Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1976 Nov;20(2):509–519. doi: 10.1128/jvi.20.2.509-519.1976

New temperate bacteriophage for Bacillus subtilis, rho 11.

D H Dean, J C Orrego, K W Hutchison, H O Halvorson
PMCID: PMC355018  PMID: 62060

Abstract

A new temperate bacteriophage, rho11, isolated by J. Hoch, has been characterized. This new phage is very similar to the temperate phage phi3T in size (380 nm), host range, homoimmunity, DNA buoyant density (1.694 g/ml), antigenicity, and molecular weight (around 6.0 X 10(7)) as determined in gels. Like phi3T, rho11 converts thymine auxotrophs to prototrophy at high frequency (250 out of 250 tested). Phage rho11 differs from phi3T in plaque morphology and in the endonuclease R-EcoRI digest pattern. Sixteen of the 20 rho11 DNA fragments have migration patterns corresponding to those of the 21 fragments of phi3T. The close similarities yet clear differences between these phages suggest that the two phages have a common ancestor.

Full text

PDF
509

Images in this article

514Image
on p.514
515Image
on p.515
517Image
on p.517

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOTT K., STRAUSS B. THE CARRIER STATE OF BACILLUS SUBTILIS INFECTED WITH THE TRANSDUCING BACTERIOPHAGE SP10. Virology. 1965 Feb;25:212–225. doi: 10.1016/0042-6822(65)90200-x. [DOI] [PubMed] [Google Scholar]
  2. Beckwith J. R., Signer E. R., Epstein W. Transposition of the Lac region of E. coli. Cold Spring Harb Symp Quant Biol. 1966;31:393–401. doi: 10.1101/sqb.1966.031.01.051. [DOI] [PubMed] [Google Scholar]
  3. Birdsell D. C., Hathaway G. M., Rutberg L. Characterization of Temperate Bacillus Bacteriophage phi105. J Virol. 1969 Sep;4(3):264–270. doi: 10.1128/jvi.4.3.264-270.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boice L. B. Evidence that Bacillus subtilis bacteriophage SP02 is temperate and heteroimmune to bacteriophage phi-105. J Virol. 1969 Jul;4(1):47–49. doi: 10.1128/jvi.4.1.47-49.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dubnau D., Davidoff-Abelson R., Smith I. Transformation and transduction in Bacillus subtilis: evidence for separate modes of recombinant formation. J Mol Biol. 1969 Oct 28;45(2):155–179. doi: 10.1016/0022-2836(69)90097-7. [DOI] [PubMed] [Google Scholar]
  6. Harris-Warrick R. M., Elkana Y., Ehrlich S. D., Lederberg J. Electrophoretic separation of Bacillus subtilis genes. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2207–2211. doi: 10.1073/pnas.72.6.2207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Helling R. B., Goodman H. M., Boyer H. W. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. J Virol. 1974 Nov;14(5):1235–1244. doi: 10.1128/jvi.14.5.1235-1244.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. JACOB F., MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318–356. doi: 10.1016/s0022-2836(61)80072-7. [DOI] [PubMed] [Google Scholar]
  9. JACOB F., WOLLMAN E. Induction spontanée du développement du bactériophage lambda au cours de la recombinaison génétique, chez Escherichia coli K 12. C R Hebd Seances Acad Sci. 1954 Jul 19;239(3):317–319. [PubMed] [Google Scholar]
  10. Morse M L, Lederberg E M, Lederberg J. Transduction in Escherichia Coli K-12. Genetics. 1956 Jan;41(1):142–156. doi: 10.1093/genetics/41.1.142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Morse M L, Lederberg E M, Lederberg J. Transductional Heterogenotes in Escherichia Coli. Genetics. 1956 Sep;41(5):758–779. doi: 10.1093/genetics/41.5.758. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Murray N. E., Murray K. Manipulation of restriction targets in phage lambda to form receptor chromosomes for DNA fragments. Nature. 1974 Oct 11;251(5475):476–481. doi: 10.1038/251476a0. [DOI] [PubMed] [Google Scholar]
  13. Rambach A., Tiollais P. Bacteriophage lambda having EcoRI endonuclease sites only in the nonessential region of the genome. Proc Natl Acad Sci U S A. 1974 Oct;71(10):3927–3930. doi: 10.1073/pnas.71.10.3927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Romig W. R. Infectivity of Bacillus subtilis bacteriophage deoxyribonucleic acids extracted from mature particles and from lysogenic hosts. Bacteriol Rev. 1968 Dec;32(4 Pt 1):349–357. [PMC free article] [PubMed] [Google Scholar]
  15. Rutberg L. Mapping of a temperate bacteriophage active on Bacillus subtilis. J Virol. 1969 Jan;3(1):38–44. doi: 10.1128/jvi.3.1.38-44.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SCHILDKRAUT C. L., MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962 Jun;4:430–443. doi: 10.1016/s0022-2836(62)80100-4. [DOI] [PubMed] [Google Scholar]
  17. Shapiro J. A., Dean D. H., Halvorson H. O. Low-frequency specialized transduction with Bacillus subtilis bacteriophage phi 105. Virology. 1974 Dec;62(2):393–403. doi: 10.1016/0042-6822(74)90401-2. [DOI] [PubMed] [Google Scholar]
  18. THORNE C. B. Transduction in Bacillus subtilis. J Bacteriol. 1962 Jan;83:106–111. doi: 10.1128/jb.83.1.106-111.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Thomas M., Cameron J. R., Davis R. W. Viable molecular hybrids of bacteriophage lambda and eukaryotic DNA. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4579–4583. doi: 10.1073/pnas.71.11.4579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tucker R. G. Acquisition of thymidylate synthetase activity by a thymine-requiring mutant of Bacillus subtilis following infection by the temperate phage phi 3. J Gen Virol. 1969 Jun;4(4):489–504. doi: 10.1099/0022-1317-4-4-489. [DOI] [PubMed] [Google Scholar]
  21. VINOGRAD J., HEARST J. E. Equilibrium sedimentation of macromolecules and viruses in a density gradient. Fortschr Chem Org Naturst. 1962;20:373–422. [PubMed] [Google Scholar]
  22. Wilson G. A., Williams M. T., Baney H. W., Young F. E. Characterization of temperate bacteriophages of Bacillus subtilis by the restriction endonuclease EcoRI: evidence for three different temperate bacteriophages. J Virol. 1974 Oct;14(4):1013–1016. doi: 10.1128/jvi.14.4.1013-1016.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Yehle C. O., Doi R. H. Differential expression of bacteriophage genomes in vegetative and sporulating cells of Bacillus subtilis. J Virol. 1967 Oct;1(5):935–947. doi: 10.1128/jvi.1.5.935-947.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Zubay G., Chambers D. A. A DNA-directed cell-free system for beta-galactosidase synthesis; characterization of the de novo synthesized enzyme and some aspects of the regulation of synthesis. Cold Spring Harb Symp Quant Biol. 1969;34:753–761. doi: 10.1101/sqb.1969.034.01.085. [DOI] [PubMed] [Google Scholar]
  25. Zubay G., Morse D. E., Schrenk W. J., Miller J. H. Detection and isolation of the repressor protein for the tryptophan operon of Escherichia coli. Proc Natl Acad Sci U S A. 1972 May;69(5):1100–1103. doi: 10.1073/pnas.69.5.1100. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES