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Journal of Food Science and Technology logoLink to Journal of Food Science and Technology
. 2011 Jan 18;48(5):525–533. doi: 10.1007/s13197-011-0229-z

Processing, food applications and safety of aloe vera products: a review

Kulveer Singh Ahlawat 1, Bhupender Singh Khatkar 1,✉
PMCID: PMC3551117  PMID: 23572784

Abstract

Aloe vera is used for vigor, wellness and medicinal purposes since rigvedic times. Health benefits of aloe vera include its application in wound healing, treating burns, minimizing frost bite damage, protection against skin damage from x-rays, lung cancer, intestinal problems, increasing high density lipoprotein (HDL), reducing low density lipoprotein (LDL), reducing blood sugar in diabetics, fighting acquired immune deficiency syndrome (AIDS), allergies and improving immune system. Phytochemistry of aloe vera gel has revealed the presence of more than 200 bioactive chemicals. Aloe vera gel is extracted from its leaves and appropriate processing techniques are needed for stabilization as well as preparation of the end products. The industries involved in processing of aloe vera need Government surveillance to ensure that the aloe vera products have beneficial bio-active chemicals as per claims of the manufacturers. Regulatory bodies also need to look into the safety and toxicological aspects of aloe vera products for food applications. The claims made for medicinal value of aloe products should be supported by authentic and approved clinical trial data. It is presumptive to mention that nutraceutical claims of aloe products made by the manufacturers are numerous. However, approved clinical evidences are available only for lowering LDL, increasing HDL, decreasing blood glucose level, treating genital herpes and psoriases.

Keywords: Aloe vera, Food applications, Medicinal value, Processing, Safety

Introduction

Aloe vera has been used for its medicinal value for several thousand years. Its applications have been recorded in ancient cultures of India, Egypt, Greece, Rome and China. In biblical times the Egyptians hailed aloe vera as the plant of immortality. The Chinese called it their elixir of youth. The aloe vera has many common names and often referred to as burn plant, first aid plant or medicine plant. Its name is most likely derived from the Arabic word “Alloeh” meaning shining bitter substance. Aloe vera is known by a number of names in the literature i.e. Aloe barbadensis Mill, Aloe chinensis Bak, Aloe elongate Murray, Aloe indica Royale, Aloe officinalis Forsk, Aloe perfoliata, Aloe rubescens DC, Aloe vera L. var. littoralis Konig ex Bak, Aloe vera L. var. chinensis Berger, Aloe vulgaris Lam. Most reference books regard Aloe barbadensis Mill as correct species name, and Aloe vera (L.) Burm f. as a synonym. However, according to the International Rules of Botanical Nomenclature, Aloe vera (L.) Burm f. is the legitimate name for this species. (Bradley 1992; Newton 1979; Tucker et al. 1989). The genus Aloe has also been placed taxonomically in a family called Aloeaceae (Farnsworth et al. 1999).

Aloe was originated in tropical Africa and it is now cultivated in warm climatic areas of Asia, Europe and America (Harding 1979). Presently, the use of aloe vera has gained popularity because of herbal movement initiated by naturopaths, yog gurus, alternative medicine promoters and holistic healers. The industry size for aloe raw material is estimated to be about $125 million dollars. The volume of the industry for finished products containing aloe vera is alleged to be around $110 billion dollars (Anonymous 2006). A recent market analysis report indicates that in 2008 Americans have spent almost 40 billion dollars on functional foods, drinks and supplements for the improvement of their appearance as well as to provide energy and added nutrition to handle health issues such as hypercholesterolemia and diabetes. Aloe vera products are among the popular ones for these applications. Today, the aloe vera industry is flourishing and the gel is used in many products such as fresh gel, juice and other formulations for health, medicinal and cosmetic purpose (Eshun and He 2004). However, the fast expanding aloe vera industry urgently needs reliable testing protocols to assess the quality and quantity of bioactive chemicals present in the final products (Bozzi et al. 2007). The product claims must be tested by intensive clinical trials, verified and certified by the Government regulatory authorities to built consumer confidence and safety of the aloe vera products.

