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. 2010 Sep 8;47(4):432–436. doi: 10.1007/s13197-010-0071-8

Effect of sodium lactate as cryostabilizer on physico-chemical attributes of croaker (Johnius gangeticus) muscle protein

S S Dey 1, K C Dora 1,✉
PMCID: PMC3551008  PMID: 23572665

Abstract

Effect of sodium lactate as cryostabilizer on physico-chemical attributes of croaker (Johnius gangeticus) fish muscle protein was studied during freezing and frozen (−20 ± 2°C) storage for 3 months. Minced meat was mixed with 4% sucrose, 4% sorbitol, and 0.3% sodium tri poly phosphate (STPP) (T1), minced meat was mixed with 6% (w/v) sodium lactate and 0.3% STPP (T2) and control (C) was without any additive. The decreasing rate of Ca2+ ATPase activity, thaw drip, water holding capacity and relative viscosity in T1 and T2 samples from that of C was significantly lower, indicating higher protective effect of additives. In case of cryoprotectant treated samples, the degradation of myosin heavy chain was much lower than that of C which prevents the aggregation and subsequent insolubilization of myosin during frozen storage. The sodium lactate prevented Ca2+ATPase activity more than that of sucrose/sorbitol during isothermal storage at −20 ± 2°C for 3 months. This inferred that sodium lactate can effectively be used as an alternative cryostabilizer to sucrose/sorbitol for stabilization of croaker muscle protein native structure.

Keywords: Protein denaturation, Dewatered mince meat, Physico-chemical properties, Frozen storage, Sodium lactate, Ca-ATPase activity

