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. 1973 May;11(5):621–629. doi: 10.1128/jvi.11.5.621-629.1973

Growth of Coliphage T7 in Salmonella typhimurium

Ilze Brunovskis a,1, R O Burns a
PMCID: PMC355156  PMID: 4350708

Abstract

A mutant of Salmonella typhimurium was found to be sensitive to killing by coliphage T7 because of an alteration in its surface properties. However, the infections were abortive and studies with 32P-labeled T7 grown in Escherichia coli B (T7·B) indicated that the phage DNA was restricted by S. typhimurium. When a mutant T7 which survived the restriction and produced plaques on Salmonella (T7·S) was passed through one cycle of growth in E. coli B, its ability to grow in Salmonella was lost, indicating that host-controlled restriction and modification are operative in this system. Restrictionless S. typhimurium mutants were isolated that permit the growth of not only T7·S but also T7·B and coliphage T3. The physiology of T7 production in the restrictionless host is nearly identical to that in Escherichia coli.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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