Botany of aloe vera

Aloe vera is a spiky cactus like xerophytes. It is a clump forming perennial plant with thick fibrous root which produces large basal leaves, usually 12–16 per plant, weighing up to 1.5 kg when mature. The plant matures when it is about 4 years old and has a life span of about 12 years. The leaves are up to 0.5 m long and 8–10 cm across at the base, tapering to a point, with saw-like teeth along their margins. In a transverse section, the plant shows a slightly concave appearance on the adaxial surface and distinctly convex appearance on the lower abaxial surface (Grindlay and Reynolds 1986). The leaves are covered with thick cuticle, beneath which epidermis and mesophyll are present. Later is differentiated in upper chlorenchyma and lower parenchyma, as the rosette mature, successive leaves have fewer whitish spots and grey-greenish in colour (Eshun and He 2004).

The plant can be harvested every 6–8 weeks by removing 3–4 leaves per plant. Red, yellow, purple or pale stripped flowers are present most of the year growing in a long raceme at the top of the flower stalk which originates from the centre of the basal leaves. The flower stalk grows up to 1.5 m in height. The fruit is a triangular capsule containing numerous seeds. The plant is practically disease free, occasionally black spots may occur on upper surface because of fungal infection or soft rottening may damage whole plant. The causal organism for soft rottening is a bacterium. Frost is another enemy of aloe vera plant and it can not survive in frost conditions (Grindlay and Reynolds 1986). Smoking in field during frost nights is one measure practiced by farmers to protect the plantation from frost.

There are over 250 species of aloe grown world over. However, only two species are grown commercially i.e. Aloe barbadensis Miller (Aloe vera) and Aloe aborescens. There are at least two other species that have medicinal properties namely Aloe perry baker and Aloe ferox. Most aloe vera plants are non toxic but a few are extremely poisonous containing a hemlock like substance (Atherton 1998). Aloe variegate is a dwarf species which is only a few centimeter in diameter and is a popular house plant.

Phytochemistry of aloe vera

There are as many as 200 different types of molecules in aloe vera (Davis 1997). The aloe vera leaf gel contains about 98% water (Bozzi et al. 2007). The total solid content of aloe vera gel is 0.66% and soluble solids are 0.56% with some seasonal fluctuation. On dry matter basis aloe gel consists of polysaccharides (55%), sugars (17%), minerals (16%), proteins (7%), lipids (4%) and phenolic compounds (1%) (Fig. 1). The aloe vera gel contains many vitamins including the important antioxidant vitamins A, C and E. Vitamin B1 (thiamine), niacin, Vitamin B2 (riboflavin), choline and folic acid are also present (Lawless and Allen 2000). Some authors also suggested the presence of vitamins B12 (cyanocobalamin) in trace amounts which is normally available in animal source (Coats 1979; Atherton 1998).

Fig. 1.

Fig. 1

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Chemical composition of aloe vera gel (on dry weight basis) (Luta and McAnalley 2005)

Carbohydrates are derived from mucilage layer of the plant under the rind, surrounding the inner parenchyma or gel. They comprise both mono and polysaccharides. The most important are the long chain polysaccharides, comprising glucose and mannose, known as the glucomannans [β (1, 4) - linked acetylated mannan]. Xylose, rhamnose, galactose and arabinose are also present in trace amounts along with lupeol (a triterpenoid), cholesterol, campesterol and β-sitosterol. Structural studies on aloe vera gel polysaccharides have shown that the gel is composed of at least four different partially acetylated glucomannans, being linear polymers with no branching and having 1,4 glycosidic linkages with glucose and mannose in the ratio of 1:2:8. The viscosity of gel reduces upon hydrolysis of these sugars. When taken orally some of the sugars bind to receptor sites that line the gut and form a barrier, possibly helping to prevent ‘leaky gut syndrome’(Atherton 1997).

Other reports suggest the presence of glucose and a polyuronide consisting of a high molecular weight glucose mannose polyose (Mr 2.75 × 105) and hexouronic acid (Gjestad 1971). Ovadova et al. (1975) reported the presence of uronic acid, which gives galacturonic acid and oligosaccharides upon fermentative hydrolysis. Meadows (1980) reported that at least six enzymes are present in the aloe vera gel including bradykinase, cellulase, carboxypeptidase, catalase, amylase and oxidase. The carboxypeptidase inactivates bradykinase at site of wound or cut in body and produces pain relieving and anti-inflammatory effect. During the inflammatory process, bradykinase produces pain associated with vasodilatation (Shelton 1991). The gel also contains glutothionperoxidase as well as several isozymes of superoxide dismutase. Wang (1993) reported that potassium and chloride concentration appeared to be excessive in aloe vera juice in comparison to most plant products whereas the sodium content was found lesser in quantity. Calcium, magnesium, copper, zinc, chromium and iron were also found in the aloe products. Magnesium lactate inhibits histidine decarboxylase and prevents the formation of histamine from the amino acid histidine (Shelton 1991). Histamine is released in many allergic reactions and causes intense itching and pain. The prevention of its formation may explain the anti-allergic effects of aloe vera gel.

