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. 2009 Feb 26;14(4):337–346. doi: 10.1007/s12298-008-0032-0

In vitro clonal propagation of bael (Aegle marmelos Corr.) CV. CISH-B1 through enhanced axillary branching

Rajesh Pati 1, Ramesh Chandra 1, Ugam Kumari Chauhan 2,✉, Maneesh Mishra 1, Navin Srivastava 1
PMCID: PMC3550643  PMID: 23572900

Abstract

Rapid clonal micropropagation protocol of Aegle marmelos (L.) Corr. cv. CISH-B1 was achieved by nodal stem segment of mature bearing tree. Three centimeter long shoots having one axillary bud excised from 10–15th nodal region of shoots during September gave quick in vitro bud burst (5.33 days) when cultured on MS medium supplemented with BAP, 8.84 μM + IAA 5.7 μM. The maximum number of proliferated shoots (9.0/explant) were obtained on same medium supplemented with BAP 8.84 μM + IAA 5.7 μM. The micro shoots were rooted (100 %) on + IAA 5.7 μM. In vitro rooted plants were acclimatized on autoclaved coconut husk containing plant salt mixture and under shade net house (50 % shade 70–80 % RH). The plants were established in the field after acclimatization. The micropropagated plants were tested for its genetic fidelity using 13 RAPD, 3 ISSR and 2 DAMD primers. Profile obtained by all the three Single Primer Amplification Reaction (SPAR) technique from mother tree and micropropagated plants revealed genetic integrity of micropropagated plants with that of mother tree.

Key words: Micropropagation, Aegle marmelos, PCR, ISSR, RAPD, DAMD, SPAR

Full Text

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Abbreviations

IAA

Indole-3-acetic acid

IBA

Indole-3-butyric acid

BAP

Benzyl amino purine

ADS

Adenine sulphate

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