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References

  1. An H., Weerasinghe H.V., Seymour T.A., Morrissey M.T. Cathep sin degradation of Pacific whiting surimi proteins. J Food Sci. 1994;59:1013–1033. doi: 10.1111/j.1365-2621.1994.tb08179.x. [DOI] [Google Scholar]
  2. Official methods of analysis. 16th edn. Arlington, VA: Association of Official Analytical Chemists; 1995. [Google Scholar]
  3. Speck M. L., editor. Compendium of methods for the microbiological examination of foods. 2nd edn. Washington DC: American Public Health Association; 1984. [Google Scholar]
  4. Beatty S.A., Gibbons N.E. The measurement in spoilage of fish. J Biol Board Canada. 1937;3:77–91. [Google Scholar]
  5. Benjakul S., Bauer F. Physicochemical and enzymatic changes of cod muscle proteins subjected to different freeze thaw cycles. J Sci Food Agric. 2000;80:1143–1150. doi: 10.1002/1097-0010(200006)80:8<1143::AID-JSFA610>3.0.CO;2-C. [DOI] [Google Scholar]
  6. Benjakul S., Seymour T.S., Morrissey M.T., An H. Physicochemical changes in Pacific whiting muscle proteins during iced storage. J Food Sci. 1997;62:729–733. doi: 10.1111/j.1365-2621.1997.tb15445.x. [DOI] [Google Scholar]
  7. Benjakul S., Visessanguan W., Thongkaew C., Tanaka M. Comparative study on physico-chemical changes of muscle proteins from some tropical fish during frozen storage. Food Res Int. 2003;36:787–795. doi: 10.1016/S0963-9969(03)00073-5. [DOI] [Google Scholar]
  8. Borderias A.J., Jiménez-Colmenero J., Tejada M. Parameters affecting viscosity as a quality control for frozen fish. Marine Fish Rev. 1985;47:43–45. [Google Scholar]
  9. Chung K.H., Lee C.M. Water binding and ingredient dispersion pattern effects on surimi gel texture. J Food Sci. 1991;56:1263–1266. doi: 10.1111/j.1365-2621.1991.tb04748.x. [DOI] [Google Scholar]
  10. Dyer W.J., French H.V., Snow J.M. Proteins in fish muscle: Extraction of protein fraction in flesh. J Fish Res Board Canada. 1950;7:585–593. [Google Scholar]
  11. Fiske C.H., Subbarow Y. The colorimetric determination of phosphorus. J Biol Chem. 1925;66:375–400. [Google Scholar]
  12. Gopakumar K., Muraleedharan V., Bhattacharya S.K. Preparation and properties of surimi from tropical fish. Food Control. 1992;3:109–112. doi: 10.1016/0956-7135(92)90042-9. [DOI] [Google Scholar]
  13. Jacobs M.B. The chemical analysis of foods and food products. New York: Kreiger Publ Co. Inc.; 1958. pp. 393–394. [Google Scholar]
  14. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;337:680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Lowry O.H., Rosebrough N.J., Farr A.L., Randal R.J. Protein measurement with Folin phenol reagent. J Biol Chem. 1951;193:256–275. [PubMed] [Google Scholar]
  16. MacDonald G.A., Lanier T.C. Actomyosin stabilization to freeze-thaw and heat denaturation by lactate salts. J Food Sci. 1994;59:101–105. doi: 10.1111/j.1365-2621.1994.tb06907.x. [DOI] [Google Scholar]
  17. Matsumoto J. Chemical deterioration of muscle protein during frozen storage. In: Whitakers J.R., Fujinaki L.M., editors. Chemical deterioration of proteins. Washington DC: American Chemical Society; 1980. pp. 95–124. [Google Scholar]
  18. Mishra R., Srikar L.N. Shelf life of frozen stored clam meat. J Food Sci Technol. 1989;26:201–204. [Google Scholar]
  19. Niwa E. Chemistry of surimi gelation. In: Lanier T.C., Lee C.M., editors. Surimi technology. New York: Marcel Dekker Inc.; 1992. pp. 289–328. [Google Scholar]
  20. Ochaiai Y., Chow C. Myosin ATPase. In: Haard N.F., Simpson B.K., editors. Seafood enzymes utilization and influence on postharvest seafood quality. New York: Marcel Dekker Inc.; 2000. pp. 69–89. [Google Scholar]
  21. Park J.W., Lanier T.C. Combined effects of phos-phates and sugar or polyol on protein stabilization of fish myofibrils. J Food Sci. 1987;52:1509–1513. doi: 10.1111/j.1365-2621.1987.tb05866.x. [DOI] [Google Scholar]
  22. Park J.W., Morrissey M.T. Manufacturing of surimi from light muscle fish. In: Park J.W., editor. Surimi and surimi technology. New York: Marcel Dekker Inc.; 2000. pp. 28–43. [Google Scholar]
  23. Pigott G.M. Surimi: The’ High Tech’ raw materials from minced fish flesh. Food Rev Int. 1986;2:213–246. doi: 10.1080/87559128609540796. [DOI] [Google Scholar]
  24. Porteous J.D., Wood D.F. Water-binding of red meats in sausage formulation. Am Chem Soc Series. 1983;123:95–124. [Google Scholar]
  25. Reddy L., Setty T.M.R., Dora K.C. Studies on the storage behaviour of frozen fish fingers from croaker and perches. Fish Technol. 1992;29:35–39. [Google Scholar]
  26. Revankar G.D., Keshava N., Naidu A.K., Baliga B.L. Fish mince preparation and composition. Indian Food Packer. 1981;35(4):20–24. [Google Scholar]
  27. Sikorski Z.E., Olley J., Kostuch S. Protein changes in frozen fish. Crit Rev Food Sci Nutr. 1976;8:97–129. doi: 10.1080/10408397609527218. [DOI] [PubMed] [Google Scholar]
  28. Srikar L.N., Chandru R. Determination of organic nitrogen by Kjeldahl digestion using hydrogen peroxide. J Food Technol. 1983;18:129–133. doi: 10.1111/j.1365-2621.1983.tb00251.x. [DOI] [Google Scholar]
  29. Takagi T., Hayashi K., Itabashi Y. Toxic effect of free unsaturated fatty acid in mouse assay of diarrlutic shell fish toxin by intraperitoneal injection. Bull Jap Soc Sci Fish. 1984;50:1413–1418. [Google Scholar]

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