Anthraquinones are the phenolic compounds present in the sap or yellow exudates of leaf or aloe vera latex. Aloe latex contains a series of glycosides known as anthraquinones, the most prominent being aloin A and aloin B (Tyler 1994). The bitter aloes (dried yellow exudates) consists of free anthraquinones and their derivatives i.e. barbloin-IO-(1151-anhydroglucosyl)-aloe-emodin-9-anthrone, isobarbloin, anthrone–C- glycosides and chromones. These compounds exerts a powerful purgative effects when ingested in large amounts but when low in concentration, they appear to aid absorption from the gut and are potent antimicrobial (Sims et al. 1971) and powerful analgesic agents. Isolation and structure determinations of these chromones from the aloe vera leaves were also studied and these compounds were identified to be 8-C-glycosyl-7-O methyl-(S) aloesol, isoaloeresin D and aloeresin E (Saccu et al. 2001).

Recently, a glycoprotein with anti-allergic properties, called alprogen was isolated from aloe gel. In addition, a novel anti-inflammatory compounds, C-glycosyl chromones, has also been isolated from aloe gel (Hutter and Salman 1996). Saponins are the soapy substances, form 3% of the gel and are general cleansers, having antiseptic properties (Hirat and Suga 1983). The sterols include comperterol, β- sitosterol and lupeol (Coats 1979). Salicylic acid is an aspirin like compound possessing pain relieving properties (Table 1). About 20 out of 22 amino acids and seven of the eight essential amino acids required by human body are also present in aloe vera gel. Major phytochemicals occurring in aloe vera pulp and exudate are summarized in Table 2. Aloe vera juice was evaluated for antioxidant potential and the study showed significant presence of antioxidant in aloe extracts. A 3 years old plant extract exhibits the strongest free radical scavenging activity of 72.19%, which is significantly higher than that of BHT having 70.52% and α-tocopherol with 65.65% (Hu et al. 2003). It is suggested that growth stage in aloe plant plays a vital role in the composition and antioxidant activity (Hu et al. 2003). Aloe vera juice also has antibacterial properties against Gram- positive bacteria (Alemdar and Agaoglu 2009). Anonymous (2008) reported antiviral and antifungal properties of aloe vera.

Table 1.

Novel components of aloe vera along with their health benefits

Chemical component Health benefits
Acemannan Accelerate wound healing, modulate immune system, Antineoplastic and antiviral effects
Alprogen Anti-allergic
C-glycosyl chromone Anti-inflammatory
Bradykinase Anti-inflammatory
Magnesium lactate Anti-allergic
Salicylic acid Analgesic, anti-inflammatory
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Shelton (1991), Peng et al. (1991)

Table 2.

Summary of the phytochemicals of Aloe vera pulp and exudate

Class Compounds
Anthraquinones/anthrones Aloe-emodin, aloetic-acid, anthranol, aloin A and B (or collectively known as barbaloin), isobarbaloin, emodin, ester of cinnamic acid
Carbohydrates Pure mannan, acetylated mannan, acetylated glucomannan, glucogalactomannan, galactan, galactogalacturan, arabinogalactan, galactoglucoarabinomannan, pectic substance, xylan, cellulose
Chromones 8-C-glucosyl-(2′-O-cinnamoyl)-7-O-methylaloediol A, 8-C-glucosyl-(S)-aloesol, 8-C-glucosyl-7-O-methyl-(S)-aloesol, 8-C-glucosyl-7-O-methylaloediol, 8-C-glucosyl-noreugenin, isoaloeresin D, isorabaichromone, neoaloesin A
Enzymes Alkaline phosphatase, amylase, carboxypeptidase, catalase, cyclooxidase, cyclooxygenase, lipase, oxidase, phosphoenol, pyruvate carboxylase, superoxide dismutase
Minerals Calcium, chlorine, chromium, copper, iron, magnesium, manganese, potassium, phosphorous, sodium, zinc
Lipids and miscellaneous organic compounds Arachidonic acid, γ-linolenic acid, steroids (campestrol, cholesterol, β-sitosterol), triglicerides, triterpenoid, gibberillin, lignins, potassium sorbate, salicylic acid, uric acid
Amino acids Alanine, arginine, aspartic acid, glutamic acid, glycine, histidine, hydroxyproline, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tyrosine, valine
Proteins Lectins, lectin-like substance
Saccharides Mannose, glucose, L-rhamnose, aldopentose
Vitamins B1, B2, B6, C, β-carotene, choline, folic acid, α-tocopherol
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Ni and Tizard (2004), Dagne et al. (2000), Femenia et al. (1999), Choi and Chung (2003)

