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. 1973 Apr;11(4):527–534. doi: 10.1128/jvi.11.4.527-534.1973

Effect of Inhibition of Macromolecule Synthesis on the Association of Bacteriophage T4 DNA with Membrane

C F Earhart A, C J Sauri A,1, Gail Fletcher A, Judith L Wulff A
PMCID: PMC355134  PMID: 4573364

Abstract

The “Mg2+-Sarkosyl crystals” (M band) technique distinguishes between membrane-bound and free intracellular DNA. This procedure was employed to investigate the nature of the reactions necessary to convert input T4 DNA to a rapidly sedimenting form. Energy poisoning inhibits this attachment reaction. Neither protein nor DNA synthesis appears to be required, but experiments with rifampin and extensively irradiated T4 suggest that RNA synthesis is involved. These results were confirmed by a second procedure for the determination of rapidly sedimenting DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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