Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1973 Apr;11(4):473–478. doi: 10.1128/jvi.11.4.473-478.1973

Inhibition of Replication of Lactic Dehydrogenase Virus by Actinomycin

Shudo Yamazaki 1,1, Abner L Notkins 1
PMCID: PMC355127  PMID: 4349489

Abstract

Lactic dehydrogenase virus replicated rapidly in monolayers of primary mouse embryo cells and reached a titer of 108 mean infective dose per ml within 18 h after infection. Despite the high virus yield, cytopathology was not observed. Examination of the tissue culture media failed to reveal any evidence of interferon, but the virus was found to be as sensitive to mouse interferon as vesicular stomatitis virus. Incubation of mouse embryo cells with actinomycin D markedly inhibited viral replication, whereas cytosine-β-d-arabinofuranoside and 5-fluorodeoxyuridine had no effect on replication. These findings indicate that new DNA synthesis is not required but suggest that the intact function of cellular DNA may be required for lactic dehydrogenase virus replication.

Full text

PDF
473

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson H. C., Riley V., Fitzmaurice M. A., Loveless J. D., Wade P., Moore A. E. Quantitative study of the lactate dehydrogenase-elevating virus in mouse embryo cultures. J Natl Cancer Inst. 1966 Jan;36(1):89–95. [PubMed] [Google Scholar]
  2. BADER J. P. THE REQUIREMENT FOR DNA SYNTHESIS IN THE GROWTH OF ROUS SARCOMA AND ROUS-ASSOCIATED VIRUSES. Virology. 1965 Jun;26:253–261. doi: 10.1016/0042-6822(65)90272-2. [DOI] [PubMed] [Google Scholar]
  3. BARON S., DUBUY H. G., BUCKLER C. E., JOHNSON M. L. RELATIONSHIP OF INTERFERON PRODUCTION TO VIRUS GROWTH IN VIVO. Proc Soc Exp Biol Med. 1964 Nov;117:338–341. doi: 10.3181/00379727-117-29575. [DOI] [PubMed] [Google Scholar]
  4. BARRY R. D. THE EFFECTS OF ACTINOMYCIN D AND ULTRAVIOLET IRRADIATION ON THE PRODUCTION OF FOWL PLAGUE VIRUS. Virology. 1964 Dec;24:563–569. doi: 10.1016/0042-6822(64)90208-9. [DOI] [PubMed] [Google Scholar]
  5. Borland R., Mahy B. W. Deoxyribonucleic acid-dependent ribonucleic acid polymerase activity in cells infected with influenza virus. J Virol. 1968 Jan;2(1):33–39. doi: 10.1128/jvi.2.1.33-39.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Buck L. L., Pfau C. J. Inhibition of lymphocytic choriomeningitis virus replication by actinomycin D and 6-azauridine. Virology. 1969 Apr;37(4):698–701. doi: 10.1016/0042-6822(69)90294-3. [DOI] [PubMed] [Google Scholar]
  7. Cohen S. S., Flaks J. G., Barner H. D., Loeb M. R., Lichtenstein J. THE MODE OF ACTION OF 5-FLUOROURACIL AND ITS DERIVATIVES. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1004–1012. doi: 10.1073/pnas.44.10.1004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cooper P. D. The inhibition of poliovirus growth by actinomycin D and the prevention of the inhibition by pretreatment of the cells with serum or insulin. Virology. 1966 Apr;28(4):663–678. doi: 10.1016/0042-6822(66)90251-0. [DOI] [PubMed] [Google Scholar]
  9. Du Buy H. G., Johnson M. L. Studies on the in vivo and in vitro multiplication of the LDH virus of mice. J Exp Med. 1966 Jun 1;123(6):985–998. doi: 10.1084/jem.123.6.985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. DuBuy H. G., Johnson M. L. Effect of actinomycin D on lactic dehydrogenase virus multiplication in mouse macrophages. Proc Soc Exp Biol Med. 1970 Mar;133(3):1023–1025. doi: 10.3181/00379727-133-34618. [DOI] [PubMed] [Google Scholar]
  11. Evans R. Further studies on the replication of the lactate dehydrogenase-elevating virus (LDH virus) in mouse peritoneal macrophage cultures. Proc Soc Exp Biol Med. 1970 Mar;133(3):831–836. doi: 10.3181/00379727-133-34574. [DOI] [PubMed] [Google Scholar]
  12. Evans R. Replication of Riley's plasma enzymes elevating virus in tissue culture: the importance of the cellular composition. J Gen Virol. 1967 Jul;1(3):363–374. [PubMed] [Google Scholar]
  13. Evans R., Riley V. Circulating interferon in mice infected with the lactate dehydrogenase-elevating virus. J Gen Virol. 1968 Dec;3(3):449–452. doi: 10.1099/0022-1317-3-3-449. [DOI] [PubMed] [Google Scholar]
  14. GEORGII A., LENZ I. FAILURE TO PROPAGATE A LACTIC DEHYDROGENASE-ELEVATING AGENT FROM MICE TUMOURS IN MICE EMBRYO CULTURES. Nature. 1964 Jun 20;202:1228–1229. doi: 10.1038/2021228a0. [DOI] [PubMed] [Google Scholar]
  15. Grado C., Fischer S., Contreras G. The inhibition by actinomycin D of poliovirus multiplication HEp 2 cells. Virology. 1965 Dec;27(4):623–625. doi: 10.1016/0042-6822(65)90189-3. [DOI] [PubMed] [Google Scholar]
  16. HELLER E. ENHANCEMENT OF CHIKUNGUNYA VIRUS REPLICATION AND INHIBITION OF INTERFERON PRODUCTION BY ACTINOMYCIN D. Virology. 1963 Dec;21:652–656. doi: 10.1016/0042-6822(63)90239-3. [DOI] [PubMed] [Google Scholar]
  17. NOTKINS A. L. RECOVERY OF AN INFECTIOUS RIBONUCLEIC ACID FROM THE LACTIC DEHYDROGENASE AGENT BY TREATMENT WITH ETHER. Virology. 1964 Apr;22:563–567. doi: 10.1016/0042-6822(64)90077-7. [DOI] [PubMed] [Google Scholar]
  18. NOTKINS A. L., SHOCHAT S. J. Studies on the multiplication and the properties of the lactic dehydrogenase agent. J Exp Med. 1963 May 1;117:735–747. doi: 10.1084/jem.117.5.735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Notkins A. L. Enzymatic and immunologic alterations in mice infected with lactic dehydrogenase virus. Am J Pathol. 1971 Sep;64(3):733–746. [PMC free article] [PubMed] [Google Scholar]
  20. Notkins A. L., Mahar S., Scheele C., Goffman J. Infectious virus-antibody complex in the blood of chronically infected mice. J Exp Med. 1966 Jul 1;124(1):81–97. doi: 10.1084/jem.124.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Oldstone M. B., Dixon F. J. Lactic dehydrogenase virus-induced immune complex type of glomerulonephritis. J Immunol. 1971 May;106(5):1260–1263. [PubMed] [Google Scholar]
  22. Plagemann P. G., Swim H. E. Propagation of lactic dehydrogenase-elevating virus in cell culture. Proc Soc Exp Biol Med. 1966 Apr;121(4):1147–1152. doi: 10.3181/00379727-121-30991. [DOI] [PubMed] [Google Scholar]
  23. Porter D. D., Porter H. G. Deposition of immune complexes in the kidneys of mice infected with lactic dehydrogenase virus. J Immunol. 1971 May;106(5):1264–1266. [PubMed] [Google Scholar]
  24. REVEL M., HIATT H. H., REVEL J. P. ACTINOMYCIN D: AN EFFECT ON RAT LIVER HOMOGENATES UNRELATED TO ITS ACTION ON RNA SYNTHESIS. Science. 1964 Dec 4;146(3649):1311–1313. doi: 10.1126/science.146.3649.1311. [DOI] [PubMed] [Google Scholar]
  25. Reich E., Goldberg I. H. Actinomycin and nucleic acid function. Prog Nucleic Acid Res Mol Biol. 1964;3:183–234. doi: 10.1016/s0079-6603(08)60742-4. [DOI] [PubMed] [Google Scholar]
  26. Schaffer F. L., Gordon M. Differential inhibitory effects of actinomycin D among strains of poliovirus. J Bacteriol. 1966 Jun;91(6):2309–2316. doi: 10.1128/jb.91.6.2309-2316.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Stewart W. E., 2nd, Scott W. D., Sulkin S. E. Relative sensitivities of viruses to different species of interferon. J Virol. 1969 Aug;4(2):147–153. doi: 10.1128/jvi.4.2.147-153.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. WHITE D. O., DAY H. M., BATCHELDER E. J., CHEYNE I. M., WANSBROUGH A. J. DELAY IN THE MULTIPLICATION OF INFLUENZA VIRUS. Virology. 1965 Feb;25:289–302. doi: 10.1016/0042-6822(65)90207-2. [DOI] [PubMed] [Google Scholar]
  29. YAFFE D. The distribution and in vitro propagation of an agent causing high plasma lactic dehydrogenase activity. Cancer Res. 1962 Jun;22:573–580. [PubMed] [Google Scholar]
  30. Yamazaki S. Effect of endogenous interferon and actinomycin D on growth of Japanese encephalitis virus in chick embryo cells. Jpn J Microbiol. 1968 Jun;12(2):171–178. doi: 10.1111/j.1348-0421.1968.tb00381.x. [DOI] [PubMed] [Google Scholar]
  31. Yamazaki S., Wagner R. R. Purified rabbit interferon: attempts to demonstrate interferon-specific 3H-protein. J Virol. 1970 Feb;5(2):270–273. doi: 10.1128/jvi.5.2.270-273.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES