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. 1973 Apr;11(4):508–514. doi: 10.1128/jvi.11.4.508-514.1973

Analysis of Simian Virus 40 DNA with the Restriction Enzyme of Haemophilus aegyptius, Endonuclease Z

Eng-Shang Huang 1, John E Newbold 1, Joseph S Pagano 1
PMCID: PMC355131  PMID: 4349491

Abstract

Limited digestion of simian virus 40 (SV40) DNA from both small- and large- plaque strains with the restriction endonuclease Z from Haemophilus aegyptius yielded 10 specific fragments. The number of nucleotide pairs for each fragment, determined by co-electrophoresis with φX174 RF fragments produced by endonuclease Z, ranges from 2,050 to 80. The difference in the pattern between the large- and small-plaque strains is the disappearance of one fragment containing approximately 255 nucleotide pairs and the appearance of a new fragment with 145 nucleotide pairs. This finding can be explained either by deletions or insertions totaling 110 nucleotide pairs. Complementary RNA synthesized in vitro from the adeno-SV40 hybrid virus, strain ND-1, hybridized preferentially to four of the fragments of SV40 DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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