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. 1973 Apr;11(4):535–543. doi: 10.1128/jvi.11.4.535-543.1973

Identification of Polysomal RNA in BHK Cells Infected by Sindbis Virus

Deborah Mowshowitz 1
PMCID: PMC355135  PMID: 4633684

Abstract

Polysomes were prepared from Sindbis virus-infected BHK cells. The major species of RNA in these polysomes was identified as 26S RNA (interjacent RNA) by (i) disrupting the polysomes with EDTA; (ii) treating the infected cells with puromycin; and (iii) isolating polysomes from cells infected with a temperature-sensitive mutant that does not form nucleocapsids. Small amounts of 42S RNA and 33S RNA were also found in polysomes.

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Selected References

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