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. 2010 Sep 5;16(2):207–213. doi: 10.1007/s12298-010-0023-9

Assessment of genetic fidelity of micropropagated date palm (Phoenix dactylifera L.) plants by RAPD and ISSR markers assay

Nitish Kumar 1,✉, Arpan R Modi 1, Amritpal S Singh 1, Bhavesh B Gajera 1, Armi R Patel 1, Mukesh P Patel 1, Naraynan Subhash 1
PMCID: PMC3550608  PMID: 23572971

Abstract

RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeats) markers assay were employed to validate the genetic stability of date palm (Phoenix dactylifera L.) plants multiplied through somatic embryogenesis with upto forty two in vitro subcultures. Out of the 160 RAPD and 21 ISSR primers screened, 30 RAPD and 12 ISSR primers produced a total of 347 (246 RAPDs + 101 ISSRs) clear, distinct and reproducible amplicons, which were monomorphic across all micropropagated plants (27) studied. Thus, a total 8592 bands (number of plants analysed x number of amplicons with all the primers) were generated which exhibited homogeneous banding patterns with both RAPD and ISSR markers. These results indicate that the micropropagation protocol developed by us for rapid in vitro multiplication is appropriate and suitable for clonal propagation of date palm and corroborated the fact that somatic embryogenesis can also be used as one of the safest modes for production of true-to-type plants.

Keywords: Date palm, genetic stability, ISSR, molecular marker, RAPD

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