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. 1973 Mar;11(3):368–371. doi: 10.1128/jvi.11.3.368-371.1973

Identification of the Block in the Intracellular Replication of Single-Stranded DNA of Photodynamically Inactivated Bacteriophage φX174

Utpal C Chaudhuri 1,1, Ramendra K Poddar 1
PMCID: PMC355111  PMID: 4570924

Abstract

32P-labeled single-stranded DNA phage φX174 was photodynamically inactivated by irradiation in air with visible light in the presence of the acridine dye, proflavine sulfate. The inactivated phages could adsorb to the host cells but failed to lyse them. Formation of intracellular mature phages was almost completely inhibited. Photodynamic lesions in φX174 DNA caused intracellular formation of defective double-stranded replicative form molecules which ultimately reverted to the single-stranded configuration.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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