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. 1973 May;11(5):615–620. doi: 10.1128/jvi.11.5.615-620.1973

Molecular Weight Determination of Sendai RNA by Dimethyl Sulfoxide Gradient Sedimentation

Daniel Kolakofsky 1, Andrée Bruschi 1
PMCID: PMC355155  PMID: 4350707

Abstract

The molecular weight of the large RNA of Sendai virus has been determined by sedimentation analysis in sucrose gradients containing 99% dimethyl sulfoxide (DMSO) to be 2.3 × 106. Sendai RNA recovered from 99% DMSO was found to cosediment with nondenatured Sendai RNA at 46 to 48s in ordinary sucrose gradients. The molecular weight value of 2.3 × 106 is considerably smaller than the estimates of 6 × 106 to 7 × 106 determined under nondenaturing conditions, suggesting a unique structure for Sendai RNA.

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Selected References

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