruses. J Virol. 1974 Oct;14(4):910–917. doi: 10.1128/jvi.14.4.910-917.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fan H., Baltimore D. RNA metabolism of murine leukemia virus: detection of virus-specific RNA sequences in infected and uninfected cells and identification of virus-specific messenger RNA. J Mol Biol. 1973 Oct 15;80(1):93–117. doi: 10.1016/0022-2836(73)90235-0. [DOI] [PubMed] [Google Scholar]
  5. Gardner M. B., Henderson B. E., Estes J. D., Rongey R. W., Casagrande J., Pike M., Huebner R. J. The epidemiology and virology of C-type virus-associated hematological cancers and related diseases in wild mice. Cancer Res. 1976 Feb;36(2 Pt 2):574–581. [PubMed] [Google Scholar]
  6. Gardner M. B., Henderson B. E., Officer J. E., Rongey R. W., Parker J. C., Oliver C., Estes J. D., Huebner R. J. A spontaneous lower motor neuron disease apparently caused by indigenous type-C RNA virus in wild mice. J Natl Cancer Inst. 1973 Oct;51(4):1243–1254. doi: 10.1093/jnci/51.4.1243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gilden R. V., Oroszlan S., Huebner R. J. Coexistence of intraspecies and interspecies specific antigenic determinants on the major structural polypeptide of mammalian C-type viruses. Nat New Biol. 1971 May 26;231(21):107–108. doi: 10.1038/newbio231107a0. [DOI] [PubMed] [Google Scholar]
  8. Goodman N. C., Spiegelman S. Distinguishing reverse transcriptase of an RNA tumor virus from other known DNA polymerases. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2203–2206. doi: 10.1073/pnas.68.9.2203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hartley J. W., Rowe W. P., Capps W. I., Huebner R. J. Complement fixation and tissue culture assays for mouse leukemia viruses. Proc Natl Acad Sci U S A. 1965 May;53(5):931–938. doi: 10.1073/pnas.53.5.931. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hartley J. W., Rowe W. P. Clonal cells lines from a feral mouse embryo which lack host-range restrictions for murine leukemia viruses. Virology. 1975 May;65(1):128–134. doi: 10.1016/0042-6822(75)90013-6. [DOI] [PubMed] [Google Scholar]
  11. Pal B. K., Wright M., Officer J. E., Gardner M. B., Roy-Burman P. Subviral components of a wild mouse embryo-derived type C oncornavirus. Virology. 1973 Nov;56(1):189–197. doi: 10.1016/0042-6822(73)90298-5. [DOI] [PubMed] [Google Scholar]
  12. Robert M. S., Smith R. G., Gallo R. C., Sarin P. S., Abrell J. W. Viral and cellular DNA polymerase: comparison of activities with synthetic and natural RNA templates. Science. 1972 May 19;176(4036):798–800. doi: 10.1126/science.176.4036.798. [DOI] [PubMed] [Google Scholar]
  13. Roy-Burman P., Pal B. K., Kaplan M. B., Wright M., Gardner M. B. Biochemical properties of wild mouse oncornaviruses with lymphomagenic and neurotropic activities. Bibl Haematol. 1975;(40):627–637. doi: 10.1159/000397584. [DOI] [PubMed] [Google Scholar]
  14. Temin H. M., Mizutani S. RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature. 1970 Jun 27;226(5252):1211–1213. doi: 10.1038/2261211a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES