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. 2010 Sep 8;47(4):458–460. doi: 10.1007/s13197-010-0077-2

Citric acid production by Aspergillus niger van. Tieghem MTCC 281 using waste apple pomace as a substrate

Dinesh Kumar 1,2,✉, Rachna Verma 1,2, T C Bhalla 1,2
PMCID: PMC3551004  PMID: 23572671

Abstract

A solid state fermentation process was tried for the production of citric acid from apple pomace left after juice extraction using Aspergillus niger van. Tieghem MTCC 281 spores as inoculum (36.8 × 104 spores/100 g of pomace). The yield of citric acid was optimized by varying the amount of methanol (1–5% v/w), temperature (25–35°C) and time of incubation (1–7 days) for fermentation process. Optimum yield of citric acid (4.6 g/100 g of pomace) was recorded with 4% (v/w) methanol after 5 days of incubation at 30°C.

Keywords: Citric acid production, Waste apple pomace, Aspergillus niger van. Tieghem MTCC 281

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