Processing of aloe vera

Aloe vera gel derived from the leaf pulp of the plant has become a big industry worldwide due to its application in the food industry. It is utilized in functional foods especially for the preparation of health drinks with no laxative effects. It is also used in other food products including milk, ice cream, confectionery, etc. Aloe vera gel is also used as flavoring component and preservative in some foods (Christaki and Florou-Paneri 2010). Thus, a simple and efficient processing technique needs to be developed especially for the aloe beverage industry to improve product quality and safety by preserving the bioactive chemicals naturally present in the intact aloe vera leaf (Eshun and He 2004).

The production process of aloe vera juice involves crushing, grinding or pressing of the entire leaf of the aloe vera plant to produce a liquid, followed by various steps of filtrations and stabilization (preserving the biological integrity of active ingredient to exert the reported physiological effect upon ingestion or topical application). The resulting juice is then incorporated in or mixed with other preparations or agents to produce a pharmaceutical, cosmetic or food product. In food industry, aloe vera has been utilized as a source of functional food drinks and other beverages including tea. The amount of aloe vera that finds its application in pharmaceutical industry is also substantial as evident by availability of topical ointments, gel preparations, tablets and capsules (Eshun and He 2004).

Unfortunately, because of improper processing procedures many of these so called aloe products contain very little or virtually no active ingredients namely, mucopolysaccharides. In view of known wide spectrum of biological activities possessed by the leaves of aloe vera plant and its wide spread use, it has become imperative that the leaf must be processed with the aim of retaining essential bioactive components up to maximum possible limit or as much as contained in fresh leaf. The general steps involved in the processing of aloe vera are explained in the following paragraphs.

Reception of raw material

The aloe vera leaves after harvesting must be transported in refrigerated vans from field to the processing plant. The leaves should be sound, undamaged, mold free and mature (3–4 years) in order to keep all the active ingredients in full concentration (Lawless and Allen 2000). One important factor affecting the composition of final product is the handling of the leaves after its harvesting because the decomposition of the gel matrix starts just after its cutting due to natural enzymatic reactions and the activity of bacteria normally present on the leaves. It can adversely affect the quality of the end product. Thus, the freshly removed leaves are refrigerated within 6 h or the leaves are directly fed to processing plant on the farm itself.

Filleting

In this process green rind of leaf is removed to extract the parenchymatous tissue called the gel fillet (Grindlay and Reynolds 1986). It is reported that the aloe gel extracted from the leaf had greater stability than the gel left in the leaf. In order to avoid the loss of biological activity filleting operation must be completed within 36 h of harvesting the leaves (Robert 1997). The presence of anthraquinones is an important factor leading to non enzymatic browning in aloe products (He et al. 2002).

Homogenization and enzymatic treatment

It includes crushing or grinding of gel fillet at room temperature (25 °C) in commercial high speed grinder. The crushing or grinding should be completed within 10–20 min in order to avoid the enzymatic browning. Enzymatic treatment of aloe vera gel for a long duration prior to processing is detrimental to polysaccharides (Gowda et al. 1980; Waller et al. 1978; Yagi et al. 1982). It has been reported that the enzyme treatment at 50 °C and within 20 min did not cause loss of biological activity of polysaccharide in aloe vera gel (Maughan 1984).

