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. 1976 Nov;20(2):373–383. doi: 10.1128/jvi.20.2.373-383.1976

Intracellular forms of adenovirus DNA. V. Viral DNA sequences in hamster cells abortively infected and transformed with human adenovirus type 12.

E Fanning, W Doerfler
PMCID: PMC355005  PMID: 988194

Abstract

The persistence of viral DNA in BHK-21 cells abortively infected with human adenovirus type 12 has been investigated using reassociation kinetics. No indication of an increase in the amount of viral DNA per cell has been found. On the contrary, the amount of intracellular viral DNA sequences decreases rapidly after infection. Thus, free adenovirus type 12 DNA does not replicate in BHK-21 cells. The influence of the multiplicity of infection on the amount of persisting adenovirus type 12 DNA has also been explored. The viral DNA sequences persisting in four lines of hamster cells transformed in vitro by adenovirus type 12 at various multiplicities of infection have been quantitated and mapped by reassociation kinetics experiments using restriction endonuclease fragments of 3H-labeled adenovirus type 12 DNA. All the EcoRI restriction nuclease fragments of the adenovirus type 12 genome are represented in each of the four cell lines. Individual fragments of the viral genome are represented in multiple copies in non-equimolar amounts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bellett A. J. Covalent integration of viral DNA into cell DNA in hamster cells transformed by an avian adenovirus. Virology. 1975 Jun;65(2):427–435. doi: 10.1016/0042-6822(75)90048-3. [DOI] [PubMed] [Google Scholar]
  3. Britten R. J., Kohne D. E. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. doi: 10.1126/science.161.3841.529. [DOI] [PubMed] [Google Scholar]
  4. Champe P. C., Strohl W. A., Schlesinger R. W. Characterization of the viral inhibitory factor (VIF) demonstrable in adenovirus-induced tumor and transformed cells. I. Effect on DNA and viral capsid protein synthesis. Virology. 1975 Dec;68(2):317–329. doi: 10.1016/0042-6822(75)90275-5. [DOI] [PubMed] [Google Scholar]
  5. Champe P. C., Strohl W. A., Schlesinger R. W. Demonstration of an adenovirus-inhibitory factor in adenovirus-induced hamster tumor cells. Virology. 1972 Nov;50(2):482–494. doi: 10.1016/0042-6822(72)90399-6. [DOI] [PubMed] [Google Scholar]
  6. Doerfler W., Burger H., Ortin J., Fanning E., Brown D. T., Mestphal M., Winterhoff U., Weiser B., Schick J. Integration of adenovirus DNA into the cellular genome. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):505–521. doi: 10.1101/sqb.1974.039.01.063. [DOI] [PubMed] [Google Scholar]
  7. Doerfler W. Integration of the deoxyribonucleic acid of adenovirus type 12 into the deoxyribonucleic acid of baby hamster kidney cells. J Virol. 1970 Nov;6(5):652–666. doi: 10.1128/jvi.6.5.652-666.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Doerfler W., Lundholm U. Absence of replication of the DNA of adenovirus type 12 in BHK21 cells. Virology. 1970 Mar;40(3):754–757. doi: 10.1016/0042-6822(70)90222-9. [DOI] [PubMed] [Google Scholar]
  9. Doerfler W. Nonproductive infection of baby hamster kidney cells (BHK21) with adenovirus type 12. Virology. 1969 Aug;38(4):587–606. doi: 10.1016/0042-6822(69)90179-2. [DOI] [PubMed] [Google Scholar]
