Abstract
A method has been developed for the concentration of herpesviruses by negative pressure ultrafiltration through dialysis tubing. The procedure results in high titer concentrates of unaggregated, morphologically intact viral particles with a virtually quantitative recovery of infectivity.
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Selected References
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- Abodeely R. A., Lawson L. A., Randall C. C. Morphology and entry of enveloped and deenveloped equine abortion (herpes) virus. J Virol. 1970 Apr;5(4):513–523. doi: 10.1128/jvi.5.4.513-523.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chambers R. W., Rose J. A., Rabson A. S., Bond H. E., Hall W. T. Propagation and purification of high-titer human cytomegalovirus. Appl Microbiol. 1971 Nov;22(5):914–918. doi: 10.1128/am.22.5.914-918.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cline G. B., Nunley C. E., Anderson N. G. Improved continuous flow centrifugation with banding. Nature. 1966 Oct 29;212(5061):487–489. doi: 10.1038/212487a0. [DOI] [PubMed] [Google Scholar]
- Dales S., Silverberg H. Viropexis of herpes simplex virus by HeLa cells. Virology. 1969 Mar;37(3):475–480. doi: 10.1016/0042-6822(69)90232-3. [DOI] [PubMed] [Google Scholar]
- McCombs R. M. Concentration and purification of herpesviruses (simplex, cytomegalo, and EB) in a zonal ultracentrifuge. Appl Microbiol. 1969 Apr;17(4):636–638. doi: 10.1128/am.17.4.636-638.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roizman B., Spear P. G. Preparation of herpes simplex virus of high titer. J Virol. 1968 Jan;2(1):83–84. doi: 10.1128/jvi.2.1.83-84.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vonka V., Benyesh-Melnick M. Interactions of human cytomegalovirus with human fibroblasts. J Bacteriol. 1966 Jan;91(1):213–220. doi: 10.1128/jb.91.1.213-220.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vonka V., Benyeshmelnick M. Thermoinactivation of human cytomegalovirus. J Bacteriol. 1966 Jan;91(1):221–226. doi: 10.1128/jb.91.1.221-226.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wentworth B. B., French L. Plaque assay of Herpesvirus hominis on human embryonic fibroblasts. Proc Soc Exp Biol Med. 1969 Jun;131(2):588–592. doi: 10.3181/00379727-131-33932. [DOI] [PubMed] [Google Scholar]
- Wentworth B. B., French L. Plaque assay of cytomegalovirus strains of human origin. Proc Soc Exp Biol Med. 1970 Nov;135(2):253–258. doi: 10.3181/00379727-135-35031. [DOI] [PubMed] [Google Scholar]