Abstract
The addition of purified, noninfectious, double-stranded RNA of bovine enterovirus, a picornavirus, to intact cells in culture results in a rapid cessation of cellular polypeptide synthesis. This inhibition is specific for host cell protein synthesis since the translation of picornavirus-specific proteins is not affected by the double-stranded viral RNA.
Full text
PDF





Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ascione R., Vande Woude G. F. Ribosomal factors effecting the stimulation of cell-free protein synthesis in the prescence of foot-and-mouth disease virus ribonucleic acid. Biochem Biophys Res Commun. 1971 Oct 1;45(1):14–21. doi: 10.1016/0006-291x(71)90043-x. [DOI] [PubMed] [Google Scholar]
- Bishop J. M., Koch G. Purification and characterization of poliovirus-induced infectious double-stranded ribonucleic acid. J Biol Chem. 1967 Apr 25;242(8):1736–1743. [PubMed] [Google Scholar]
- Bishop J. M., Summers D. F., Levintow L. Characterization of ribonuclease-resistant RNA from poliovirus-infected HeLa cells. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1273–1281. doi: 10.1073/pnas.54.4.1273. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chao J., Chao L., Speyer J. The inhibition of f2 RNA directed E. coli protein synthesis by poly rI-rC in vitro. Biochem Biophys Res Commun. 1971 Nov;45(4):1096–1102. doi: 10.1016/0006-291x(71)90450-5. [DOI] [PubMed] [Google Scholar]
- Cordell-Stewart B., Taylor M. W. Effect of double-stranded viral RNA on mammalian cells in culture. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1326–1330. doi: 10.1073/pnas.68.6.1326. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Darnbrough C., Hunt T., Jackson R. J. A complex between met-tRNA F and native 40S subunits in reticulocyte lysates and its disappearance during incubation with double-stranded RNA. Biochem Biophys Res Commun. 1972 Sep 26;48(6):1556–1564. doi: 10.1016/0006-291x(72)90891-1. [DOI] [PubMed] [Google Scholar]
- Ehrenfeld E., Hunt T. Double-stranded poliovirus RNA inhibits initiation of protein synthesis by reticulocyte lysates. Proc Natl Acad Sci U S A. 1971 May;68(5):1075–1078. doi: 10.1073/pnas.68.5.1075. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hunt T., Ehrenfeld E. Cytoplasm from poliovirus-infected HeLa cells inhibits cell-free haemoglobin synthesis. Nat New Biol. 1971 Mar 17;230(11):91–94. doi: 10.1038/newbio230091a0. [DOI] [PubMed] [Google Scholar]
- Koschel K., Täuber H., Wecker E. Early functions of poliovirus. I. The inhibition of cellular protein synthesis in HeLa cells after infection with active and inactivated poliovirus. Z Naturforsch B. 1971 Aug;26(8):798–803. doi: 10.1515/znb-1971-0813. [DOI] [PubMed] [Google Scholar]
- Penman S. RNA metabolism in the HeLa cell nucleus. J Mol Biol. 1966 May;17(1):117–130. doi: 10.1016/s0022-2836(66)80098-0. [DOI] [PubMed] [Google Scholar]
- Penman S., Summers D. Effects on host cell metabolism following synchronous infection with poliovirus. Virology. 1965 Dec;27(4):614–620. doi: 10.1016/0042-6822(65)90187-x. [DOI] [PubMed] [Google Scholar]
- Stern R., Friedman R. M. Ribonucleic acid synthesis in animal cells in the presence of actinomycin. Biochemistry. 1971 Sep 28;10(20):3635–3645. doi: 10.1021/bi00796a001. [DOI] [PubMed] [Google Scholar]
- Taylor M. W., Cordell B., Souhrada M., Prather S. Viruses as an aid to cancer therapy: regression of solid and ascites tumors in rodents after treatment with bovine enterovirus. Proc Natl Acad Sci U S A. 1971 Apr;68(4):836–840. doi: 10.1073/pnas.68.4.836. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wall R., Taylor M. W. Mengovirus RNA synthesis in productive and restrictive cell lines. Virology. 1970 Sep;42(1):78–86. doi: 10.1016/0042-6822(70)90240-0. [DOI] [PubMed] [Google Scholar]
- Wiegers K. J., Koch G. Interaction of poliovirus-induced double-stranded RNA with HeLa cells. Arch Biochem Biophys. 1972 Jan;148(1):89–96. doi: 10.1016/0003-9861(72)90119-1. [DOI] [PubMed] [Google Scholar]