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. 2011 Jan 6;16(4):379–382. doi: 10.1007/s12298-010-0043-5

Alginate-encapsulation of shoot tips of jojoba [Simmondsia chinensis (Link) Schneider] for germplasm exchange and distribution

Sunil Kumar 1,2,✉, Manoj K Rai 1, Narender Singh 2, Manisha Mangal 1
PMCID: PMC3550652  PMID: 23572989

Abstract

Shoot tips excised from in vitro proliferated shoots derived from nodal explants of jojoba [Simmondsia chinensis (Link) Schneider] were encapsulated in calcium alginate beads for germplasm exchange and distribution. A gelling matrix of 3 % sodium alginate and 100 mM calcium chloride was found most suitable for formation of ideal calcium alginate beads. Best response for shoot sprouting from encapsulated shoot tips was recorded on 0.8 % agar-solidified full-strength MS medium. Rooting was induced upon transfer of sprouted shoots to 0.8 % agar-solidified MS medium containing 1 mg l−1 IBA. About 70 % of encapsulated shoot tips were rooted and converted into plantlets. Plants regenerated from encapsulated shoot tips were acclimatized successfully. The present encapsulation approach could also be applied as an alternative method of propagation of desirable elite genotype of jojoba.

Keywords: Acclimatization, Conservation, Plant regeneration, Simmondsia chinensis, Synthetic seeds

Full Text

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Acknowledgements

The author (S.K.) is thankful to Dr. A.K Dhawan Director (T), Centre for Plant Biotechnology, CCS Haryana Agricultural University Campus, Hisar for the providing lab facility and scientific, technical and project staff for their cooperation and help during the course of investigation.

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