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. 2010 Sep 8;47(4):420–425. doi: 10.1007/s13197-010-0069-2

Effect of pasteurization of shell egg on its quality characteristics under ambient storage

E Shenga 1, R P Singh 1,✉, A S Yadav 1
PMCID: PMC3551002  PMID: 23572663

Abstract

Three thermal processes viz. dry (55°C, 2 h), moist (57°C, 5 min) and microwave (power 9, 20 sec) were studied to determine their efficacy for the pasteurization of intact chicken eggs based on the extent of inactivation of artificially inoculated Salmonella typhimurium (ST) in the yolk of shell eggs and alteration in albumen protein solubility (APS). Moist heat treatment was superior to others as it brought about 2 log cfu/ml reduction of inoculated ST in much less time than dry heating but changes in APS were not significant. Subsequent quality evaluation of normal (uninoculated) eggs subjected to moist heat pasteurization during 15 days of ambient (35°C, 36% RH) (35 ± 0.5°C, 36 ± 2% RH)storage revealed no significant effect on percent loss in egg weight, albumen pH, viscosity of albumen and yolk and thiobarbituric acid values between pasteurized and unpasteurized eggs. Pasteurization had no adverse effect on foam volume and foam stability of albumen during storage in comparison to those of raw eggs. Naturally occurring aerobic mesophilic bacteria, coliforms, staphylococci, yeast and moulds on the egg shell surface and in egg contents got markedly reduced by pasteurization of shell eggs and their multiplication also retarded during storage. Both pasteurized and raw eggs remained fairly acceptable sensorily up to 10 days of storage at ambient conditions.

