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. 1976 Dec;20(3):658–663. doi: 10.1128/jvi.20.3.658-663.1976

Asymmetric distribution of phosphatidylethanolamine in the membrane of vesicular stomatitis virus.

B S Fong, R C Hunt, J C Brown
PMCID: PMC355043  PMID: 186641

Abstract

The membrane-impermeable reagent trinitrobenzenesulfonate has been shown to react only with the surface components of vesicular stomatitis virus (VSV) membranes. When the amount of phosphatidylethanolamine (PE) available to modification by trinitrobenzenesulfonate in intact virions was determined, it was found that 36% of the total membrane PE was converted to the trinitrophenyl derivative. The same proportion of the total membrane PE was reactive after removal of the surface glycoprotein by trypsin digestion, but disruption of the virus membrane by sonication rendered all of the PE reactive. These results indicate that PE is asymmetrically distributed in the VSV membrane; 36% is present in the outer lipid leaflet, whereas 64% is found on the inner layer.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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