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. 2010 Dec 9;16(4):375–378. doi: 10.1007/s12298-010-0037-3

Development of EST-based new SSR markers in seabuckthorn

Ankit Jain 1, Rajesh Ghangal 1, Atul Grover 1,3, Saurabh Raghuvanshi 2, Prakash C Sharma 1,✉
PMCID: PMC3550651  PMID: 23572988

Abstract

EST-based SSR markers were developed by screening a collection of 1584 clustered ESTs of seabuckthorn (Hippophae rhamnoides). PCR primers were designed for the amplification of 30 microsatellite loci. Two to five allelic bands were displayed by nine primer pairs in H. rhamnoides genotypes and by eleven primer pairs in H. salicifolia genotypes. None of the thirty primer pairs detected polymorphism in H. tibetana genotypes. Considering the high polymorphism detected in the tested genotypes and their direct origin from the genic regions, these EST-SSR markers hold immense promise in seabuckthorn genome analysis, molecular breeding and population genetics.

Keywords: Hippophae rhamnoides, Expressed sequence tags, Simple sequence repeats (SSRs), Cross-amplification

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Acknowledgements

This research and AJ was supported by Defense Research and Development Organization (DRDO), India through a sponsored project (Project No. LSRB-146/FS/2008). RG thanks Indian Council of Medical Research (ICMR), India for financial support. Authors wish to thank Defense Institute of High Altitude Research (DIHAR), Leh, India for providing genotypes used in this study.

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