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. 2009 Jun 28;15(2):151–160. doi: 10.1007/s12298-009-0017-7

Differential expression of physiological and biochemical characters of some Indian mangroves towards salt tolerance

Paramita Nandy (Datta) 2, Nirjhar Dasgupta 1, Sauren Das 1,✉
PMCID: PMC3550367  PMID: 23572924

Abstract

Mangroves are physiologically interesting as potential models for stress tolerance and as sources of alternative ideas about physiological strategies relevant at the ecosystem level. Variation in habitat has great impact on the physiological behavior and biochemical expression level of a particular plant species. Five species of mangroves, growing in saline and fresh water conditions were assessed for their ecological fitness in two different habitats. Assessments were based on some physiological and biochemical parameters measured from the fully exposed mature leaves under saline (15–27 PPT) and non-saline (1.2–2 PPT) conditions. Among the five species considered for investigation Bruguiera gymnorrhiza, Excoecaria agallocha and Phoenix paludosa grow luxuriously in the Sundarbans forest, while the rest two (Heritiera fomes, Xylocarpus granatum) are scanty. A comparative account of photosynthetic efficiency, chlorophyll content, mesophyll and stomatal conductance, specific leaf area, photosynthetic nitrogen use efficiency, total foliar free amino acids and differential expression of some antioxidant isoenzymes in leaf were estimated between the saline and non-saline plants. Elevated assimilation rate coupled with increased chlorophyll content, increased conductance and higher specific leaf area in non-saline condition indicates ability of these mangroves to grow even under minimal substrate salinity. The optimum PAR acquisition for photosynthesis in B. gymnorrhiza, E. agallocha and P. paludosa was higher under salt stress, while the maximum assimilation rate was lower in control plants. The opposite trend occurred in H. fomes and X. granatum, where the peak photosynthesis was lower under non-saline conditions even at a higher irradiance than in the saline forest. The isoform patterns of peroxidase, acid phosphatase and esterase indicated considerable difference in regulation of these enzymes due to salt stress and /or reverse adaptation.

Key words: Chlorophyll content, Isozymes, Mangroves, Photosynthetic efficiency, Specific leaf area

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References

  1. Ball M.C. Ecophysiology of mangroves. Trees: Structure and function. 1988;2:129–142. [Google Scholar]
  2. Bhosale LJ (1985) Free amino acids in mangroves-significance. Proc. Nat. Symp. Biol. Util. Cons. Mangroves, Kolhapur, 558.
  3. Bjorkman O., Demmig B., Andrews T.J. Mangrove Photosynthesis: Response to High-Irradiance Stress. Australian Journal of Plant Physiology. 1988;15(2):43–61. [Google Scholar]
  4. Bongi G., Loreto F. Gas-exchange properties of salt stressed olive (Olea europea L.) leaves. Plant Physiology. 1989;90:1408–1416. doi: 10.1104/pp.90.4.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brugnoli E., Bjorkman O. Growth of cotton under continuous salinity stress: Influence on allocation pattern, stomatal and non-stomatal components of photosynthesis and dissipation of excess light energy. Planta. 1992;187:338–347. doi: 10.1007/BF00195657. [DOI] [PubMed] [Google Scholar]
  6. Cheesman J.M., Lovelock C.E. Photosynthetic characteristics of dwarf and fringe Rhizophora mangle L. in Belizean mangrove. Plant Cell and Environment. 2004;27(6):769–780. doi: 10.1111/j.1365-3040.2004.01181.x. [DOI] [Google Scholar]
  7. Choudhury JK (1996) Mangrove forest management. Mangrove rehabilitation and management project in Sulawesi. p. 297.
  8. Chow W.S., Ball M.C., Anderson J.M. Growth and photosynthetic responses of spinach to salinity: implications of K+ nutrition for salt tolerance. Australian Journal of Plant Physiology. 1990;17:563–567. doi: 10.1071/PP9900563. [DOI] [Google Scholar]
  9. Clough B.F. Effect of nutrient supply on photosynthesis in mangroves. In: Bhosale L. J., editor. The mangroves; Kolhapur, India: Shivaji University; 1985. pp. 80–88. [Google Scholar]
  10. Cowan I.R. Water Relations and Carbon Assimilation. Physiological Plant Ecology. II. Berlin: Springer-Verlag; 1982. Regulation of water use in relation to carbon gain in higher plants; pp. 589–614. [Google Scholar]
  11. Das S. An adaptive feature of some mangroves of Sundarbans, West Bengal. Journal of Plant Biology. 1999;42(2):109–116. doi: 10.1007/BF03031018. [DOI] [Google Scholar]
  12. Demiral M.A., Aydin M., Yorulmaz A. Effect of salinity on growth chemical composition and antioxidative enzyme activity of two malting barley (Hordum vulgare L.) cultivars. Turkish Journal of Biology. 2005;29:117–123. [Google Scholar]
  13. Dijkstra P. Cause and effect of differences in specific leaf area. In: Lambers H. M., Cambridge L., Konings H., Pons T. L., editors. Cause and consequences of variation in growth rate and productivity of higher plants. The Netherlands: SPB Academic Publishing, The Hague; 1990. pp. 125–144. [Google Scholar]
  14. Feller I.C., Whigham D.F., McKee K.L., Loveloc C.E. Nitrogen limitation of growth and nutrient dynamics in a disturbed mangrove forest, Indian River Lagoon, Florida. Oceologia. 2003;134(3):405–414. doi: 10.1007/s00442-002-1117-z. [DOI] [PubMed] [Google Scholar]
  15. Flowers T.J., Troke P.F., Yeo A.R. The mechanism of salt tolerance in halophytes. Annu. Rev. Plant Physiol. 1977;28:89–121. doi: 10.1146/annurev.pp.28.060177.000513. [DOI] [Google Scholar]
  16. Frenkel C. Involvement of Peroxidase and Indole-3-acetic Acid Oxidase Isozymes from Pear, Tomato, and Blueberry Fruit in Ripening. Plant Physiology. 1972;49:757–763. doi: 10.1104/pp.49.5.757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Gangopadhyay G., Das S., Mukherjee K.K. Speciation in Chenopodium in west Bengal, India. Genetic Research of Crop Evolution. 2002;49:459–461. [Google Scholar]
  18. Grassi G., Meir P., Cormer R.N., Tompkins D., Jarvis P.G. Photosynthetic parameters in seedlings of Eucalyptus grandis as affected by rate of nitrogen supply. Plant Cell and Environment. 2002;25:1677–1688. doi: 10.1046/j.1365-3040.2002.00946.x. [DOI] [Google Scholar]
  19. Heldt H.W. Plant biochemistry and molecular biology. Oxford: Oxford University Press; 1999. pp. 247–276. [Google Scholar]
  20. James S.A., Smith W.K., Vogelmann E.C. Ontogenetic differences in mesophyll structure and chlorophyll distribution in Eucalyptus globulus ssp. globulus (Myrtaceae) American Journal of Botany. 1999;86:198–207. doi: 10.2307/2656937. [DOI] [PubMed] [Google Scholar]
  21. Joshi G.V., Dolan T., Gee R., Saltman P. Plant Physiol. 1962;37:446–449. doi: 10.1104/pp.37.3.446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Kathiresan K., Kannan L. Photosynthetic productivity in species of Rhizophora. In: Bhosale L. J., editor. The Mangroves; Kolhapur, India: Shivaji University; 1985. pp. 262–265. [Google Scholar]
  23. Kathiresan K., Moorthy P. Influence of different irradiance on growth and photosynthetic characteristics in seedlings of Rhizophora species. Photosynthetica. 1994;29:143–146. [Google Scholar]
  24. Kawasaki S., Borchert C., Deyholos M. Gene expression profiles during the initial phase of salt stress in rice. Plant Cell. 2001;13:889–905. doi: 10.1105/tpc.13.4.889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Khan M.A., Ungar I.A., Showalter A.M. The effect of salinity on the growth, water status and ion content of a leaf succulent perennial halophyte Sueada fruticosa (L.) Forssk. Journal of Aried. Environment. 2000;45:73–84. doi: 10.1006/jare.1999.0617. [DOI] [Google Scholar]
  26. Khan M.S. Flora of Bangladesh. Report 4. Camelinanceae. Farmgate, Dhaka, Bangladesh: Bangladesh National Herbarium, Bangladesh Agriculture Research Council (BARC); 1977. [Google Scholar]
  27. Kiato M., Utsugi H., Kuramoto S., Tabuchi R., Fujimoto K., Lihpal S. Lightdependent photosynthetic characteristics indicated by chlorophyll fluorescence in five mangrove species native to Pohnpei Island, Micronesia. Physiologia Plantarum. 2003;117(3):376–382. doi: 10.1034/j.1399-3054.2003.00042.x. [DOI] [PubMed] [Google Scholar]
  28. Krauss K.W., Allen J.A. Influence of salinity and shade on seedling photosynthesis and growth of two mangrove species Rhizophora mangle and Bruguiera sexangula, introduced to Hawaii. Aquatic Botany. 2003;77(4):311–324. doi: 10.1016/j.aquabot.2003.08.004. [DOI] [Google Scholar]
  29. Lichtenthaler H.K., Wellburn A.R. Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transaction. 1983;603:591–592. [Google Scholar]
  30. Loreto F., Harley P.C., Marco G.D., Sharkey T.D. Estimation of mesophyll conductance to CO2 flux by three different methods. Plant Physiology. 1992;98:1437–1443. doi: 10.1104/pp.98.4.1437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Mader M. Die Localization der Peroxidase Iso-. enzymgruppe G, in der Zellwand von Tabak-Geweben. Planta. 1976;131:11–15. doi: 10.1007/BF00387338. [DOI] [PubMed] [Google Scholar]
  32. Nandy Photosynthesis and water-use efficiency of some mangroves of Sundarbans, India. Journal of Plant Biology. 2001;44:213–219. doi: 10.1007/BF03030354. [DOI] [Google Scholar]
  33. Nandy Estimation of osmotic potential and free amino acids in some mangroves of the Sundarbans, India. Acta botanica Croetica. 2003;62(1):37–45. [Google Scholar]
  34. Nandy Photosynthesis and water-use characterististics in Indian mangroves. Journal of Plant Biology. 2005;48(2):245–252. doi: 10.1007/BF03030414. [DOI] [Google Scholar]
  35. Nandy Relation of leaf micromorphology with photosynthesis and water efflux in some Indian mangroves. Acta Botanica Croatica. 2005;64(2):331–340. [Google Scholar]
  36. Naidoo G., Tuffers A.V., von Willert D.J. Changes in gas exchange and chlorophyll fluorescence characteristics of two mangroves and a mangrove associate in response to salinity in the natural environment. Trees. 2002;16(2–3):140–146. [Google Scholar]
  37. Parida A.K., Das A.B., Mitra B. Effect of salt and growth, ion accumulation, photosynthesis and leaf anatomy of the mangrove. Bruguiera parviflora. Trees. 2004;18:167–174. [Google Scholar]
  38. Porra R.J., Thompson W.A., Kriedemann P.E. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic spectroscopy. Biochemistry Biophysics Acta. 1989;975:384–394. doi: 10.1016/S0005-2728(89)80347-0. [DOI] [Google Scholar]
  39. Poorter H., Evans J. Photosynthetic nitrogen use efficiency of species that differ inherently in specific leaf area. Oecologia. 1998;116:26–37. doi: 10.1007/s004420050560. [DOI] [PubMed] [Google Scholar]
  40. Schulze E.D., Caldwell M.M., Canadell J., Mooney H.A., Jackson R.B., Parson D., Scholes R., Sala O.E., Trimborn P. Downward flux of water through roots (i.e., inverse hydraulic lift) in dry Kalahari sands. Oecologia. 1998;115:460–462. doi: 10.1007/s004420050541. [DOI] [PubMed] [Google Scholar]
  41. Searson M.G., Thomas D.S., Montagu K.D., Conroy J.P. Leaf water use efficiency differs between Eucalyptus seedlings from contrasting rainfall environments. Functional Plant Biology. 2004;31:441–450. doi: 10.1071/FP03199. [DOI] [PubMed] [Google Scholar]
  42. Sefton C.A., Kelvin M., Atwell B.J., Conroy P.J. Anatomical variation in juvenile eucalypt leaves accounts for differences in specific leaf area and CO2 assimilation rates. Australian Journal of Plant Physiology. 2002;50(3):301–310. [Google Scholar]
  43. Stonier T., Yang H.-M. Studies on Auxin Protectors: XI. Inhibition of Peroxidase-Catalyzed Oxidation of Glutathione by Auxin Protectors and o Dihydroxyphenols. Plant Physiology. 1973;51:391–395. doi: 10.1104/pp.51.2.391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. Thornber J.P. Chlorophyll proteins light harvesting and reaction center components of plants. Annual Review of Plant Physiology. 1975;26:127–158. doi: 10.1146/annurev.pp.26.060175.001015. [DOI] [Google Scholar]
  45. Werner A., Stelzer R. Physiological responses of the mangrove Rhizophora mangle grown in the absence and presence of NaCl. Plant Cell Environment. 1990;13:243–255. doi: 10.1111/j.1365-3040.1990.tb01309.x. [DOI] [Google Scholar]
  46. Wright G.C., Nageswara Rao R.C., Farquhar G.D. Water use efficiency and carbon isotope discrimination in peanut under water deficit conditions. Crop Science. 1994;34:92–97. doi: 10.2135/cropsci1994.0011183X003400010016x. [DOI] [Google Scholar]
  47. Yanney-Ewusine J. Elements of tropical Ecology. London, UK: Heinemann Educational Books; 1980. [Google Scholar]
  48. Zabala N.Q. Silviculture of species, Chittagong, Bangladesh. Rome, Italy: Institute of forestry, Chittagong University (IFCU) and Food and Agricultural Organization; 1990. Silviculture of Heritiera fomes; pp. 55–57. [Google Scholar]
  49. Zimmermann M.H. Xylem structure and the ascent of sap. Berlin, Germany: Springer — Verlag; 1983. [Google Scholar]

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