Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1973 Jan;11(1):46–53. doi: 10.1128/jvi.11.1.46-53.1973

Measles Virus Ribonucleic Acid and Protein Synthesis: Effects of 6-Azauridine and Cycloheximide on Viral Replication

Allen Portner a,1, Robert H Bussell a
PMCID: PMC355059  PMID: 4630800

Abstract

Cycloheximide and 6-azauridine were employed to study the time course of measles virus protein and nucleic acid syntheses in AV3 cells. Synthesis of ribonucleic acid (RNA) essential for infectivity was first detected at 6 hr and increased concurrently with the formation of essential protein. Maximum levels of virus-specific RNA and protein were present by 18 hr, a time when only 5% of progeny virus was detected. Essential RNA and protein syntheses preceded the formation of infectious virus by at least 10 to 12 hr. The time course of RNA and protein syntheses essential for the formation of complement-fixing (CF) antigen and salt-dependent agglutinin (SDA) was also determined. RNA synthesis essential for the formation of SDA was first detected at 2 hr and was present maximally by 6 hr, whereas SDA-protein increased concurrently with the protein essential for infectivity. This suggested that the last protein essential for infectivity may be SDA. RNA synthesis essential for the formation of CF antigen was first detected at 4 hr, while CF-protein increased at 5 hr and preceded SDA-protein and protein essential for infectivity by approximately 3 hr. Reversal of inhibition of protein synthesis by cycloheximide indicated that early protein synthesis (1 to 3 hr) was required for the formation of infectious virus. The data suggest that the relatively long eclipse period observed with measles virus is related to a long maturation period rather than to late formation of early proteins, viral RNA, or structural proteins.

Full text

PDF
46

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blair C. D., Robinson W. S. Replication of Sendai virus. I. Comparison of the viral RNA and virus-specific RNA synthesis with Newcastle disease virus. Virology. 1968 Aug;35(4):537–549. doi: 10.1016/0042-6822(68)90284-5. [DOI] [PubMed] [Google Scholar]
  2. Borland R., Mahy B. W. Deoxyribonucleic acid-dependent ribonucleic acid polymerase activity in cells infected with influenza virus. J Virol. 1968 Jan;2(1):33–39. doi: 10.1128/jvi.2.1.33-39.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Choppin P. W., Holmes K. V. Replication of SV5 RNA and the effects of superinfection with poliovirus. Virology. 1967 Nov;33(3):442–451. doi: 10.1016/0042-6822(67)90119-5. [DOI] [PubMed] [Google Scholar]
  4. De Jong J. C., Winkler K. C. The multiplication of measles virus in human amnion cells in vitro. J Gen Virol. 1970 Apr;7(1):13–17. doi: 10.1099/0022-1317-7-1-13. [DOI] [PubMed] [Google Scholar]
  5. ENDERS J. F., PEEBLES T. C. Propagation in tissue cultures of cytopathogenic agents from patients with measles. Proc Soc Exp Biol Med. 1954 Jun;86(2):277–286. doi: 10.3181/00379727-86-21073. [DOI] [PubMed] [Google Scholar]
  6. ENNIS H. L., LUBIN M. CYCLOHEXIMIDE: ASPECTS OF INHIBITION OF PROTEIN SYNTHESIS IN MAMMALIAN CELLS. Science. 1964 Dec 11;146(3650):1474–1476. doi: 10.1126/science.146.3650.1474. [DOI] [PubMed] [Google Scholar]
  7. HANDSCHUMACHER R. E. Orotidylic acid decarboxylase: inhibition studies with azauridine 5'-phosphate. J Biol Chem. 1960 Oct;235:2917–2919. [PubMed] [Google Scholar]
  8. Huang A. S., Baltimore D., Bratt M. A. Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase. J Virol. 1971 Mar;7(3):389–394. doi: 10.1128/jvi.7.3.389-394.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KINGSBURY D. W. Use of actinomycin D to unmask RNA synthesis induced by Newcastle disease virus. Biochem Biophys Res Commun. 1962 Sep 25;9:156–161. doi: 10.1016/0006-291x(62)90106-7. [DOI] [PubMed] [Google Scholar]
  10. Kingsbury D. W. Newcastle disease virus RNA. II. Preferential synthesis of RNA complementary to parental viral RNA by chick embryo cells. J Mol Biol. 1966 Jun;18(1):204–214. doi: 10.1016/s0022-2836(66)80086-4. [DOI] [PubMed] [Google Scholar]
  11. Kingsbury D. W. Replication and functions of myxovirus ribonucleic acids. Prog Med Virol. 1970;12:49–77. [PubMed] [Google Scholar]
  12. Mahy B. W., Hutchinson J. E., Barry R. D. Ribonucleic acid polymerase induced in cells infected with Sendai virus. J Virol. 1970 Jun;5(6):663–671. doi: 10.1128/jvi.5.6.663-671.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Matumoto M., Arita M., Oda M. Enhancement of measles virus replication by actinomycin D. Jpn J Exp Med. 1965 Oct;35(5):319–329. [PubMed] [Google Scholar]
  14. Matumoto M. Multiplication of measles virus in cell cultures. Bacteriol Rev. 1966 Mar;30(1):152–176. doi: 10.1128/br.30.1.152-176.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Numazaki Y., Karzon D. T. Density separable fractions during growth of measles virus. J Immunol. 1966 Oct;97(4):458–469. [PubMed] [Google Scholar]
  16. Numazaki Y., Karzon D. T. Soluble antigen of measles virus. J Immunol. 1966 Oct;97(4):470–476. [PubMed] [Google Scholar]
  17. Robinson W. S. Ribonucleic acid polymerase activity in Sendai virions and nucleocapsid. J Virol. 1971 Jul;8(1):81–86. doi: 10.1128/jvi.8.1.81-86.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Robinson W. S. Sendai virus RNA synthesis and nucleocapsid formation in the presence of cycloheximide. Virology. 1971 Jun;44(3):494–502. doi: 10.1016/0042-6822(71)90362-x. [DOI] [PubMed] [Google Scholar]
  19. SCHOLTISSEK C., ROTT R., HAUSEN P., HAUSEN H., SCHAEFER W. Conparative studies of RNA and protein synthesis with a myxovirus and a small polyhedral virus. Cold Spring Harb Symp Quant Biol. 1962;27:245–257. doi: 10.1101/sqb.1962.027.001.024. [DOI] [PubMed] [Google Scholar]
  20. Schluederberg A., Nakamura M. A salt-dependent hemagglutinating particle from measles-infected cells. Virology. 1967 Oct;33(2):297–306. doi: 10.1016/0042-6822(67)90148-1. [DOI] [PubMed] [Google Scholar]
  21. Scholtissek C., Rott R. Metabolic changes in chick fibroblasts after infection with Newcastle disease virus. Nature. 1965 May 15;206(985):729–730. doi: 10.1038/206729a0. [DOI] [PubMed] [Google Scholar]
  22. WATERSON A. P. MEASLES VIRUS. Arch Gesamte Virusforsch. 1965;16:57–80. doi: 10.1007/BF01253793. [DOI] [PubMed] [Google Scholar]
  23. WHITE D. O., DAY H. M., BATCHELDER E. J., CHEYNE I. M., WANSBROUGH A. J. DELAY IN THE MULTIPLICATION OF INFLUENZA VIRUS. Virology. 1965 Feb;25:289–302. doi: 10.1016/0042-6822(65)90207-2. [DOI] [PubMed] [Google Scholar]
  24. WILSON D. E., LOGERFO P. INHIBITION OF RIBONUCLEIC ACID SYNTHESIS IN NEWCASTLE DISEASE VIRUS-INFECTED CELLS BY PUROMYCIN AND 6-AZAURIDINE. J Bacteriol. 1964 Dec;88:1550–1555. doi: 10.1128/jb.88.6.1550-1555.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES