Abstract
Some biological characteristics and bioassay of a polyhedral virus isolated from normal-appearing strain HB-301 of Entamoeba histolytica are described. The virus produced lysis of susceptible strains of E. histolytica, yet it caused no cytopathological effect in the host strain, HB-301. The virus and host amoeba existed in an equilibrium; approximately 104 mean infective dose units of virus were produced per million HB-301 amoebae. Superinfection and antiviral antiserum treatment failed to disturb this equilibrium permanently. The mechanism of viral persistence in strain HB-301 amoebae remains to be determined. Purification of the virus was attempted. Ninety-nine percent of the viral infectivity was associated with a low-speed pellet which consisted of complexes of virus and cellular membranes. Various treatments of this low-speed pellet failed to release virus. Biologically active, membrane-free virus of low titer was prepared by differential centrifugation of supernatant solutions and employed in electron microscopy and other studies.
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- ANDERSON T. F. The morphology and osmotic properties of bacteriophage systems. Cold Spring Harb Symp Quant Biol. 1953;18:197–203. doi: 10.1101/sqb.1953.018.01.030. [DOI] [PubMed] [Google Scholar]
- BLACK F. L., MELNICK J. L. Microepidemiology of poliomyelitis and herpes-B infections: spread of the viruses within tissue cultures. J Immunol. 1955 Mar;74(3):236–242. [PubMed] [Google Scholar]
- BLACK P. H., CRAWFORD E. M., CRAWFORD L. V. THE PURIFICATION OF SIMIAN VIRUS 40. Virology. 1964 Nov;24:381–387. doi: 10.1016/0042-6822(64)90175-8. [DOI] [PubMed] [Google Scholar]
- Diamond L. S. Improved method for the monoxenic cultivation of Entamoeba histolytica Schaudinn, 1903 and E. histolytica-like amebae with trypanosomatids. J Parasitol. 1968 Aug;54(4):715–719. [PubMed] [Google Scholar]
- Diamond L. S., Mattern C. F., Bartgis I. L. Viruses of Entamoeba histolytica. I. Identification of transmissible virus-like agents. J Virol. 1972 Feb;9(2):326–341. doi: 10.1128/jvi.9.2.326-341.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Diamond L. S. Techniques of axenic cultivation of Entamoeba histolytica Schaudinn, 1903 and E. histolytica-like amebae. J Parasitol. 1968 Oct;54(5):1047–1056. [PubMed] [Google Scholar]
- LARK K. G., ADAMS M. H. The stability of phages as a function of the ionic environment. Cold Spring Harb Symp Quant Biol. 1953;18:171–183. doi: 10.1101/sqb.1953.018.01.028. [DOI] [PubMed] [Google Scholar]
- Mattern C. F., Diamond L. S., Daniel W. A. Viruses of Entamoeba histolytica. II. Morphogenesis of the polyhedral particle (ABRM 2 leads to HK-9) leads to HB-301 and the filamentous agent (ABRM) 2 leads to HK-9. J Virol. 1972 Feb;9(2):342–358. doi: 10.1128/jvi.9.2.342-358.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mattern C. F., Takemoto K. K., DeLeva A. M. Electron microscopic observations on multiple polyoma virus-related particles. Virology. 1967 Jul;32(3):378–392. doi: 10.1016/0042-6822(67)90288-7. [DOI] [PubMed] [Google Scholar]
- NISHMI M., KELLER R. Observations on the mode of spread of vaccinia virus in HeLa cell monolayer cultures. Virology. 1962 Jan;16:91–92. doi: 10.1016/0042-6822(62)90206-4. [DOI] [PubMed] [Google Scholar]
- STOKER M. G. Mode of intercellular transfer of herpes virus. Nature. 1958 Nov 29;182(4648):1525–1526. doi: 10.1038/1821525a0. [DOI] [PubMed] [Google Scholar]
- WALKER D. L. THE VIRAL CARRIER STATE IN ANIMAL CELL CULTURES. Prog Med Virol. 1964;6:111–148. [PubMed] [Google Scholar]