  10. Doerfler W. The fate of the DNA of adenovirus type 12 in baby hamster kidney cells. Proc Natl Acad Sci U S A. 1968 Jun;60(2):636–643. doi: 10.1073/pnas.60.2.636. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  12. EAGLE H. Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc Soc Exp Biol Med. 1955 Jul;89(3):362–364. doi: 10.3181/00379727-89-21811. [DOI] [PubMed] [Google Scholar]
  13. Gallimore P. H. Viral DNA in transformed cells. II. A study of the sequences of adenovirus 2 DNA IN NINE LINES OF TRANSFORMED RAT CELLS USING SPECIFIC FRAGMENTS OF THE VIRAL GENOME;. J Mol Biol. 1974 Oct 15;89(1):49–72. doi: 10.1016/0022-2836(74)90162-4. [DOI] [PubMed] [Google Scholar]
  14. Gelb L. D., Kohne D. E., Martin M. A. Quantitation of Simian virus 40 sequences in African green monkey, mouse and virus-transformed cell genomes. J Mol Biol. 1971 Apr 14;57(1):129–145. doi: 10.1016/0022-2836(71)90123-9. [DOI] [PubMed] [Google Scholar]
  15. Graham F. L., Abrahams P. J., Mulder C., Heijneker H. L., Warnaar S. O., De Vries F. A., Fiers W., Van Der Eb A. J. Studies on in vitro transformation by DNA and DNA fragments of human adenoviruses and simian virus 40. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):637–650. doi: 10.1101/sqb.1974.039.01.077. [DOI] [PubMed] [Google Scholar]
  16. Graham F. L., van der Eb A. J., Heijneker H. L. Size and location of the transforming region in human adenovirus type 5 DNA. Nature. 1974 Oct 25;251(5477):687–691. doi: 10.1038/251687a0. [DOI] [PubMed] [Google Scholar]
  17. Green M. R., Chinnadurai G., Mackey J. K., Green M. A unique pattern of integrated viral genes in hamster cells transformed by highly oncogenic human adenovirus 12. Cell. 1976 Mar;7(3):419–428. doi: 10.1016/0092-8674(76)90172-0. [DOI] [PubMed] [Google Scholar]
  18. Green M. Effect of oncogenic DNA viruses on regulatory mechanisms of cells. Fed Proc. 1970 May-Jun;29(3):1265–1275. [PubMed] [Google Scholar]
  19. Green M. Molecular basis for the attack on cancer. Proc Natl Acad Sci U S A. 1972 Apr;69(4):1036–1041. doi: 10.1073/pnas.69.4.1036. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. HUEBNER R. J., ROWE W. P., LANE W. T. Oncogenic effects in hamsters of human adenovirus types 12 and 18. Proc Natl Acad Sci U S A. 1962 Dec 15;48:2051–2058. doi: 10.1073/pnas.48.12.2051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. MCBRIDE W. D., WIENER A. IN VITRO TRANSFORMATION OF HAMSTER KIDNEY CELLS BY HUMAN ADENOVIRUS TYPE 12. Proc Soc Exp Biol Med. 1964 Apr;115:870–874. doi: 10.3181/00379727-115-29060. [DOI] [PubMed] [Google Scholar]
  22. Mulder C., Sharp P. A., Delius H., Pettersson U. Specific fragmentation of DNA of adenovirus serotypes 3, 5, 7, and 12, and adeno-simian virus 40 hybrid virus Ad2+ND1 by restriction endonuclease R.EcoRI. J Virol. 1974 Jul;14(1):68–77. doi: 10.1128/jvi.14.1.68-77.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ortin J., Doerfler W. Transcription of the genome of adenovirus type 12. I. Viral mRNA in abortively infected and transformed cells. J Virol. 1975 Jan;15(1):27–35. doi: 10.1128/jvi.15.1.27-35.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ortin J., Scheidtmann K. H., Greenberg R., Westphal M., Doerfler W. Transcription of the genome of adenovirus type 12. III. Maps of stable RNA from productively infected human cells and abortively infected and transformed hamster cells. J Virol. 1976 Nov;20(2):355–372. doi: 10.1128/jvi.20.2.355-372.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. POPE J. H., ROWE W. P. IMMUNOFLUORESCENT STUDIES OF ADENOVIRUS 12 TUMORS AND OF CELLS TRANSFORMED OR INFECTED BY ADENOVIRUSES. J Exp Med. 1964 Oct 1;120:577–588. doi: 10.1084/jem.120.4.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Peacock A. C., Dingman C. W. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry. 1968 Feb;7(2):668–674. doi: 10.1021/bi00842a023. [DOI] [PubMed] [Google Scholar]
