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. 1973 Jan;11(1):41–45. doi: 10.1128/jvi.11.1.41-45.1973

Ligase-Defective Bacteriophage T4 II. Physiological Studies

Robert E Koch 1
PMCID: PMC355058  PMID: 4567688

Abstract

The timing of the suppression of gene 30 (deoxyribonucleic acid ligase) mutations by rII mutations was studied by temperature shift-down experiments with a temperature-sensitive rII mutation. The rII function must remain inactivated for about 5 to 8 min at 37 C for suppression to occur, thus making suppression an early function. This result is in agreement with the timing of expression of other rII functions. A gene 30 defect can also be overcome by replacing the Na+ cation in the growth medium with the Mg2+ cation, a result similar to the relief of the lethality of rII mutations in lambda lysogens. Prior infection with bacteriophages T3 or T7, which produce their own deoxyribonucleic acid ligases, can also partially overcome the lethality of gene 30 mutations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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