Filtration and deareation

Fibrous material is removed by this step. This operation influences the stability of aloe vera juice. Poor filtration results in sedimentation of aloe juice on storage. The unpasteurized aloe juice is fortified with vitamin C and citric acid to avoid browning reactions, improve flavor and stabilize the juice (Eison-Perchonok and Downes 1982; Kacem et al. 1987; Kennedy et al. 1992; Tramell et al. 1986). Aim of deareation is to prevent oxidation of ascorbic acid which eventually improves the flavors of aloe vera juice (Chan and Cavaletto 1986).

Hot processing and flash cooling

In hot processing, sterilization is achieved by treating the aloe liquid with the activated carbon at high temperature (Cerqveira et al. 1999). This step may affect the taste, appearance and the biological activity of aloe gel products. Biological activity of aloe vera gel essentially remains intact when gel is heated at 65 °C for a period less than 15 min. Extended periods or higher temperatures greatly reduce activity levels. After heat treatment, the juice is flash cooled to 5 °C or below within 15 s to preserve biological activity.

High temperature short time treatment (at 85–95 °C for 1–2 min) is an effective method to avoid the off flavor and the loss of biological activity of aloe vera gel. Physico-chemical modification promoted by heat treatment at different temperature range from 30 to 80 °C on acemannan was evaluated by Antoni et al. (2003). Heating promotes significant changes in the molecular weight of the bioactive polysaccharide increasing from 45 KDa in fresh aloe to 75 KDa for samples dehydrated at 70 and 80 °C. The physico-chemical alterations of the main type of polysaccharide may have important implications on the physiological activities attributed to the aloe vera plant.

Cold processing

In the cold processing technique, the entire processing steps are accomplished without the application of the heat. Coats (1994) reported the use of enzymes, like glucose oxidase and catalase to inhibit the growth of aerobic organisms within aloe vera gel and thereby sterilizing it. Other sterilization steps reported in the cold processing include exposing the gel to ultraviolet light followed by micron filtration (Maret 1975).

Addition of preservatives and stabilizers

In all the processing techniques, preservation can be achieved by the addition of chemical preservatives and other additives. The use of sodium benzoate, potassium sorbate, citric acid and Vitamin E in synergism has been reported by some researchers (Cerqveira et al. 1999; Moor and McAnalley 1995).

Stabilizing agent is added in aloe products to prevent sedimentation of juice upon storage. In an investigation (Yaron et al. 1992) the aloe vera gel was mixed with sulphited polysaccharides isolated from the red micro-algae, guar gum and xantham gum. Rheological studies indicated interaction of aloe vera gel with algal polysaccharides and xantham gum which is depicted by increased apparent viscosities, yield points and in some cases hysteresis but these interactions were not observed with guar gum. These desirable properties did not deteriorate during storage. It was, therefore, proposed that algal polysaccharides or xantham gum could stabilize the network structure of fresh aloe vera polysaccharide.

Storage

Aloe vera juice is packed in amber colored glass bottles to avoid the effect of light on the sensitive bioactive agents. Relative humidity and temperature are two most important environmental parameters that affect product quality. These two parameters can also affect the amount of the volatile substance of the juice absorbed by the packaging material (Hernadez and Giacin 1998) and consequently affect the shelf life of the product (Sadler and Braddock 1990).

Aloe juice and its food applications

Traditional method of aloe juice processing

In this method lower one inch of the leaf base, the tapering point (2–4 in.) of the leaf top, the short sharp spines located along the leaf margin as well as the top and bottom rind are removed with sharp knife along with the rind parts to which some mucilage remains attached. The fillet and the mucilage are collected from the aloe leaf for further processing. The highest concentrations of the potentially beneficial aloe constituents are found in mucilage as this layer represent the place of synthesis of the beneficial constituents. The material of the mucilage layer, subsequent to their synthesis, is distributed to the storage cells (cellulose-reinforced hexagons) of the fillet (Ramachandra and Srinivasa Rao 2008). The aloe vera gel fillet is washed with deionized water and transferred to the pulper. The pulper is fitted with refrigerated system that keeps the temperature of the extracted juice lower to prevent decomposition. The aloe vera juice is conveyed to a holding tank and kept for 24 h to decant. Holding tank is also refrigerated for preserving the bioactivity of sensitive molecules of aloe vera.

