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Journal of Virology logoLink to Journal of Virology
. 1973 Feb;11(2):279–286. doi: 10.1128/jvi.11.2.279-286.1973

Isolation of an Infectious Ribonucleoprotein from Vesicular Stomatitis Virus Containing an Active RNA Transcriptase

J F Szilágyi 1, L Uryvayev 1,1
PMCID: PMC355093  PMID: 4347429

Abstract

A ribonucleoprotein complex (TNP) containing an active RNA polymerase was isolated from purified vesicular stomatitis virus particles. The TNP sedimented through a sucrose gradient as a single band and appeared under the electron microscope as discrete long filaments in a spiral configuration. TNP contained one major and two minor polypeptides, but not the polypeptides associated with the outer coat of vesicular stomatitis virus. BHK-21 clone 13 cells could be infected with TNP, yielding infectious virus particles.

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Selected References

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  1. Baltimore D. Expression of animal virus genomes. Bacteriol Rev. 1971 Sep;35(3):235–241. doi: 10.1128/br.35.3.235-241.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baltimore D., Huang A. S., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572–576. doi: 10.1073/pnas.66.2.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baltimore D., Huang A., Manly K. F., Rekosh D., Stampfer M. The synthesis of protein by mammalian RNA viruses. In: strategy of the viral genome. Ciba Found Symp. 1971:101–110. doi: 10.1002/9780470719824.ch6. [DOI] [PubMed] [Google Scholar]
  4. Bishop D. H., Roy P. Dissociation of vesicular stomatitis virus and relation of the virion proteins to the viral transcriptase. J Virol. 1972 Aug;10(2):234–243. doi: 10.1128/jvi.10.2.234-243.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bishop D. H., Roy P. Kinetics of RNA synthesis by vesicular stomatitis virus particles. J Mol Biol. 1971 May 14;57(3):513–527. doi: 10.1016/0022-2836(71)90106-9. [DOI] [PubMed] [Google Scholar]
  6. Brown F., Cartwright B., Crick J., Smale C. J. Infective virus substructure from vesicular stomatitis virus. J Virol. 1967 Apr;1(2):368–373. doi: 10.1128/jvi.1.2.368-373.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Emerson S. U., Wagner R. R. Dissociation and reconstitution of the transcriptase and template activities of vesicular stomatitis B and T virions. J Virol. 1972 Aug;10(2):297–309. doi: 10.1128/jvi.10.2.297-309.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Huang A. S., Baltimore D., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus. 3. Multiple complementary messenger RNA molecules. Virology. 1970 Dec;42(4):946–957. doi: 10.1016/0042-6822(70)90343-0. [DOI] [PubMed] [Google Scholar]
  9. Huang A. S., Wagner R. R. Comparative sedimentation coefficients of RNA extracted from plaque-forming and defective particles of vesicular stomatitis virus. J Mol Biol. 1966 Dec 28;22(2):381–384. doi: 10.1016/0022-2836(66)90143-4. [DOI] [PubMed] [Google Scholar]
  10. Kang C. Y., Prevec L. Proteins of vesicular stomatitis virus. I. Polyacrylamide gel analysis of viral antigens. J Virol. 1969 Apr;3(4):404–413. doi: 10.1128/jvi.3.4.404-413.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Katz E., Moss B. Vaccinia virus structural polypeptide derived from a high-molecular-weight precursor: formation and integration into virus particles. J Virol. 1970 Dec;6(6):717–726. doi: 10.1128/jvi.6.6.717-726.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. MACPHERSON I., STOKER M. Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence. Virology. 1962 Feb;16:147–151. doi: 10.1016/0042-6822(62)90290-8. [DOI] [PubMed] [Google Scholar]
  14. Mudd J. A., Summers D. F. Polysomal ribonucleic acid of vesicular stomatitis virus-infected HeLa cells. Virology. 1970 Dec;42(4):958–968. doi: 10.1016/0042-6822(70)90344-2. [DOI] [PubMed] [Google Scholar]
  15. Mudd J. A., Summers D. F. Protein synthesis in vesicular stomatitis virus-infected HeLa cells. Virology. 1970 Oct;42(2):328–340. doi: 10.1016/0042-6822(70)90277-1. [DOI] [PubMed] [Google Scholar]
  16. Nakai T., Howatson A. F. The fine structure of vesicular stomatitis virus. Virology. 1968 Jun;35(2):268–281. doi: 10.1016/0042-6822(68)90267-5. [DOI] [PubMed] [Google Scholar]
  17. Wagner R. R., Schnaitman T. A., Snyder R. M. Structural proteins of vesicular stomatitis viruses. J Virol. 1969 Apr;3(4):395–403. doi: 10.1128/jvi.3.4.395-403.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wunner W. H., Pringle C. R. Protein synthesis in BHK21 cells infected with vesicular stomatitis virus. I. ts Mutants of the Indiana serotype. Virology. 1972 Apr;48(1):104–111. doi: 10.1016/0042-6822(72)90118-3. [DOI] [PubMed] [Google Scholar]

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