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. 2009 Oct 28;15(3):211–224. doi: 10.1007/s12298-009-0025-7

Analysis of diversity amongst widely distributed and endemic Atalantia (family Rutaceae) species from Western Ghats of India

S A Ranade 1,✉, K N Nair 1, Anuj P Srivastava 1, P Pushpangadan 1
PMCID: PMC3550358  PMID: 23572931

Abstract

The genus Atalantia Correa is represented in India with four species and two varieties. The infra — generic classification and the species limits in Atalantia are, however, not well defined, due to the occurrence of intermediate forms. Two species, A. racemosa Wight and A. monophylla (L.) DC., are widely distributed, while the third species, A. wightii Tanaka is endemic in the Western Ghats, a well-known biodiversity hotspot. PCR-based methods have been commonly used for the assessment of genetic diversity in plants. We report for the first time the genetic diversity within and between populations of the above three species using two Single Primer Amplification Reaction (SPAR) methods. AMOVA analysis clearly indicates the lesser diversity among the species than within them. The UPGMA tree included all of the doubtful species in one single sub-cluster within the major cluster of A. racemosa and A. wightii, suggesting that these are probably hybrids derived from these two species. At the population level, all A. monophylla populations grouped together in a cluster that was clearly separated from all other species and populations.

Key words: AMOVA, Atalantia, diversity, PCA, Rutaceae, SPAR methods, Western Ghats

Full Text

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Abbreviations

AMOVA

Analysis of Molecular Variation

DAMD

Directed Amplification of Minisatellite DNA

ISSR

Inter Simple Sequence Repeat

PCA

Principal Components Analysis

RAPD

Random Amplified Polymorphic DNA

SPAR

Single Primer Amplification Reaction

UPGMA

Unweighted Pair Group Method with Arithmetic Means

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