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. 1976 Dec;20(3):612–620. doi: 10.1128/jvi.20.3.612-620.1976

Formation of a Sindbis virus nonstructural protein and its relation of 42S mRNA function.

M Bracha, A Leone, M J Schlesinger
PMCID: PMC355038  PMID: 994299

Abstract

Chicken embryo fibroblasts infected with an RNA- temperature-sensitive mutant (ts24) of Sindbis virus accumulated a large-molecular-weight protein (p200) when cells were shifted from the permissive to nonpermissive temperature. Appearance of p200 was accompanied by a decrease in the synthesis of viral structural proteins, but [35S]methionine tryptic peptides from p200 were different from those derived from a 140,000-molecular-weight polypeptide that contains the amino acid sequences of viral structural proteins. Among three other RNA- ts mutants that were tested for p200 formation, only one (ts21) produced this protein. The accumulation of p200 in ts24- and ts21-infected cells could be correlated with a shift in the formation of 42S and 26S viral RNA that led to an increase in the relative amounts of 42S RNA. These data indicate that p200 is translated from the nonstructural genes of the virion 42S RNA and further suggest that this RNA does not function effectively in vivo as an mRNA for the Sindbis virus structural proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson L. E., McClure W. O. An improved scintillation cocktail of high-solubilizing power. Anal Biochem. 1973 Jan;51(1):173–179. doi: 10.1016/0003-2697(73)90465-x. [DOI] [PubMed] [Google Scholar]
  2. Atkins G. J., Samuels J., Kennedy S. I. Isolation and preliminary characterization of temperature-sensitive mutants of Sindbis virus strain AR339. J Gen Virol. 1974 Dec;25(3):371–380. doi: 10.1099/0022-1317-25-3-371. [DOI] [PubMed] [Google Scholar]
  3. Burge B. W., Pfefferkorn E. R. Functional defects of temperature-sensitive mutants of Sindbis virus. J Mol Biol. 1968 Jul 14;35(1):193–205. doi: 10.1016/s0022-2836(68)80047-6. [DOI] [PubMed] [Google Scholar]
  4. Burge B. W., Pfefferkorn E. R. Isolation and characterization of conditional-lethal mutants of Sindbis virus. Virology. 1966 Oct;30(2):204–213. doi: 10.1016/0042-6822(66)90096-1. [DOI] [PubMed] [Google Scholar]
  5. Cancedda R., Schlesinger M. J. Formation of Sindbis virus capsid protein in mammalian cell-free extracts programmed with viral messenger RNA. Proc Natl Acad Sci U S A. 1974 May;71(5):1843–1847. doi: 10.1073/pnas.71.5.1843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cancedda R., Swanson R., Schlesinger M. J. Effects of different RNAs and components of the cell-free system on in vitro synthesis of Sindbis viral proteins. J Virol. 1974 Sep;14(3):652–663. doi: 10.1128/jvi.14.3.652-663.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cancedda R., Villa-Komaroff L., Lodish H. F., Schlesinger M. Initiation sites for translation of sindbis virus 42S and 26S messenger RNAs. Cell. 1975 Oct;6(2):215–222. doi: 10.1016/0092-8674(75)90012-4. [DOI] [PubMed] [Google Scholar]
  8. Clegg J. C., Kennedy S. I. Polyadenylic acid sequences in the virus RNA species of cells infected with Semliki Forest Virus. J Gen Virol. 1974 Mar;22(3):331–345. doi: 10.1099/0022-1317-22-3-331. [DOI] [PubMed] [Google Scholar]
  9. Hunter T. R., Hunt T., Knowland J., Zimmern D. Messenger RNA for the coat protein of tobacco mosaic virus. Nature. 1976 Apr 29;260(5554):759–764. doi: 10.1038/260759a0. [DOI] [PubMed] [Google Scholar]
  10. Lodish H. F. Translational control of protein synthesis. Annu Rev Biochem. 1976;45:39–72. doi: 10.1146/annurev.bi.45.070176.000351. [DOI] [PubMed] [Google Scholar]
  11. Scheele C. M., Pfefferkorn E. R. Inhibition of interjacent ribonucleic acid (26S) synthesis in cells infected by Sindbis virus. J Virol. 1969 Aug;4(2):117–122. doi: 10.1128/jvi.4.2.117-122.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Scheele C. M., Pfefferkorn E. R. Virus-specific proteins synthesized in cells infected with RNA+ temperature-sensitive mutants of Sindbis virus. J Virol. 1970 Mar;5(3):329–337. doi: 10.1128/jvi.5.3.329-337.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schlesinger M. J., Schlesinger S., Burge B. W. Identification of a second glycoprotein in Sindbis virus. Virology. 1972 Feb;47(2):539–541. doi: 10.1016/0042-6822(72)90298-x. [DOI] [PubMed] [Google Scholar]
  14. Schlesinger M. J., Schlesinger S. Large-molecular-weight precursors of sindbis virus proteins. J Virol. 1973 Jun;11(6):1013–1016. doi: 10.1128/jvi.11.6.1013-1016.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schlesinger S., Schlesinger M. J. Formation of Sindbis virus proteins: identification of a precursor for one of the envelope proteins. J Virol. 1972 Nov;10(5):925–932. doi: 10.1128/jvi.10.5.925-932.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shih D. S., Kaesberg P. Translation of brome mosaic viral ribonucleic acid in a cell-free system derived from wheat embryo. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1799–1803. doi: 10.1073/pnas.70.6.1799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Simmons D. T., Strauss J. H. Translation of Sindbis virus 26 S RNA and 49 S RNA in lysates of rabbit reticulocytes. J Mol Biol. 1974 Jun 25;86(2):397–409. doi: 10.1016/0022-2836(74)90027-8. [DOI] [PubMed] [Google Scholar]
  18. Waite M. R. Protein synthesis directed by an RNA temperature-sensitive mutant of Sindbis virus. J Virol. 1973 Feb;11(2):198–206. doi: 10.1128/jvi.11.2.198-206.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Weiss B., Schlesinger S. Defective interfering passages of Sindbis virus: chemical composition, biological activity, and mode of interference. J Virol. 1973 Oct;12(4):862–871. doi: 10.1128/jvi.12.4.862-871.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yin F. H., Lockart R. Z., Jr Maturation defects in temperature-sensitive mutants of Sindbis virus. J Virol. 1968 Jul;2(7):728–737. doi: 10.1128/jvi.2.7.728-737.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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