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. 1976 Oct;20(1):203–210. doi: 10.1128/jvi.20.1.203-210.1976

Semliki Forest virus capsid protein associates with the 60S ribosomal subunit in infected cells.

I Ulmanen, H Söderlund, L Kääriäinen
PMCID: PMC354981  PMID: 824460

Abstract

Semlike forest virus capsid protein cosedimented with the large ribosomal subunit at 60S in sucrose gradients after treatment of cytoplasm from infected cells with Triton X-100 and EDTA. In CsCl gradients the capsid protein banded with the subunit at a density of 1.56 to 1.57 g/cm3. Most of the capsid protein could be detached from the 60S structure by treatment with 0.8 M KCl. The ribonucleoprotein of the 26S RNA had a sedimentation value of 53S and a density of 1.50 g/cm3 and could thus be separated from the 60S structure. The data suggest that the capsid protein binds to the large ribosomal subunit, but not to the viral 26S RNA.

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Selected References

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