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. 1973 Jan;11(1):87–97. doi: 10.1128/jvi.11.1.87-97.1973

Gene Expression During the Development of Bacillus subtilis Bacteriophage φ29 II. Resolution of Viral-Specific Ribonucleic Acid Molecules

D J Loskutoff 1, J J Pène 1
PMCID: PMC355064  PMID: 4630803

Abstract

The ribonucleic acid (RNA) specified by bacteriophage φ29 was isolated under conditions which minimized physical and enzymatic degradation, reduced aggregation, and enriched for completed molecules. This RNA was fractionated both by sedimentation through sucrose density gradients and electrophoresis through polyacrylamide gels to measure the size and relative amount of each component. Early RNA consisted of six components of molecular weight 0.75 × 106, 0.44 × 106, 0.37 × 106, 0.25 × 106, 0.09 × 106, and 0.04 × 106, accounting for 35% of the coding capacity of φ29 deoxyribonucleic acid (DNA). All of these components except the one at 0.44 × 106 were detected when infection occurred in the presence of chloramphenicol. Synthesis of the major early component (0.75 × 106) ceased shortly after the onset of viral DNA synthesis. The other species of early RNA were synthesized throughout the latent period. Three additional components, 1.75 × 106, 0.93 × 106, and 0.07 × 106, appear at late times. The two large RNAs may be polycistronic messenger RNAs corresponding to the seven viral capsid proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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