Whole leaf processing method

The process was developed in 1980’s in USA and undergone continuous improvement by contribution of different workers (Homcare Iberica 1983; Maughan 1984; Coats 1994). The procedure employs cold treatment to ensure product rich in bioactive compounds. In this process the base and tip of leaf are removed. The leaf is cut into sections and ground into particulate slurry in a Fiz Mill (Model D6 Make Arnold equipment company, Ohio) to produce a soup like consistency. The material is then treated with cellulase enzyme which breaks down the hexagonal structure of the fillet and releasing the cell constituents. The rind particles are removed by means of a series of coarse screening filters or passage through a juice press. This liquid is then pumped into large stainless steel sanitized holding tank. Once the tank is filled, it is hooked up to a depulping extractor. This machine removes the large pieces of pulp and rind which are generated by initial grinding process. Now the aloe liquid is passed through a series of filters that removes the aloin and aloe emodin as well as any microscopic traces of leaves, sand or other particles. A press filter is used for this purpose. The press filter’s carbon coated plates absorb the aloin and aloe emodin. Aloe liquid is continually passed through the filter press until the aloin and aloe emodin are removed. The filtered product is then placed in a second holding tank and passed through a press filter containing five micron filter paper. The aloe liquid is now ready for stabilization. This process can produce aloe vera juice containing three times more bio-active constituents than traditional hand filleted process (Ramachandra and Srinivasa Rao 2008).

The aloe vera juice finds wide application in food products like production of ready to serve drink, health drink, soft drink, laxative drink, aloe vera lemon juice, sherbet, aloe sports drink with electrolyte, diet drink with soluble fiber, hangover drink with B vitamin, amino acids and acetaminophen, healthy vegetable juice mix, tropical fruit juice with aloe vera, aloe vera yoghurts, aloe vera mix for whiskey and white bread, cucumber juice with aloe vera (Eshun and He 2004; Hamman 2008; Grindlay and Reynolds 1986; Ang et al. 1996; Hastuti 1999; Singh and Singh 2009) (Table 3). Wei et al. (2004) prepared a health beverage from fresh aloe vera leaves. The leaves were washed, pulped, sterilized and filtered, then mixed with different concentrations of Dangshen, Maidong juices and Chinese herbs. Effects of processing conditions e.g. temperature, pH, sucrose, vitamin C and citric acid on the stability of colour and gelatinoids in aloe vera juice were studied and it was concluded that the stability was negatively affected by increasing sucrose and citric acid concentrations while vitamin C and sodium chloride at low concentrations improved the stability. Do-sang et al. (1999) prepared vinegar from aloe vera juice using Acetobactor sp. Lee and Hand-Yoon (1997) made aloe vera yoghurt with lactic acid bacteria (single or mixed strains of Lactobacillus bulgaricus and Streptococcus thermophilus) and compared it with yoghurt prepared using dried skim milk and it was found that quality retention of aloe vera yoghurt at 5 °C for 15 days was better than the milk yoghurt.

Table 3.

Food applications of aloe vera products

Aloe vera products Food applications
Concentrate Squash, jam, jellies, aloe vera concentrate can also be mixed with tea, water or juice
Gel fillet Candies, bar, munch, chewing gum, instant aloe vera tea granules, aloe vera gum for sore or bleeding gums, candy type aloe vitamins, aloe vera fruit smoothies
Juice Ready to serve drink, health drink, soft drink, laxative drink, sherbet, sports drink (with electrolytes), diet drink with soluble fiber, hangover drink with B- vitamins, amino acids and acetaminophen, healthy vegetable juice mix, yoghurts, aloe vera mix for whiskey or other alcohol, white bread with aloe vera, and cucumber juice with aloe vera
Powder Yoghurt, curd, ‘lassi’, ice-cream, and aloe vera ‘laddu’
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Eshun and He (2004), Hamman (2008), Grindlay and Reynolds (1986), Ang et al. (1996), Hastuti (1999), Singh and Singh (2009)

Aloe concentrate and its food applications

The aloe juice can be concentrated under vacuum without the loss of biological activity. The concentration operation must be conducted under 125 mm Hg vacuum at temperature below 50 °C and must not exceed 2 min as higher vacuum and temperature will cause loss of effectiveness of bioactive constituents (Ramachandra and Srinivasa Rao 2008). Concentration is carried out to get aloe vera concentrate of desired consistency to suit various food applications i.e. squash, jam and jellies. The concentrate of aloe can also be mixed with tea, water or juice.

Aloe powder and its food applications

In dehydration method the pure intact aloe vera gel fillets are first washed to remove traces of aloin. Then the fillets are placed into a humidity chamber where desired level of relative humidity and temperature are maintained (Ramachandra and Srinivasa Rao 2008). Here hot air is passed over the fillets to dry them. This material is then ground to powder and packed (Ramachandra and Srinivasa Rao 2008).

Qmatrix drying is a novel proprietary method of dehydration of aloe vera enabling the dehydration of aloe while maintaining its integrity with respect to flavor, colour and bioactivity. It is comparable to freeze drying in quality aspects but without the high operational cost. In freeze drying, gel fillet is lyophilized at −88 °C and 0.01 mm Hg pressure for 65 h to get dried gel fillet. Later is then ground to get aloe powder with moisture content below 4%. Qian (2002) prepared freeze dried powder from ultra-filtration and reverse osmosis of concentrated aloe vera gel. Gautam and Awasthi (2007) prepared aloe vera leaf powder by cutting leaves in small pieces, blending in a mixer and drying them in a tray drier at 50 °C for 12 h. The dried material is then ground into powder in a mixer grinder. Aloe vera powder can be used in curd, lassi, ice-creams, etc. Aloe powder has also been used in the preparation of yoghurts (Lee and Choi 1994; Seoshin et al. 1995)

Safety aspects of aloe vera products

Scientific community is divided into two groups regarding safety of aloe vera products. One group advocates that the aloe vera is quite safe for human consumption. While the other group warns to use it with caution and utmost care to avoid contamination of aloin from the yellow exudates, as aloin is reported as DNA damaging and cancer causing (Lachenmeier et al. 2005). On the contrary scientists have reported that anthroquinones present in aloe vera leaf, including aloin, are beneficial in a number of ways when used in small quantity, though the small quantity is not well defined (Sydiskis et al. 1991).

It is reported that aloe vera gel is safe for external use, allergies are rare and adverse reactions with other medications have not been reported. Aloe should not be used internally during pregnancy, lactation or childhood and by persons suffering from abdominal pain, appendicitis or intestinal obstruction (Kemper and Chiou 1999).

A case of disseminated dermatitis has been reported following application of aloe vera gel to a patient with stasis dermatitis. Several patients who applied aloe vera gel topically following dermabrasion reported burning sensation and development of dermatitis on the face (Hunter and Frumkin 1991). Because of possible contamination by anthraquinones, oral aloe gel may cause symptoms of abdominal cramps and diarrhoea. There have also been several reports of aloe vera gel lowering plasma glucose in laboratory animal and in human (Ghannam et al. 1986). Use of aloe vera is reportedly associated with occurrence of Henoch-Schonlein purpura (HSP) a systemic vasculitis that occurs most often in children who are rarely exposed to drugs or other environmental factors. Acute hepatitis could be linked to ingestion of Aloe barbdensis compounds. An acute bullous allergic reaction and urticaria have also been reported to result from the use of aloe vera gel (Morrow et al. 1980).

Studies in mice revealed no acute toxicity in therapeutic doses but in high doses a decreased central nervous system (CNS) activity was noticed (Shah et al. 1989). In chronic treatment decrease in red cell count and significant sperm damage was noticed (Shah et al. 1989). However, no systematic investigation exists in humans on the effect of high doses of aloe vera for longer periods on red cell count and sperm damage (Vogler and Ernst 1999).

Conclusions

Reported therapeutic benefits of aloe vera products are numerous such as helping wound healing, treating burns, minimizing frost bite damage, protection against skin damage from X-rays, lung cancer, intestinal problems. However, approved clinical trial data support its effectiveness for lowering LDL, increasing HDL, decreasing blood glucose level, treating genital herpes and psoriases. Scientific evidences suggest that aloe vera gel is safe for external use, allergies are rare and adverse reactions with other medications have not been reported. However, it is recommended that aloe products should not be used internally during pregnancy or lactation period by women and by persons suffering from abdominal pain, appendicitis or intestinal obstruction. The aloe vera industry is flourishing worldwide and the gel is used in many products including fresh gel, juice and other formulations for health, medicinal and cosmetic purpose. However, the fast expanding aloe vera industry urgently needs reliable testing protocols to assess the quality and quantity of bioactive chemicals present in the final products. The product claims must be tested by intensive clinical trials, verified and certified by the Government regulatory authorities to build consumer confidence and to ensure safety of the aloe vera products.

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