Keywords: Shell egg, Pasteurization, Salmonella typhimurium, Inactivation

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References

  1. Anand S.K., Pandey N.K., Mahapatra C.M., Verma S.S. Spoilage and pathogenic microflora of fresh chicken eggs. Indian J Poult Sci. 1993;28:125–129. [Google Scholar]
  2. Official methods of analysis. 16th edn. Washington DC: Association of Official Analytical Chemists; 1995. [Google Scholar]
  3. Compendium of methods for the microbiological examination of foods. 4th edn. Washington DC: American Public Health Association; 2001. [Google Scholar]
  4. Bajaj B.K., Sharma V., Thakur R.L. Prevalence and antibiotic resistance profiles of Salmonella spp in poultry eggs. J Food Sci Technol. 2003;40:682–684. [Google Scholar]
  5. Baldwin R.E. Functional properties in foods. In: Stadelman W. J., Cotterill O. J., editors. Egg Science and Technology. 2nd edn. Connecticut: AVI Publ Co; 1977. pp. 246–277. [Google Scholar]
  6. Cotterill O.J., Marion W.W., Naber E.C. A nutrient re-evaluation of shell eggs. Poult Sci. 1977;56:1927–1934. doi: 10.3382/ps.0561927. [DOI] [PubMed] [Google Scholar]
  7. Cunningham F.E. Egg product pasteurization. In: Stadelman W. J., Cotterill O. J., editors. Egg science and technology. 2nd edn. Connecticut: AVI Publ Co; 1977. pp. 161–186. [Google Scholar]
  8. Funk E.M. The relation of yolk index determined in natural position to the yolk index as determined after separating the yolk from albumen. Poult Sci. 1948;27:376–380. [Google Scholar]
  9. Hank C.R., Kunkel M.E., Dawson P.L., Action J.C., Wardlaw F. B., Jr The effect of shell egg pasteurization on the protein quality of albumen. Poult Sci. 2001;80:821–824. doi: 10.1093/ps/80.6.821. [DOI] [PubMed] [Google Scholar]
  10. Haugh R.R. The Haugh unit for measuring egg quality. US Egg and Poult Mag. 1937;43:552–555. [Google Scholar]
  11. Henzler D.J., Kradel D.C., Sischo W.M. Management and environmental risk factors for Salmonella enteritidis contamination of eggs. Am J Vet Res. 1998;59:824–829. [PubMed] [Google Scholar]
  12. Heiman V., Carver J.S. The albumen index as a physical measurement of observed egg quality. Poult Sci. 1936;15:141–148. [Google Scholar]
  13. Hou H., Singh R.K., Muriana P.M., Stadelman W.J. Pasteurization of intact shell eggs. Food Microbiol. 1996;13:93–101. doi: 10.1006/fmic.1996.0012. [DOI] [Google Scholar]
  14. Humphrey T.J. Contamination of egg shell and contents with Salmonella enteritidis: A review. Int J Food Microbiol. 1994;21:31–40. doi: 10.1016/0168-1605(94)90197-X. [DOI] [PubMed] [Google Scholar]
  15. Humphrey T.J., Baskerville A., Mawer S., Rowe B., Hopper S. Salmonella enteritidis from the contents of intact PT4 eggs: A study involving naturally infected hens. Epidemiol Infect. 1989;103:415–423. doi: 10.1017/S0950268800030818. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Humphrey T.J., Whitehead A., Gawler A.H., Henley A., Rowe B. Numbers of Salmonella enteritidis in the contents of naturally contaminated hens’ eggs. Epidemiol Infect. 1991;106:489–496. doi: 10.1017/S0950268800067546. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Jeantet R., Baron F., Nau F., Roignant M., Brule G. High intensity pulsed electrical fields applied to egg white: Effect on Salmonella enteritidis inactivation and protein denaturation. J Food Prot. 1999;62:1381–1386. doi: 10.4315/0362-028x-62.12.1381. [DOI] [PubMed] [Google Scholar]
  18. Krishnamoorthy P., Paul W.M., Premkumar E.S., Govindrajan D. Evaluation of pathogenic coliform bacteria in fresh marketable table eggs in and around Chennai city. Indian J Anim Health. 2003;42:120–123. [Google Scholar]
  19. Kumar S., Panda B., Reddy M.S., Rao R.J. Studies on comparative efficacy of oil coating and lime sealing on the preservation of shell eggs at room temperature. J Food Sci Technol. 1969;6:9–11. [Google Scholar]
  20. Messens W., Grijspeerdt K., De Reu K., De Ketelaere B., Mertens K., Bamelis F., Kemp B., De Baerdemaeker J., Decuypere E., Herman L. Egg shell penetration of various types of hens eggs by Salmonella enterica serovar enteritidis. J Food Protec. 2007;70:623–628. doi: 10.4315/0362-028x-70.3.623. [DOI] [PubMed] [Google Scholar]
  21. Modi V.K., Sheela P.N., Mahendrakar N.S. Egg albumen cubes and egg yolk cubes and their quality changes during storage. J Food Sci Technol. 2008;45:161–165. [Google Scholar]
  22. Morr C.V., German J.E., Kinsella J.M., Regenstein J.P., Buren V., Kilara J.A., Lewis B.A., Mangino M.E. A collaborative study to develop a standardized food protein solubility procedure. J. Food Sci. 1985;50:1715–1717. doi: 10.1111/j.1365-2621.1985.tb10572.x. [DOI] [Google Scholar]
  23. Panda P.C., Panda B. Bacteriological condition of shell eggs marketed in India. Indian J Poult Sci. 1973;8:11–18. [Google Scholar]
  24. Pike O.A., Peng I.C. Stability of shell egg and liquid yolk to lipid oxidation. Poult Sci. 1985;64:1470–1475. [Google Scholar]
  25. Reu K.D., Messens W., Heyndricick M., Rodenburg T.B., Uyttendaele M., Herman L. Bacterial contamination of table eggs and the influence of housing systems. World Poult Sci J. 2008;64:5–19. [Google Scholar]
  26. Snedecor G.W., Cochran W.G. Statistical methods. 8th edn. Ames, Iowa: Iowa State University press; 1994. [Google Scholar]
  27. Stadelman W.J., Rhorer A.R. Quality improvement of hard cooked eggs. Poult Sci. 1984;63:949–953. [Google Scholar]
  28. Stadelman W.J., Singh R.K., Muriana P.M., Hou H. Pasteurization of eggs in shell. Poult Sci. 1996;75:1122–1125. doi: 10.3382/ps.0751122. [DOI] [PubMed] [Google Scholar]
  29. Suresh T., Hatha A.A., Sreenivasan D., Sangeetha N., Lashmanaperumalsamy P. Prevalence and antimicrobial resistance of Salmonella enteritidis and other salmonellae in the eggs and egg-storing trays from retail markets of Coimbatore, South India. Food Microbiol. 2006;23:294–299. doi: 10.1016/j.fm.2005.04.001. [DOI] [PubMed] [Google Scholar]
  30. Tarladgis B.G., Watts B.M., Younathan M.T., Durgan L., Jr A distillation method for quantitative determination of malonaldehyde in foods. J Am Oil Chem Soc. 1960;37:44–48. doi: 10.1007/BF02630824. [DOI] [Google Scholar]
  31. Van Lith L.A.J.T., Putirulan F.F., Mulder R.W.A.W. Pasteurization of table eggs to eliminate Salmonella. Archiv Gefluegelkund. 1995;59:157–160. [Google Scholar]
  32. Yadav A.S., Singh R.P., Kirupasankar M. Occurrence of Salmonella in chicken eggs collected from selected poultry farms and marketing channels. Indian J Anim Sci. 2008;78:887–890. [Google Scholar]

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