Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1976 Nov;20(2):535–538. doi: 10.1128/jvi.20.2.535-538.1976

Bacteriophage PBS2-induced inhibition of uracil-containing DNA degradation.

G E Katz, A R Price, M J Pomerantz
PMCID: PMC355022  PMID: 824463

Abstract

Degradation of uracil-containing DNA by Bacillus subtilis extracts and its inhibition after infection by the uracil-containing DNA phage PBS2 have been investigated to resolve differences between the published reports of Tomita and Takahashi (1975) and Friedberg et al. (1975, 1976). The product of hydrolysis of PBS2 DNA, tritium labeled in its uracil and cytosine residues, is solely uracil and not deoxyuridine. The degrading activity is completely inhibited within 7 min after PBS2 infection, before any other known PBS2-induced protein is detectable. The production of the PBS2 inhibitor (a small, heat-stable protein) continues until 10 to 20 min postinfection.

Full text

PDF
535

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clark S., Losick R., Pero J. New RNA polymerase from Bacillus subtilis infected with phage PBS2. Nature. 1974 Nov 1;252(5478):21–24. doi: 10.1038/252021a0. [DOI] [PubMed] [Google Scholar]
  2. Duncan J., Hamilton L., Friedberg E. C. Enzymatic degradation of uracil-containing DNA. II. Evidence for N-glycosidase and nuclease activities in unfractionated extracts of Bacillus subtilis. J Virol. 1976 Aug;19(2):338–345. doi: 10.1128/jvi.19.2.338-345.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Friedberg E. C., Ganesan A. K., Minton K. N-Glycosidase activity in extracts of Bacillus subtilis and its inhibition after infection with bacteriophage PBS2. J Virol. 1975 Aug;16(2):315–321. doi: 10.1128/jvi.16.2.315-321.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Lindahl T. An N-glycosidase from Escherichia coli that releases free uracil from DNA containing deaminated cytosine residues. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3649–3653. doi: 10.1073/pnas.71.9.3649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lindahl T. New class of enzymes acting on damaged DNA. Nature. 1976 Jan 1;259(5538):64–66. doi: 10.1038/259064a0. [DOI] [PubMed] [Google Scholar]
  7. Price A. R. Bacteriophage PBS2-induced deoxycytidine triphosphate deaminase in Bacillus subtilis. J Virol. 1974 Nov;14(5):1314–1317. doi: 10.1128/jvi.14.5.1314-1317.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Price A. R., Cook S. J. New deoxyribonucleic acid polymerase induced by Bacillus subtilis bacteriophage PBS2. J Virol. 1972 Apr;9(4):602–610. doi: 10.1128/jvi.9.4.602-610.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Price A. R., Fogt S. M. Deoxythymidylate phosphohydrolase induced by bacteriophage PBS2 during infection of Bacillus subtilis. J Biol Chem. 1973 Feb 25;248(4):1372–1380. [PubMed] [Google Scholar]
  10. Price A. R., Frato J. Bacillus subtilis deoxyuridinetriphosphatase and its bacteriophage PBS2-induced inhibitor. J Biol Chem. 1975 Nov 25;250(22):8804–8811. [PubMed] [Google Scholar]
  11. Price A. R., Hitzeman R., Frato J., Lombardi K. Rifampicin-resistant bacteriophage PBS2 infection and RNA polymerase in Bacillus subtilis. Nucleic Acids Res. 1974 Nov;1(11):1497–1502. doi: 10.1093/nar/1.11.1497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. TAKAHASHI I., MARMUR J. Replacement of thymidylic acid by deoxyuridylic acid in the deoxyribonucleic acid of a transducing phage for Bacillus subtilis. Nature. 1963 Feb 23;197:794–795. doi: 10.1038/197794a0. [DOI] [PubMed] [Google Scholar]
  13. Tomita F. Changes in DNase activities in Bacillus subtilis infected with bacteriophage PBS 1. J Virol. 1975 May;15(5):1073–1080. doi: 10.1128/jvi.15.5.1073-1080.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tomita F., Takahashi I. A novel enzyme, dCTP deaminase, found in Bacillus subtilis infected with phage PBS I. Biochim Biophys Acta. 1969 Mar 18;179(1):18–27. doi: 10.1016/0005-2787(69)90117-8. [DOI] [PubMed] [Google Scholar]
  15. Tomita F., Takahashi I. DNase Specific for Uracil-Containing Bacteriophage DNA. J Virol. 1975 May;15(5):1081–1087. doi: 10.1128/jvi.15.5.1081-1087.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES