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. 1973 Apr;11(4):515–519. doi: 10.1128/jvi.11.4.515-519.1973

Surface Antigens on Cat Leukemic Cells Induced by Feline Leukemia Virus: Area Density and Antibody-Binding Affinity

Charles W Boone 1,2, Fred Gordin 1,2, Thomas G Kawakami 1,2
PMCID: PMC355132  PMID: 4349492

Abstract

The binding of autologous bovine antibody to feline leukemia virus-induced cell surface antigens (FeCSA) on cat leukemia cells was studied by performing certain titration procedures with a mixture of immune and normal sera labeled with different iodine radioisotopes (paired-label technique). By using plots of titration data which conformed to linear equations derived from the mass action law, we determined the following constants. (i) The density of FeCSA was 2.03 × 106 sites per cell, or 5,230 sites per μm2. (ii) The equilibrium constant of the FeCSA-antibody reaction was 2.67 × 107, from which the antibody binding affinity or standard free energy of the FeCSA-antibody bond was determined to be − 10.48 kcal (−43,869.28 J) per mol. The use of the techniques described to measure the concentration of antibody in antiserum, in micrograms per milliliter, is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aoki T., Boyse E. A., Old L. J., De Harven E., Hämmerling U., Wood H. A. G (Gross) and H-2 cell-surface antigens: location on Gross leukemia cells by electron microscopy with visually labeled antibody. Proc Natl Acad Sci U S A. 1970 Mar;65(3):569–576. doi: 10.1073/pnas.65.3.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boone C. W., Brandchaft P. R., Irving D. N., Gilden R. Quantitative studies on the binding of syngeneic antibody to the surface antigens of AKR-virus-induced rat lymphoma cells. Int J Cancer. 1972 May 15;9(3):685–692. doi: 10.1002/ijc.2910090327. [DOI] [PubMed] [Google Scholar]
  3. Boone C. W., Irving D. N., Rubinstein S. Quantitative studies on the binding of antibody to the surface of HeLa cells. J Immunol. 1971 Apr;106(4):879–887. [PubMed] [Google Scholar]
  4. Davis W. C. H-2 antigen on cell membranes: an explanation for the alteration of distribution by indirect labeling techniques. Science. 1972 Mar 3;175(4025):1006–1008. doi: 10.1126/science.175.4025.1006. [DOI] [PubMed] [Google Scholar]
  5. Economidou J., Hughes-Jones N. C., Gardner B. Quantitative measurements concerning A and B antigen sites. Vox Sang. 1967 May;12(5):321–328. doi: 10.1111/j.1423-0410.1967.tb03362.x. [DOI] [PubMed] [Google Scholar]
  6. HUGHES-JONES N. C., GARDNER B., TELFORD R. STUDIES ON THE REACTION BETWEEN THE BLOOD-GROUP ANTIBODY ANTI-D AND ERYTHROCYTES. Biochem J. 1963 Sep;88:435–440. doi: 10.1042/bj0880435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HUGHES-JONES N. C., GARDNER B., TELFORD R. The kinetics of the reaction between the blood-group antibody anti-c and erythrocytes. Biochem J. 1962 Dec;85:466–474. doi: 10.1042/bj0850466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hämmerling U., Eggers H. J. Quantitative measurement of uptake of alloantibody on mouse lymphocytes. Eur J Biochem. 1970 Nov;17(1):95–99. doi: 10.1111/j.1432-1033.1970.tb01140.x. [DOI] [PubMed] [Google Scholar]
  9. Oshiro L. S., Riggs J. L., Taylor D. O., Lennette E. H., Huebner R. J. Ferritin-labeled antibody studies of feline C-type particles. Cancer Res. 1971 Aug;31(8):1100–1110. [PubMed] [Google Scholar]
  10. Sarma P. S., Baskar J. F., Gilden R. V., Gardner M. B., Huebner R. J. In vitro isolation and characterization of the GA strain of feline sarcoma virus. Proc Soc Exp Biol Med. 1971 Sep;137(4):1333–1336. doi: 10.3181/00379727-137-35783. [DOI] [PubMed] [Google Scholar]
  11. Sarma P. S., Gilden R. V., Huebner R. J. Complement-fixation test for feline leukemia and sarcoma viruses (the COCAL test). Virology. 1971 Apr;44(1):137–145. doi: 10.1016/0042-6822(71)90160-7. [DOI] [PubMed] [Google Scholar]
  12. Sarma P. S., Log T., Theilen G. H. ST feline sarcoma virus. Biological characteristics and in vitro propagation. Proc Soc Exp Biol Med. 1971 Sep;137(4):1444–1448. doi: 10.3181/00379727-137-35807. [DOI] [PubMed] [Google Scholar]
  13. Theilen G. H., Kawakami T. G., Rush J. D., Munn R. J. Replication of cat leukemia virus in cell suspension cultures. Nature. 1969 May 10;222(5193):589–590. doi: 10.1038/222589b0. [DOI] [PubMed] [Google Scholar]

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