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. 1976 Oct;20(1):133–141. doi: 10.1128/jvi.20.1.133-141.1976

Polyoma genome in hamster BHK-21-C13 cells: integration into cellular DNA and induction of the viral replication.

W R Folk, J E Bancuk
PMCID: PMC354974  PMID: 185409

Abstract

When grown at 39.5 degrees C, BHK-21 C-13 cells transformed by A gene mutants of polyoma virus contain viral sequences that are predominantly associated with cellular DNA pelleted in the Hirt lysis procedure. At this temperature, in cells that are inducible for viral DNA replication (Folk, 1973), the majority of the viral genomes are covalently joined with cellular DNA's containing repetitious sequences. Upon a shift to 31 degrees C, free viral genomes appear and are replicated. Coupled with the replication of the free viral genomes at 31 degrees C is an increase in the viral genomes associated with cellular DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson D. M., Folk W. R. Iodination of DNA. Studies of the reaction and iodination of papovavirus DNA. Biochemistry. 1976 Mar 9;15(5):1022–1030. doi: 10.1021/bi00650a012. [DOI] [PubMed] [Google Scholar]
  2. Bellett A. J. Covalent integration of viral DNA into cell DNA in hamster cells transformed by an avian adenovirus. Virology. 1975 Jun;65(2):427–435. doi: 10.1016/0042-6822(75)90048-3. [DOI] [PubMed] [Google Scholar]
  3. Butel J. S., Brugge J. S., Noonan C. A. Transformation of primate and rodent cells by temperature-sensitive mutants of SV40. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):25–36. doi: 10.1101/sqb.1974.039.01.006. [DOI] [PubMed] [Google Scholar]
  4. Cheevers W. P., Hiscock J. B. DNA synthesis in polyoma virus infection. II. Relationship between viral DNA replication and initiation of cellular DNA replicons. J Mol Biol. 1973 Jun 25;78(1):237–245. doi: 10.1016/0022-2836(73)90441-5. [DOI] [PubMed] [Google Scholar]
  5. Cuzin F., Vogt M., Dieckmann M., Berg P. Induction of virus multiplication in 3T3 cells transformed by a thermosensitive mutant of polyoma virus. II. Formation of oligometric polyoma DNA molecules. J Mol Biol. 1970 Feb 14;47(3):317–333. doi: 10.1016/0022-2836(70)90305-0. [DOI] [PubMed] [Google Scholar]
  6. Eisen H., Pereira da Silva L., Jacob F. The regulation and mechanism of DNA synthesis in bacteriophage lambda. Cold Spring Harb Symp Quant Biol. 1968;33:755–764. doi: 10.1101/sqb.1968.033.01.086. [DOI] [PubMed] [Google Scholar]
  7. FRIED M. CELL-TRANSFORMING ABILITY OF A TEMPERATURE-SENSITIVE MUTANT OF POLYOMA VIRUS. Proc Natl Acad Sci U S A. 1965 Mar;53:486–491. doi: 10.1073/pnas.53.3.486. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fogel M. Induction of virus synthesis in polyoma-transformed cells by DNA antimetabolites and by irradiation after pretreatment with 5-bromodeoxyuridine. Virology. 1972 Jul;49(1):12–22. doi: 10.1016/s0042-6822(72)80003-5. [DOI] [PubMed] [Google Scholar]
  9. Folk W. R. Induction of virus synthesis in polyoma-transformed BHK-21 cells. J Virol. 1973 Mar;11(3):424–431. doi: 10.1128/jvi.11.3.424-431.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Folk W. R., Wang H. C. Closed circular DNAs with tandem repeats of a sequence from polyoma virus. Virology. 1974 Sep;61(1):140–155. doi: 10.1016/0042-6822(74)90249-9. [DOI] [PubMed] [Google Scholar]
  11. Francke B., Eckhart W. Polyoma gene function required for viral DNA synthesis. Virology. 1973 Sep;55(1):127–135. doi: 10.1016/s0042-6822(73)81014-1. [DOI] [PubMed] [Google Scholar]
  12. Gelb L. D., Kohne D. E., Martin M. A. Quantitation of Simian virus 40 sequences in African green monkey, mouse and virus-transformed cell genomes. J Mol Biol. 1971 Apr 14;57(1):129–145. doi: 10.1016/0022-2836(71)90123-9. [DOI] [PubMed] [Google Scholar]
  13. Gelb L. D., Martin M. A. Simian virus 40 DNA integration within the genome of virus-transformed mammalian cells. Virology. 1973 Feb;51(2):351–357. doi: 10.1016/0042-6822(73)90434-0. [DOI] [PubMed] [Google Scholar]
  14. Gross-Bellard M., Oudet P., Chambon P. Isolation of high-molecular-weight DNA from mammalian cells. Eur J Biochem. 1973 Jul 2;36(1):32–38. doi: 10.1111/j.1432-1033.1973.tb02881.x. [DOI] [PubMed] [Google Scholar]
  15. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  16. Kaplan J. C., Wilbert S. M., Black P. H. Analysis of simian virus 40-induced transformation of hamster kidney tissue in vitro. 8. Induction of infectious simian virus 40 from virogenie transformed hamster cells by amino acid deprivation or cycloheximide treatment. J Virol. 1972 Mar;9(3):448–453. doi: 10.1128/jvi.9.3.448-453.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kimura G., Itagaki A. Initiation and maintenance of cell transformation by simian virus 40: a viral genetic property. Proc Natl Acad Sci U S A. 1975 Feb;72(2):673–677. doi: 10.1073/pnas.72.2.673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kimura G. Temperature-sensitive growth of cells transformed by ts-a mutant of polyoma virus. Nature. 1975 Feb 20;253(5493):639–641. doi: 10.1038/253639b0. [DOI] [PubMed] [Google Scholar]
  19. Koprowski H., Jensen F. C., Steplewski Z. Activation of production of infectious tumor virus SV40 in heterokaryon cultures. Proc Natl Acad Sci U S A. 1967 Jul;58(1):127–133. doi: 10.1073/pnas.58.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Manor H., Fogel M., Sachs L. Integration of viral into chromosomal deoxyribonucleic acid in an inducible line of polyoma-transformed cells. Virology. 1973 May;53(1):174–185. doi: 10.1016/0042-6822(73)90476-5. [DOI] [PubMed] [Google Scholar]
  21. Martin R. G., Chou J. Y., Avila J., Saral R. The semiautonomous replicon: a molecular model for the oncogenicity of SV40. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):17–24. doi: 10.1101/sqb.1974.039.01.005. [DOI] [PubMed] [Google Scholar]
  22. Osborn M., Weber K. Simian virus 40 gene A function and maintenance of transformation. J Virol. 1975 Mar;15(3):636–644. doi: 10.1128/jvi.15.3.636-644.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Paulin D., Cuzin F. Polyoma virus T antigen. I. Synthesis of modified heat-labile T angiten in cells transformed with the ts-a mutant. J Virol. 1975 Feb;15(2):393–397. doi: 10.1128/jvi.15.2.393-397.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Reed S. I., Ferguson J., Davis R. W., Stark G. R. T antigen binds to simian virus 40 DNA at the origin of replication. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1605–1609. doi: 10.1073/pnas.72.4.1605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sambrook J., Westphal H., Srinivasan P. R., Dulbecco R. The integrated state of viral DNA in SV40-transformed cells. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1288–1295. doi: 10.1073/pnas.60.4.1288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tegtmeyer P. Function of simian virus 40 gene A in transforming infection. J Virol. 1975 Mar;15(3):613–618. doi: 10.1128/jvi.15.3.613-618.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Tegtmeyer P. Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon. J Virol. 1972 Oct;10(4):591–598. doi: 10.1128/jvi.10.4.591-598.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Varmus H. E., Vogt P. K., Bishop J. M. Integration of deoxyribonucleic acid specific for Rous sarcoma virus after infection of permissive and nonpermissive hosts. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3067–3071. doi: 10.1073/pnas.70.11.3067. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Vogt M. Induction of virus multiplication in 3T3 cells transformed by a thermosensitive mutant of polyoma virus. I. Isolation and characterization of Ts-a-3T3 cells. J Mol Biol. 1970 Feb 14;47(3):307–316. doi: 10.1016/0022-2836(70)90304-9. [DOI] [PubMed] [Google Scholar]
  30. Watkins J. F., Dulbecco R. Production of SV40 virus in heterokaryons of transformed and susceptible cells. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1396–1403. doi: 10.1073/pnas.58.4.1396. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Wetmur J. G., Davidson N. Kinetics of renaturation of DNA. J Mol Biol. 1968 Feb 14;31(3):349–370. doi: 10.1016/0022-2836(68)90414-2. [DOI] [PubMed] [Google Scholar]

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