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. 2010 Jul 29;47(3):258–265. doi: 10.1007/s13197-010-0040-2

Simplification and optimization of deMan Rogosa Sharpe (MRS) medium for enhanced production of bacteriocin by Weissella paramesenteroides DFR-8

Ajay Pal 1,✉, K V Ramana 1, A S Bawa 1
PMCID: PMC3551021  PMID: 23572634

Abstract

Complex growth medium such as deMan Rogosa Sharpe (MRS) medium, commonly used for cultivation of fastidious lactic acid bacteria (LAB) interfere in bacteriocin purification. Sometimes all the ingredients of a defined medium are not required by all LAB strains for bacteriocin production. In the present study, composition of the MRS medium for the production of bacteriocin by Weissella paramesenteroides DFR-8, an isolate from cucumber (Cucumis sativus), was simplified and optimized with a step-wise strategy. In the first step, production profile, effect of incubation temperature, various C and N sources were investigated. In the second step, central composite rotatable design was employed to decide the optimal concentration of 3 key components (glucose, tryptone and pH) and the experimental results were fitted with a second order polynomial regression equation. According to the set criteria, the predicted bacteriocin titer from a medium containing 7.99% glucose, 9% tryptone, pH 7.5 (91.9% desirability) was 540 AU/ml and the observed bacteriocin titer was 538 AU/ml that indicated the validity of the developed model. Using optimized medium, bacteriocin titer of 674.5 AU/ml could be achieved after 72 h of fermentation that is nearly 2.5 fold higher than that obtained from unmodified MRS medium.

Keywords: Bacteriocin, Biopreservation, Optimization, Weissella paramesenteroides

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