  27. Raska K., Jr, Strohl W. A. The response of BHK21 cells to infection with type 12 adenovirus. VI. Synthesis of virus-specific RNA. Virology. 1972 Mar;47(3):734–742. doi: 10.1016/0042-6822(72)90563-6. [DOI] [PubMed] [Google Scholar]
  28. STOKER M., MACPHERSON I. SYRIAN HAMSTER FIBROBLAST CELL LINE BHK21 AND ITS DERIVATIVES. Nature. 1964 Sep 26;203:1355–1357. doi: 10.1038/2031355a0. [DOI] [PubMed] [Google Scholar]
  29. Sambrook J., Botchan M., Gallimore P., Ozanne B., Pettersson U., Williams J., Sharp P. A. Viral DNA sequences in cells transformed by simian virus 40, adenovirus type 2 and adenovirus type 5. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):615–632. doi: 10.1101/sqb.1974.039.01.075. [DOI] [PubMed] [Google Scholar]
  30. Sharp P. A., Pettersson U., Sambrook J. Viral DNA in transformed cells. I. A study of the sequences of adenovirus 2 DNA in a line of transformed rat cells using specific fragments of the viral genome. J Mol Biol. 1974 Jul 15;86(4):709–726. doi: 10.1016/0022-2836(74)90348-9. [DOI] [PubMed] [Google Scholar]
  31. Strohl W. A., Rabson A. S., Rouse H. Adenovirus tumorigenesis: role of the viral genome in determining tumor morphology. Science. 1967 Jun 23;156(3782):1631–1633. doi: 10.1126/science.156.3782.1631. [DOI] [PubMed] [Google Scholar]
  32. Strohl W. A., Rouse H., Teets K., Schlesinger R. W. The response of BHK21 cells to infection with type 12 adenovirus. 3. Transformation and restricted replication of superinfecting type 2 adenovirus. Arch Gesamte Virusforsch. 1970;31(1):93–112. doi: 10.1007/BF01241669. [DOI] [PubMed] [Google Scholar]
  33. Strohl W. A. The response of BHK21 cells to infection with type 12 adenovirus. 1. Cell killing and T antigen synthesis as correlated viral genome functions. Virology. 1969 Dec;39(4):642–652. doi: 10.1016/0042-6822(69)90003-8. [DOI] [PubMed] [Google Scholar]
  34. Strohl W. A. The response of BHK21 cells to infection with type 12 adenovirus. II. Relationship of virus-stimulated DNA synthesis to other viral functions. Virology. 1969 Dec;39(4):653–665. doi: 10.1016/0042-6822(69)90004-x. [DOI] [PubMed] [Google Scholar]
  35. TRENTIN J. J., YABE Y., TAYLOR G. The quest for human cancer viruses. Science. 1962 Sep 14;137(3533):835–841. doi: 10.1126/science.137.3533.835. [DOI] [PubMed] [Google Scholar]
  36. Vogt V. M. Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae. Eur J Biochem. 1973 Feb 15;33(1):192–200. doi: 10.1111/j.1432-1033.1973.tb02669.x. [DOI] [PubMed] [Google Scholar]
  37. Wetmur J. G., Davidson N. Kinetics of renaturation of DNA. J Mol Biol. 1968 Feb 14;31(3):349–370. doi: 10.1016/0022-2836(68)90414-2. [DOI] [PubMed] [Google Scholar]
  38. Zur Hausen H. Association of adenovirus type 12 deoxyribonucleic acid with host cell chromosomes. J Virol. 1968 Mar;2(3):218–223. doi: 10.1128/jvi.2.3.218-223.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. zur Hausen H. Interactions of adenovirus type 12 with host cell chromosomes. Prog Exp Tumor Res. 1973;18:240–259. doi: 10.1159/000393169. [DOI] [PubMed] [Google Scholar]

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